cDNA Clone VWA8

• Synonyms: KIAA0564
• Plasmid: pcDNA5D FRT/TO VWA8-GFP
• Parent Plasmid: pcDNA5D FRT/TO C-GFP
• Expressed: VWA8-GFP
• Genbank: NM_015058.1 GI:57863270 with silent changes t1941c and t3642c
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGCCACCATGCAATCCCGGCTTCTACTCCTCGGGGCACCCGGAGGCCACGGCGGCCCGGCCTCGCGGCGCATGCGGCTGCTCCTGCGGCAGGTGGTGCAGCGCAGGCCGGGTGGCGACAGGCAGCGGCCGGAGGTCAGACTGTTGCACGCCGGCTCGGGGGCCGACACAGGTGATACAGTTAATATTGGAGATGTATCCTACAAGTTGAAAATTCCTAAGAATCCAGAACTTGTGCCACAGAACTACATTTCAGACTCTCTGGCTCAATCTGTAGTTCAGCATCTAAGATGGATAATGCAGAAGGATCTTTTGGGGCAAGATGTTTTTCTAATAGGACCTCCTGGGCCTCTTCGACGCTCTATTGCTATGCAGTACTTGGAGCTGACCAAACGGGAGGTCGAATACATTGCCCTGTCAAGGGACACCACTGAAACTGATCTCAAACAGCGACGAGAGATCCGTGCAGGCACAGCCTTTTACATTGATCAGTGTGCAGTTCGTGCAGCCACAGAAGGCAGAACTCTCATTTTGGAAGGTTTGGAAAAGGCAGAGAGGAATGTTTTGCCTGTTTTGAACAACTTGCTGGAAAACAGAGAGATGCAGCTTGAAGATGGACGCTTCCTGATGTCTGCTGAGCGTTACGACAAACTTCTCCGAGATCATACCAAAAAAGAGTTGGATTCTTGGAAAATTGTCCGAGTTAGTGAAAATTTCCGAGTGATTGCCTTGGGCTTGCCAGTGCCAAGGTATTCTGGGAATCCATTAGACCCCCCTCTTCGTTCTCGATTTCAAGCCAGGGATATTTATTATTTACCCTTCAAGGACCAACTTAAGTTGTTATATTCAATTGGAGCCAATGTTTCTGCTGAGAAAGTTTCTCAGCTCTTGTCCTTTGCCACAACTCTGTGTTCCCAAGAATCTTCTACTCTTGGACTTCCAGACTTTCCTTTAGATAGTTTAGCAGCTGCGGTTCAAATCTTGGATTCCTTTCCTATGATGCCAATCAAACATGCAATCCAGTGGCTTTATCCATATAGTATTTTACTAGGTCATGAAGGGAAGATGGCTGTGGAAGGTGTTTTAAAGCGCTTTGAACTTCAAGATTCAGGAAGCTCTCTACTTCCTAAAGAGATTGTAAAAGTAGAGAAGATGATGGAAAACCATGTGTCCCAAGCTTCTGTGACCATCCGGATTGCAGATAAAGAGGTGACCATTAAGGTGCCAGCCGGGACCAGGCTATTAAGTCAACCTTGTGCGTCAGACCGTTTCATACAGACTTTGAGCCATAAGCAGCTACAGGCTGAAATGATGCAGTCTCACATGGTTAAAGATATATGTTTAATTGGAGGAAAGGGTTGTGGAAAAACAGTGATCGCTAAGAACTTTGCCGATACCTTAGGATACAACATAGAACCTATTATGCTCTATCAGGATATGACAGCGCGTGATCTGCTACAGCAGAGATACACCCTTCCAAATGGAGACACTGCCTGGCGGTCCTCACCCCTTGTGAATGCTGCTCTGGAAGGCAAGCTGGTCCTGCTGGATGGCATTCACCGGGTGAATGCGGGCACGCTTGCTGTATTGCAAAGGTTAATCCATGATCGAGAGCTAAGCCTCTATGATGGTTCTAGGCTGCTGAGAGAAGACAGGTATATGCGTTTAAAGGAGGAGCTGCAACTGTCTGATGAACAGCTACAGAAGAGATCCATTTTTCCTATCCATCCCTCCTTCAGAATCATTGCCTTGGCAGAACCCCCTGTTATTGGAAGCACAGCACACCAGTGGCTGGGACCAGAATTCTTAACCATGTTCTTTTTCCATTACATGAAACCACTTGTGAAAAGTGAAGAAATCCAAGTGATTAAGGAAAAGGTCCCAAATGTACCTCAGGAAGCTCTGGATAAGTTATTATCATTTACACACAAACTCAGAGAAACACAGGACCCAACGGCACAATCATTAGCGGCATCACTTTCTACCAGACAACTGTTGCGAATTTCTCGTCGGCTGTCACAGTATCCTAATGAAAATCTTCACAGTGCTGTTACTAAAGCCTGCCTTTCCAGGTTTTTACCCAGTCTTGCTAGGTCAGCATTAGAAAAAAATCTGGCAGATGCTACAATAGAAATAAATACTGATGACAATTTGGAGCCAGAACTGAAGGATTACAAATGTGAAGTAACATCTGGAACTCTGAGGATTGGTGCTGTTAGTGCACCGATCTATAATGCACATGAGAAAATGAAAGTGCCTGATGTTCTTTTCTATGACAACATTCAGCATGTGATAGTGATGGAAGATATGCTGAAAGACTTTCTCCTTGGAGAACACTTATTATTGGTTGGCAACCAGGGTGTAGGAAAAAACAAGATTGTTGACAGATTCCTTCACCTGCTCAACAGACCCCGAGAATATATTCAGCTACACAGGGATACCACAGTACAAACTCTTACGCTTCAGCCTTCGGTTAAAGACGGACTTATTGTATATGAAGACTCACCTTTGGTTAAAGCAGTAAAGTTGGGTCATATTCTGGTAGTAGATGAGGCTGACAAAGCTCCAACAAATGTCACGTGTATTTTAAAAACTCTAGTAGAAAATGGAGAAATGATTCTAGCAGATGGAAGACGCATTGTTGCAAATTCTGCTAATGTGAATGGAAGAGAAAATGTTGTAGTGATTCATCCTGATTTTAGGATGATTGTTCTGGCAAATAGACCTGGATTTCCTTTCCTAGGCAATGATTTCTTCGGTACCTTAGGTGATATTTTTAGCTGCCATGCAGTTGATAACCCCAAACCCCACTCGGAGCTCGAGATGCTCAGACAGTATGGACCAAATGTGCCTGAGCCCATCCTTCAGAAGCTTGTGGCTGCCTTTGGAGAGCTGAGGAGTTTGGCTGACCAAGGGATTATTAACTATCCTTATTCTACCAGAGAAGTTGTCAACATAGTCAAACATTTACAGAAATTTCCGACTGAAGGTCTCTCCAGTGTAGTTCGAAATGTGTTTGACTTTGATTCCTACAACAATGACATGAGGGAGATATTGATTAACACTTTACACAAATACGGGATACCTATCGGAGCAAAGCCTACCAGTGTGCAGCTGGCAAAGGAGTTGACTCTGCCAGAACAAACGTTCATGGGCTACTGGACAATTGGTCAGGCAAGAAGTGGGATGCAAAAACTCTTGTGTCCAGTGGAAACTCATCATATAGACATAAAGGGTCCAGCACTTATAAATATACAGGAGTATCCAATAGAAAGACATGAAGAAAGATCCCTAAACTTTACTGAAGAATGTGCCTCATGGAGAATACCATTGGATGAAATTAATATAATCTGTGACATTGCTACATCACATGAAAATGAGCAAAATACTCTCTATGTAGTTACATGCAATCCCGCTTCCCTGTACTTTATGAATATGACTGGGAAAAGTGGCTTCTTTGTGGACTTTTTTGATATCTTCCCAAGAACAGCCAATGGCGTTTGGCACCCTTTTGTGACAGTGGCACCGCTGGGAAGTCCTCTCAAAGGTCAAGTGGTTCTCCATGAGCAGCAGAGTAATGTTATCCTGTTGTTAGATACTACTGGCCGGGCCCTTCATCGTCTCATCCTCCCTTCCGAGAAGTTTACATCCAAGAAACCTTTCTGGTGGAACAAAGAAGAAGCTGAAACTTATAAAATGTGTAAAGAATTTTCACACAAAAACTGGCTGGTGTTCTACAAAGAAAAAGGGAACAGCCTGACTGTGCTGGATGTTCTAGAAGGGCGAACTCACACCATCTCACTTCCCATCAACCTCAAGACAGTTTTCCTTGTAGCAGAGGACAAATGGCTTCTGGTGGAGAGCAAAACAAATCAGAAATATCTTTTAACTAAGCCTGCACACATCGAATCTGAGGGTAGTGGGGTTTGCCAGTTGTATGTGCTGAAAGAGGAGCCGCCCAGCACAGGGTTTGGAGTTACACAAGAAACAGAGTTCAGCATACCTCATAAAATTTCCAGTGATCAACTATCATCTGAACATCTAAGTTCAGCTGTGGAACAAAAGATTGCCTCTCCCAACAGAATTCTCTCAGATGAGAAAAATTATGCTACAATAGTTGTTGGTTTTCCAGATCTCATGTCACCCAGTGAAGTTTATTCTTGGAAGAGACCATCATCTTTGCATAAACGAAGTGGCACTGATACATCATTCTATAGAGGAAAGAAGAAAAGGGGGACTCCAAAACAAAGCAATTGTGTGACTCTTTTAGATACGAATCAGGTAGTGAGGATTTTACCCCCAGGAGAAGTCCCTCTAAAAGATATCTACCCAAAAGATGTTACTCCTCCACAAACATCTGGTTATATAGAAGTCACTGATCTTCAATCAAAGAAACTCCGATATATCCCTATTCCCAGATCAGAATCTCTCTCGCCGTATACCACATGGCTGTCGACCATTTCAGACACAGATGCACTGCTGGCTGAGTGGGACAAAAGCGGTGTTGTTACTGTTGATATGGGAGGTCACATCAGGCTTTGGGAAACTGGACTTGAACGTCTGCAGCGATCACTCATGGAATGGAGAAACATGATTGGACAAGATGACAGAAATATGCAGATAACAATCAACAGAGACAGTGGTGAAGATGTAAGCTCCCCCAAACACGGGAAGGAGGACCCAGACAACATGCCTCACGTGGGCGGCAACACTTGGGCTGGCGGAACAGGGGGAAGAGACACGGCAGGCCTGGGTGGCAAAGGAGGCCCTTACCGGCTGGATGCAGGCCATACGGTGTACCAGGTCTCTCAGGCTGAGAAAGATGCAGTTCCCGAAGAGGTCAAGAGAGCTGCTAGAGAGATGGGCCAGAGAGCATTCCAGCAGAGGCTAAAGGAGATCCAAATGAGTGAATACGATGCTGCAACCTATGAAAGGTTTTCAGGTGCTGTTCGGCGACAGGTGCACTCCCTCCGAATCATCCTGGATAATTTACAGGCTAAAGGTAAAGAAAGACAATGGCTAAGACATCAAGCTACTGGAGAATTAGATGATGCCAAGATCATTGATGGGCTGACTGGAGAAAAAGCCATCTACAAACGTCGGGGTGAGCTGGAGCCACAACTTGGCAGCCCACAACAGAAACCCAAGCGTCTGCGCCTGGTGGTAGATGTGTCTGGTAGCATGTACCGTTTCAACAGGATGGATGGCCGGCTTGAGCGCACAATGGAGGCTGTGTGTATGGTCATGGAAGCCTTCGAGAACTATGAGGAGAAGTTCCAGTATGACATCGTTGGACACTCTGGAGATGGCTACAACATTGGTCTGGTTCCAATGAACAAAATCCCCAAGGACAATAAGCAAAGACTAGAAATTCTGAAGACAATGCATGCCCACTCTCAGTTCTGCATGAGTGGGGACCACACGTTAGAAGGGACAGAACATGCCATCAAGGAAATTGTCAAAGAAGAAGCTGATGAGTACTTTGTCATAGTCTTGAGTGATGCAAATCTGTCACGATATGGAATACATCCTGCTAAGTTTGCTCAAATCCTCACAAGAGACCCTCAAGTAAATGCTTTTGCCATTTTTATTGGCTCTTTAGGTGATCAAGCAACCAGGCTTCAGAGAACTTTACCAGCTGGTCGGTCTTTCGTTGCCATGGATACCAAGGATATCCCTCAGATTTTACAACAGATCTTCACCTCCACCATGTTGTCGAGTGTCGCGGCCGC

Amino Acid Sequence

MQSRLLLLGAPGGHGGPASRRMRLLLRQVVQRRPGGDRQRPEVRLLHAGS
GADTGDTVNIGDVSYKLKIPKNPELVPQNYISDSLAQSVVQHLRWIMQKD
LLGQDVFLIGPPGPLRRSIAMQYLELTKREVEYIALSRDTTETDLKQRRE
IRAGTAFYIDQCAVRAATEGRTLILEGLEKAERNVLPVLNNLLENREMQL
EDGRFLMSAERYDKLLRDHTKKELDSWKIVRVSENFRVIALGLPVPRYSG
NPLDPPLRSRFQARDIYYLPFKDQLKLLYSIGANVSAEKVSQLLSFATTL
