cDNA Clone PIAS1

• Synonyms: protein inhibitor of activated STAT 1, GBP, Ddxbp1, 2900068C24Rik
• Plasmid: pcDNA5-FRT/TO-EYFP-PIAS1
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: EYFP-PIAS1
• Genbank: BAC35902 Gene ID: 56469
• Tag: YFP
• Species: Mouse

Sequence of Insert

ggatccATGGCGGACAGTGCGGAACTAAAGCAAATGGTTATGAGCCTTAG
AGTTTCTGAACTCCAAGTACTGTTGGGCTACGCTGGGAGGAACAAGCACG
GACGCAAACACGAACTTCTTACAAAAGCCCTGCATTTGTTAAAGGCTGGC
TGTAGTCCTGCTGTACAAATGAAAATTAAAGAACTCTACAGGAGGCGGTT
CCCTCAGAAAATTATGACGCCTGCGGACTTGTCTATCCCCAACGTACATT
CAAGTCCTATGCCTCCGACTCTTTCTCCATCCACCATTCCACAGCTCACT
TATGATGGCCACCCTGCATCATCCCCACTACTCCCTGTTTCTCTTCTGGG
ACCCAAACATGAACTGGAACTCCCACATCTCACGTCAGCGCTGCACCCAG
TCCACCCGGACATAAAGCTGCAGAAGCTACCATTCTATGACCTGTTGGAT
GAACTGATCAAGCCCACCAGTCTAGCTTCAGACAACAGCCAGCGCTTTCG
GGAAACCTGTTTTGCATTTGCCTTGACACCACAACAGGTGCAGCAGATCA
GCAGCTCCATGGATATTTCTGGGACCAAATGTGACTTCACAGTGCAGGTC
CAATTAAGGTTTTGTTTATCAGAAACCAGTTGTCCACAAGAAGATCACTT
CCCACCCAACCTTTGTGTAAAAGTGAATACAAAACCTTGCAGCCTTCCAG
GTTACCTTCCACCTACTAAAAACGGTGTGGAACCAAAGCGACCTAGCCGA
CCAATTAATATCACCTCACTTGTCCGATTGTCCACGACAGTACCAAATAC
CATTGTTGTTTCTTGGACTGCAGAAATTGGAAGAACCTATTCCATGGCAG
TATATCTTGTAAAACAGTTGTCCTCAACAGTTCTTCTTCAGAGGTTACGA
GCAAAGGGAATAAGGAATCCGGATCATTCTAGAGCTTTAATTAAAGAGAA
GTTAACTGCAGATCCAGATAGTGAGATAGCTACTACCAGCCTACGGGTTT
CGCTGCTGTGTCCACTTGGGAAAATGCGACTGACAATCCCCTGTCGGGCA
CTTACCTGCTCCCACCTTCAGTGTTTTGATGCAACTCTTTACATTCAAAT
GAATGAGAAAAAACCAACATGGGTTTGTCCTGTCTGTGATAAGAAGGCCC
CATATGAACACCTTATTATTGACGGGTTGTTTATGGAAATTCTAAAGTAC
TGCACAGACTGTGACGAGATACAGTTTAAGGAGGATGGCTCGTGGGCTCC
AATGAGGTCAAAGAAGGAGGTTCAAGAAGTCACTGCCTCCTACAATGGAG
TTGATGGTTGCTTGAGCTCCACATTGGAGCATCAGGTAGCGTCCCACAAC
CAGTCCTCAAATAAAAACAAGAAAGTCGAGGTCATTGACCTAACCATTGA
CAGCTCGTCAGATGAAGAGGAGGAAGAACCCCCTGCCAAGAGGACCTGTC
CTTCCCTGTCTCCTACGTCACCACTAAGTAATAAAGGCATTTTAAGTCTT
CCTCATCAAGCCTCGCCTGTGTCCCGCACCCCAAGCCTTCCTGCTGTAGA
TACAAGCTACATCAACACCTCCCTCATCCAGGACTACAGGCACCCCTTCC
ACATGACGCCTATGCCTTATGACTTACAAGGATTAGATTTCTTTCCTTTC
TTATCAGGAGACAATCAGCATTACAACACCTCCCTGCTAGCCGCAGCTGC
AGCGGCGGTCTCAGATGACCAGGACCTCCTGCACTCCTCCCGGTTTTTCC
CGTATACCTCCTCGCAGATGTTTCTCGACCAGCTAAGTGCAGGAGGGAGC
ACATCTCTGCCAGCCACCAACGGAAGCAGTAGCGGCAGCAACAGCAGCCT
TGTGTCTTCCAACAGTCTGAGAGAGAGCCATGGCCATGGTGTGGCCAGCA
GGAGCAGCGCAGACACAGCGTCCATCTTTGGCATCATACCAGACATTATC
TCATTGGACTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSMADSAELKQ
MVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKE
LYRRRFPQKIMTPADLSIPNVHSSPMPPTLSPSTIPQLTYDGHPASSPLL
PVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASD
NSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSC
PQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLS
TTVPNTIVVSWTAEIGRTYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSR
ALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDA
TLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKE
DGSWAPMRSKKEVQEVTASYNGVDGCLSSTLEHQVASHNQSSNKNKKVEV
IDLTIDSSSDEEEEEPPAKRTCPSLSPTSPLSNKGILSLPHQASPVSRTP
SLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTS
LLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPATNGSSS
GSNSSLVSSNSLRESHGHGVASRSSADTASIFGIIPDIISLD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24516
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Constructed by Anne Seifert (Ron Hay Grp)