cDNA Clone IAA17 (A. thaliana)

• Plasmid: pET15 6His 3C 3X mini degron (A. thaliana IAA17 65-132 repeats)
• Parent Plasmid: pET15b 6His SUMO
• Expressed: 6His 3C 3X mini degron (A. thaliana IAA17 65-132 repeats)
• Genbank: U49073.1 GI:2618722
• Tag: 6His
• Species: Arabidopsis thaliana

Sequence of Insert

GGATCCCGTACGCTGCAGGTCGACGGTGCCGGTGCTGGTGCTGGTGCTGGTGCCGGTAAAGAAAAGAGTGCTTGCCCAAAAGACCCTGCTAAACCTCCTGCCAAGGCTCAAGTTGTAGGATGGCCACCTGTTAGATCATACAGAAAGAATGTCATGGTTTCTTGTCAAAAGTCTTCAGGTGGACCAGAAGCTGCAGCCTTTGTTAAAGTATCTATGGATGGTGCCCCATATTTGAGAAAGATTGACTTAAGGATGTACAAGGGTGCTGGAGCAGGCGCCAAAGAAAAATCTGCCTGTCCAAAAGATCCTGCAAAGCCACCTGCTAAAGCACAAGTCGTGGGTTGGCCTCCTGTTAGAAGTTATAGAAAGAATGTGATGGTTTCTTGCCAAAAATCTAGTGGCGGTCCTGAAGCCGCAGCCTTCGTCAAAGTATCTATGGACGGCGCTCCTTATTTGCGTAAGATAGACTTGCGTATGTATAAAGGTGCCGGAGCTGGCGCAGGTGCTAAGGAAAAATCTGCGTGTCCAAAGGACCCTGCAAAACCACCAGCCAAGGCACAAGTTGTAGGTTGGCCCCCTGTAAGATCCTATAGAAAGAATGTTATGGTTTCTTGCCAAAAATCTTCTGGAGGCCCTGAAGCAGCTGCATTTGTTAAAGTTAGTATGGACGGTGCTCCTTACTTGAGAAAAATAGACTTGAGAATGTATAAATGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSRTLQVDGAGAGAGAGAGKEKSACPKDP
AKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVKVSMDGAPYLR
KIDLRMYKGAGAGAKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSC
QKSSGGPEAAAFVKVSMDGAPYLRKIDLRMYKGAGAGAGAKEKSACPKDP
AKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVKVSMDGAPYLR
KIDLRMYK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU49788
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee