cDNA Clone PIAS4

• Plasmid: pcDNA5-FRT/TO-EYFP-PIAS4
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: EYFP-PIAS4
• Genbank: BC029874 NM_015897.2
• Tag: YFP
• Species: Human

Sequence of Insert

ggatccAAGATGGCGGCGGAGCTGGTGGAGGCCAAAAACATGGTGATGAG
TTTTCGAGTCTCCGACCTTCAGATGCTCCTGGGTTTCGTGGGCCGGAGTA
AGAGTGGACTGAAGCACGAGCTCGTCACCAGGGCCCTCCAGCTGGTGCAG
TTTGACTGTAGCCCTGAGCTGTTCAAGAAGATCAAGGAGCTGTACGAGAC
CCGCTACGCCAAGAAGAACTCGGAGCCTGCCCCACAGCCGCACCGGCCCC
TGGACCCCCTGACCATGCACTCCACCTACGACCGGGCCGGCGCTGTGCCC
AGGACTCCGCTGGCAGGCCCCAATATTGACTACCCCGTGCTCTACGGAAA
GTACTTAAACGGACTGGGACGGTTGCCCGCCAAGACCCTCAAGCCAGAAG
TCCGCCTGGTGAAGCTGCCGTTCTTTAATATGCTGGATGAGCTGCTGAAG
CCCACCGAATTAGTCCCACAGAACAACGAGAAGCTTCAGGAGAGCCCGTG
CATCTTCGCATTGACGCCAAGACAGGTGGAGTTGATCCGGAACTCCAGGG
AACTGCAGCCCGGAGTTAAAGCCGTGCAGGTCGTCCTGAGAATCTGTTAC
TCAGACACCAGCTGCCCTCAGGAGGACCAGTACCCGCCCAACATCGCTGT
GAAGGTCAACCACAGCTACTGCTCCGTCCCGGGCTACTACCCCTCCAATA
AGCCCGGGGTGGAGCCCAAGAGGCCGTGCCGCCCCATCAACCTCACTCAC
CTCATGTACCTGTCCTCGGCCACCAACCGCATCACTGTCACCTGGGGGAA
CTACGGCAAGAGCTACTCGGTGGCCCTGTACCTGGTGCGGCAGCTGACCT
CATCGGAGCTGCTGCAGAGGCTGAAGACCATTGGGGTAAAGCACCCGGAG
CTGTGCAAGGCACTGGTCAAGGAGAAGCTGCGCCTTGATCCTGACAGCGA
GATCGCCACCACCGGTGTGCGGGTGTCCCTCATCTGTCCGCTGGTGAAGA
TGCGGCTCTCCGTGCCCTGCCGGGCAGAGACCTGCGCCCACCTGCAGTGC
TTCGACGCCGTCTTCTACCTGCAGATGAACGAGAAGAAGCCCACCTGGAT
GTGCCCCGTGTGCGACAAGCCAGCCCCCTACGACCAGCTCATCATCGACG
GGCTCCTCTCGAAGATCCTGAGCGAGTGTGAGGACGCCGACGAGATCGAG
TACCTGGTGGACGGCTCGTGGTGCCCGATCCGCGCCGAAAAGGAGCGCAG
CTGCAGCCCGCAGGGCGCCATCCTCGTGCTGGGCCCCTCGGACGCCAATG
GGCTCCTGCCCGCCCCCAGCGTCAACGGGAGCGGTGCCCTGGGCAGCACG
GGTGGCGGCGGCCCGGTGGGCAGCATGGAGAATGGGAAGCCGGGCGCCGA
TGTGGTGGACCTCACGCTGGACAGCTCATCGTCCTCGGAGGATGAGGAGG
AGGAGGAAGAGGAGGAGGAAGACGAGGACGAAGAGGGGCCCCGGCCCAAG
CGCCGCTGCCCCTTCCAGAAGGGCCTGGTGCCGGCCTGCTGACCCCGGCC
GCACACTCGACgcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSKMAAELVEA
KNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKI
KELYETRYAKKNSEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDY
PVLYGKYLNGLGRLPAKTLKPEVRLVKLPFFNMLDELLKPTELVPQNNEK
LQESPCIFALTPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQY
PPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRI
TVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLR
LDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNE
KKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIR
AEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMEN
GKPGADVVDLTLDSSSSSEDEEEEEEEEEDEDEEGPRPKRRCPFQKGLVP
AC*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24517
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: constructed by Anne Seifert (Ron Hay Grp). Clone harbours an additional N-terminal lysine upstream from PIAS4 initiation. ORF stop located approx 20bp upstream of 3' Not1 cloning site.