cDNA Clone C15orf41/CDAN1

• Synonyms: CDA1; CDAI; CDAN1A; DLT; PRO1295
• Plasmid: pFBDM untagged C15orf41 D196A/FLAG 6His TEV CDAN1
• Parent Plasmid: pFBDM
• Expressed: FLAG 6His TEV CDAN1
• Genbank: NM_138477.2 GI:57222569 with residue difference S421F (present in many published sequences for CDAN1)
• Tag: FLAG
• Tag: 6His
• Species: Human

Sequence of Insert

CTCGAGATGGACTACAAGGACGACGATGACAAGTCGTACTACCATCACCA
TCACCATCACGATTACGATATCCCAACGACCGAAAACCTGTATTTTCAGG
GCGCCATGGGATCCATGGCGGCCGTTTTGGAGTCGCTGCTGCGAGAAGAG
GTGTCGGTCGCAGCCGTCGTGCGGTGGATCGCGCGCAGCACCCAGGGTTC
GGAGGATAACGCTGGGGAGGCGGCCGCGCTGAGCTCACTCCGGGCCCTGC
GGAAAGAATTCGTACCGTTCCTGTTGAACTTCCTGAGGGAGCAGAGCAGC
CGCGTCCTCCCGCAGGGGCCCCCGACCCCCGCCAAGACCCCGGGCGCCTC
GGCAGCCTTGCCAGGGAGGCCGGGAGGCCCGCCGCGGGGTAGCCGCGGGG
CGCGCAGCCAGCTTTTCCCTCCGACCGAGGCCCAGAGCACCGCTGCCGAG
GCCCCTCTGGCCCGCCGCGGGGGCAGGAGGCGGGGCCCGGGGCCGGCCCG
CGAGCGTGGAGGCCGCGGCCTGGAGGAGGGGGTCAGCGGGGAGAGCCTGC
CCGGAGCCGGGGGCCGGAGGCTTAGGGGCTCTGGCAGCCCCAGCCGCCCC
AGCCTCACGCTGTCTGATCCGCCAAACCTCAGCAACCTGGAGGAGTTCCC
TCCCGTAGGCTCGGTTCCCCCCGGCCCTACAGGGACGAAGCCTTCTCGCA
GGATCAACCCAACTCCGGTGAGCGAAGAGCGGTCACTCTCCAAGCCCAAG
ACCTGCTTCACCTCACCCCCAATCAGCTGTGTCCCCAGTTCCCAACCCTC
AGCCCTGGACACTAGCCCTTGGGGCCTTGGCCTTCCCCCAGGGTGCAGAA
GTCTGCAAGAGGAGCGAGAGATGCTCAGGAAGGAGCGCTCTAAGCAGCTG
CAGCAGTCACCTACCCCCACCTGTCCCACCCCAGAATTGGGGTCGCCCCT
CCCCAGCCGGACAGGAAGCCTCACAGATGAACCTGCAGACCCTGCCAGAG
TGTCTTCCCGCCAGCGCCTGGAGCTTGTAGCCCTTGTTTACTCCTCGTGC
ATTGCTGAGAACCTGGTACCAAACCTCTTCTTGGAGCTTTTCTTCGTCTT
TCAGCTCCTCACTGCCCGGAGGATGGTGACTGCCAAGGACAGCGACCCTG
AACTAAGTCCAGCTGTCCTAGATTCCCTGGAAAGTCCACTGTTCCAAAGC
ATCCACGATTGTGTCTTCTTTGCAGTGCAGGTTTTGGAGTGTCACTTTCA
GGTTCTTTCCAACCTGGACAAAGGGACCTTGAAGCTGCTGGCTGAGAATG
AGCGGCTGCTGTGCTTCTCACCAGCTCTGCAAGGCCGCCTTCGAGCTGCC
TATGAGGGCAGTGTTGCCAAGGTCTTTCTGGTGATGCCTCCCTCTACTCA
AGCTGTCTCCTTTCAGCCAGAGACTGACAATCGTGCCAACTTCTCCAGTG
ACCGAGCCTTTCATACTTTTAAGAAACAGAGGGATGTGTTTTATGAGGTG
CTGCGAGAGTGGGAAGATCACCATGAGGAGCCTGGCTGGGATTTTGAGAA
GGGCTTGGGCAGCAGAATCAGGGCCATGATGGGTCAGCTCTCCGCAGCCT
GCAGCCACAGCCACTTTGTTCGGCTTTTCCAAAAACAACTACTCCAGATG
TGTCAGAGCCCTGGTGGTGCTGGGGGCACCGTCTTGGGCGAGGCCCCAGA