CSQESSTLGLPDFPLDSLAAAVQILDSFPMMPIKHAIQWLYPYSILLGHE
GKMAVEGVLKRFELQDSGSSLLPKEIVKVEKMMENHVSQASVTIRIADKE
VTIKVPAGTRLLSQPCASDRFIQTLSHKQLQAEMMQSHMVKDICLIGGKG
CGKTVIAKNFADTLGYNIEPIMLYQDMTARDLLQQRYTLPNGDTAWRSSP
LVNAALEGKLVLLDGIHRVNAGTLAVLQRLIHDRELSLYDGSRLLREDRY
MRLKEELQLSDEQLQKRSIFPIHPSFRIIALAEPPVIGSTAHQWLGPEFL
TMFFFHYMKPLVKSEEIQVIKEKVPNVPQEALDKLLSFTHKLRETQDPTA
QSLAASLSTRQLLRISRRLSQYPNENLHSAVTKACLSRFLPSLARSALEK
NLADATIEINTDDNLEPELKDYKCEVTSGTLRIGAVSAPIYNAHEKMKVP
DVLFYDNIQHVIVMEDMLKDFLLGEHLLLVGNQGVGKNKIVDRFLHLLNR
PREYIQLHRDTTVQTLTLQPSVKDGLIVYEDSPLVKAVKLGHILVVDEAD
KAPTNVTCILKTLVENGEMILADGRRIVANSANVNGRENVVVIHPDFRMI
VLANRPGFPFLGNDFFGTLGDIFSCHAVDNPKPHSELEMLRQYGPNVPEP
ILQKLVAAFGELRSLADQGIINYPYSTREVVNIVKHLQKFPTEGLSSVVR
NVFDFDSYNNDMREILINTLHKYGIPIGAKPTSVQLAKELTLPEQTFMGY
WTIGQARSGMQKLLCPVETHHIDIKGPALINIQEYPIERHEERSLNFTEE
CASWRIPLDEINIICDIATSHENEQNTLYVVTCNPASLYFMNMTGKSGFF
VDFFDIFPRTANGVWHPFVTVAPLGSPLKGQVVLHEQQSNVILLLDTTGR
ALHRLILPSEKFTSKKPFWWNKEEAETYKMCKEFSHKNWLVFYKEKGNSL
TVLDVLEGRTHTISLPINLKTVFLVAEDKWLLVESKTNQKYLLTKPAHIE
SEGSGVCQLYVLKEEPPSTGFGVTQETEFSIPHKISSDQLSSEHLSSAVE
QKIASPNRILSDEKNYATIVVGFPDLMSPSEVYSWKRPSSLHKRSGTDTS
FYRGKKKRGTPKQSNCVTLLDTNQVVRILPPGEVPLKDIYPKDVTPPQTS
GYIEVTDLQSKKLRYIPIPRSESLSPYTTWLSTISDTDALLAEWDKSGVV
TVDMGGHIRLWETGLERLQRSLMEWRNMIGQDDRNMQITINRDSGEDVSS
PKHGKEDPDNMPHVGGNTWAGGTGGRDTAGLGGKGGPYRLDAGHTVYQVS
QAEKDAVPEEVKRAAREMGQRAFQQRLKEIQMSEYDAATYERFSGAVRRQ
VHSLRIILDNLQAKGKERQWLRHQATGELDDAKIIDGLTGEKAIYKRRGE
LEPQLGSPQQKPKRLRLVVDVSGSMYRFNRMDGRLERTMEAVCMVMEAFE
NYEEKFQYDIVGHSGDGYNIGLVPMNKIPKDNKQRLEILKTMHAHSQFCM
SGDHTLEGTEHAIKEIVKEEADEYFVIVLSDANLSRYGIHPAKFAQILTR
DPQVNAFAIFIGSLGDQATRLQRTLPAGRSFVAMDTKDIPQILQQIFTST
MLSSVAAAMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGK
LTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEG
YVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKL
EYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDG
PVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGL
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49915
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Recommend propagation at 30 degrees.