TGTGTTGAGTATGCTGGGAGCTGACAAGCTGGGGCGGTTGTGGCGCCTAC
AGGAACGGCTTATGGCTCCTCAGAGCAGTGGGGGGCCCTGCCCACCCCCC
ACCTTCCCAGGCTGTCAAGGCTTCTTTAGGGACTTCATCCTTAGTGCCAG
CAGCTTCCAGTTTAACCAGCATCTCATGGACAGTCTGAGCTTGAAGATCC
AGGAGCTCAATGGTCTTGCCCTGCCCCAGCATGAGCCCAATGATGAAGAC
GGGGAGTCAGACGTAGACTGGCAGGGTGAGCGGAAGCAATTTGCTGTGGT
GCTTCTTAGCCTGAGACTTTTGGCTAAATTCCTGGGCTTTGTGGCTTTCC
TGCCATACCGGGGGCCTGAACCTCCCCCGACCGGTGAGCTTCAGGACTCC
ATTCTGGCCCTCAGGAGCCAGGTCCCTCCGGTCCTGGATGTGCGGACTCT
GCTGCAGCGAGGGCTGCAGGCCCGCCGGGCGGTGCTCACCGTGCCCTGGC
TGGTGGAGTTTCTCTCCTTTGCTGACCATGTTGTTCCCTTGCTGGAATAT
TACCGGGACATCTTCACTCTCCTGCTGCGCCTGCACCGGAGCTTGGTGTT
GTCGCAGGAGAGTGAGGGGAAGATGTGTTTCCTGAACAAGCTGCTGCTAC
TTGCTGTCCTGGGCTGGCTTTTCCAGATTCCCACAGTCCCTGAGGACTTG
TTCTTTCTGGAAGAGGGTCCCTCATATGCCTTTGAGGTGGACACAGTAGC
CCCAGAGCATGGCTTGGACAATGCGCCTGTGGTGGACCAGCAGCTGCTCT
ACACCTGCTGCCCCTACATCGGAGAGCTCCGGAAACTGCTCGCTTCGTGG
GTGTCAGGCAGTAGTGGACGGAGTGGGGGCTTCATGAGGAAAATCACCCC
CACCACTACCACCAGCCTGGGAGCCCAGCCTTCCCAGACCAGCCAGGGGC
TGCAGGCACAGCTCGCCCAGGCCTTTTTCCACAACCAGCCGCCCTCCTTG
CGCCGGACCGTAGAGTTCGTGGCAGAAAGAATTGGATCAAACTGTGTCAA
ACATATCAAGGCTACACTGGTGGCAGATCTGGTGCGCCAGGCAGAGTCAC
TTCTCCAAGAGCAGCTGGTGACACAGGGAGAGGAAGGGGGAGACCCAGCC
CAGCTGTTGGAGATCTTGTGTTCCCAGCTGTGCCCTCACGGGGCCCAGGC
ATTGGCCCTGGGGCGGGAGTTCTGTCAAAGGAAGAGCCCTGGGGCTGTGC
GGGCGCTGCTTCCAGAGGAGACCCCGGCAGCCGTTCTGAGCAGTGCAGAG
AACATTGCTGTGGGGCTTGCAACAGAGAAAGCCTGTGCTTGGCTGTCAGC
CAACATCACAGCACTGATCAGGAGGGAGGTGAAAGCAGCAGTGAGTCGCA
CACTTCGAGCCCAGGGTCCTGAACCTGCTGCCCGGGGGGAGCGGAGGGGC
TGCTCCCGCGCCTGTGAGCACCATGCTCCCCTCCCCTCCCACCTCATCTC
CGAGATAAAAGACGTGCTCTCCTTGGCCGTGGGGCCACGGGACCCTGACG
AGGGAGTCTCCCCAGAGCATCTGGAACAGCTCCTAGGCCAGCTGGGCCAG
ACGCTGCGGTGCCGCCAGTTCCTGTGCCCACCTGCTGAGCAGCATCTGGC
AAAGTGCTCTGTGGAGTTAGCTTCCCTCCTCGTTGCAGATCAAATTCCTA
TCCTAGGGCCCCCGGCACAGTACAGGCTGGAGAGAGGGCAGGCTCGAAGG
CTTCTGCACATGCTGCTTTCCTTGTGGAAGGAAGACTTTCAGGGGCCGGT
TCCGCTGCAGCTGCTGCTGAGCCCAAGAAATGTGGGGCTTCTGGCAGACA
CAAGGCCAAGGGAGTGGGACTTGCTGCTATTCTTGCTACGGGAGCTGGTG
GAGAAGGGTCTGATGGGACGGATGGAGATAGAGGCCTGCCTGGGCAGCCT
CCACCAGGCCCAGTGGCCAGGGGACTTTGCTGAAGAATTAGCAACACTGT
CTAATCTGTTTCTAGCCGAGCCCCACCTGCCAGAACCCCAGCTAAGAGCC
TGTGAGTTGGTGCAGCCAAACCGGGGCACTGTGCTGGCCCAGAGCTAGAT
GCAT

Amino Acid Sequence

MDYKDDDDKSYYHHHHHHDYDIPTTENLYFQGAMGSMAAVLESLLREEVS
VAAVVRWIARSTQGSEDNAGEAAALSSLRALRKEFVPFLLNFLREQSSRV
LPQGPPTPAKTPGASAALPGRPGGPPRGSRGARSQLFPPTEAQSTAAEAP
LARRGGRRRGPGPARERGGRGLEEGVSGESLPGAGGRRLRGSGSPSRPSL
TLSDPPNLSNLEEFPPVGSVPPGPTGTKPSRRINPTPVSEERSLSKPKTC
FTSPPISCVPSSQPSALDTSPWGLGLPPGCRSLQEEREMLRKERSKQLQQ
SPTPTCPTPELGSPLPSRTGSLTDEPADPARVSSRQRLELVALVYSSCIA
ENLVPNLFLELFFVFQLLTARRMVTAKDSDPELSPAVLDSLESPLFQSIH
DCVFFAVQVLECHFQVLSNLDKGTLKLLAENERLLCFSPALQGRLRAAYE
GSVAKVFLVMPPSTQAVSFQPETDNRANFSSDRAFHTFKKQRDVFYEVLR
EWEDHHEEPGWDFEKGLGSRIRAMMGQLSAACSHSHFVRLFQKQLLQMCQ
SPGGAGGTVLGEAPDVLSMLGADKLGRLWRLQERLMAPQSSGGPCPPPTF
PGCQGFFRDFILSASSFQFNQHLMDSLSLKIQELNGLALPQHEPNDEDGE
SDVDWQGERKQFAVVLLSLRLLAKFLGFVAFLPYRGPEPPPTGELQDSIL
ALRSQVPPVLDVRTLLQRGLQARRAVLTVPWLVEFLSFADHVVPLLEYYR
DIFTLLLRLHRSLVLSQESEGKMCFLNKLLLLAVLGWLFQIPTVPEDLFF
LEEGPSYAFEVDTVAPEHGLDNAPVVDQQLLYTCCPYIGELRKLLASWVS
GSSGRSGGFMRKITPTTTTSLGAQPSQTSQGLQAQLAQAFFHNQPPSLRR
TVEFVAERIGSNCVKHIKATLVADLVRQAESLLQEQLVTQGEEGGDPAQL
LEILCSQLCPHGAQALALGREFCQRKSPGAVRALLPEETPAAVLSSAENI
AVGLATEKACAWLSANITALIRREVKAAVSRTLRAQGPEPAARGERRGCS
RACEHHAPLPSHLISEIKDVLSLAVGPRDPDEGVSPEHLEQLLGQLGQTL
RCRQFLCPPAEQHLAKCSVELASLLVADQIPILGPPAQYRLERGQARRLL
HMLLSLWKEDFQGPVPLQLLLSPRNVGLLADTRPREWDLLLFLLRELVEK
GLMGRMEIEACLGSLHQAQWPGDFAEELATLSNLFLAEPHLPEPQLRACE
LVQPNRGTVLAQS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU49795
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: FLAG 6His TEV CDAN1 subcloned from DU49765 into DU49703 XhoI/NsiI