cDNA Clone INPP5F

• Synonyms: SAC2; hSAC2; MSTP007; MSTPO47
• Plasmid: pET15b 6HIS SUMO INPP5F
• Parent Plasmid: pET15b 6His SUMO
• Expressed: 6HIS SUMO INPP5F
• Genbank: NM_014937.3
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

ggatccATGGAGCTCTTCCAAGCCAAGGACCACTACATCCTGCAGCAGGGCGAGCGCGCGCTGTGGTGCAGCCGCCGCGACGGCGGCCTCCAGCTCCGACCCGCTACTGATCTACTTCTTGCCTGGAATCCCATTTGTTTGGGGTTGGTAGAAGGTGTTATTGGGAAAATTCAACTTCATTCAGATCTTCCATGGTGGCTTATTCTAATTCGGCAGAAAGCATTGGTGGGCAAACTCCCAGGAGACCATGAGGTCTGTAAAGTTACCAAAATTGCTGTGCTCTCACTTTCTGAAATGGAACCTCAGGATCTTGAGCTAGAGCTCTGTAAGAAGCATCATTTTGGTATTAACAAACCAGAGAAGATCATACCATCTCCTGATGACTCAAAGTTTCTACTGAAGACCTTTACGCATATTAAATCCAATGTGTCTGCTCCTAATAAAAAGAAAGTTAAGGAAAGTAAAGAGAAGGAGAAGTTGGAGAGGAGATTACTTGAAGAGTTGCTGAAGATGTTCATGGACTCAGAATCCTTTTATTATAGCTTGACCTATGACCTGACCAATTCCGTGCAGAGGCAGAGCACTGGGGAGAGGGACGGTCGGCCCCTCTGGCAGAAGGTTGATGACCGATTTTTTTGGAATAAATACATGATACAAGATCTTACTGAGATTGGTACTCCAGATGTGGACTTTTGGATTATCCCCATGATCCAAGGTTTTGTGCAGATTGAAGAACTTGTGGTTAATTATACCGAATCATCTGATGATGAGAAAAGCAGCCCAGAGACCCCCCCTCAGGAGTCCACCTGTGTAGATGATATTCACCCACGATTTCTAGTGGCTCTCATTTCACGCCGAAGTAGGCACAGAGCAGGAATGCGCTATAAACGAAGAGGAGTGGATAAAAATGGAAATGTTGCCAATTATGTGGAGACTGAGCAGTTGATTCATGTTCATAATCATACCCTGTCATTTGTTCAAACACGAGGCTCTGTGCCTGTCTTTTGGAGCCAGGTTGGGTATCGATATAACCCAAGACCGCGGCTGGACAGAAGTGAAAAGGAAACTGTTGCCTATTTCTGTGCCCATTTCGAAGAACAACTGAACATTTACAAAAAACAGGTTATTATTAACTTGGTAGACCAGGCAGGAAGAGAGAAGATTATTGGCGATGCTTACCTGAAGCAAGTGTTGCTTTTCAACAACTCACACCTCACTTACGTTTCGTTTGACTTCCATGAGCACTGCCGAGGAATGAAGTTTGAGAATGTTCAGACACTAACAGATGCCATTTATGACATTATTCTTGATATGAAGTGGTGTTGGGTTGATGAAGCTGGGGTAATATGTAAGCAGGAAGGGATTTTTCGTGTTAATTGTATGGACTGCCTGGATCGCACCAACGTGGTCCAAGCTGCCATCGCGAGAGTGGTCATGGAACAGCAGCTGAAAAAATTAGGTGTGATGCCCCCGGAACAGCCATTACCTGTGAAATGTAATCGCATCTACCAGATAATGTGGGCCAATAATGGTGACTCCATTAGCAGACAGTATGCTGGGACAGCTGCTCTGAAGGGTGACTTTACAAGGACAGGAGAAAGGAAGTTAGCAGGAGTTATGAAAGATGGAGTGAACTCAGCAAACAGATATTACCTCAACCGATTTAAGGATGCTTATAGGCAAGCTGTTATAGATTTGATGCAAGGCATTCCAGTGACAGAAGATCTTTATTCCATATTTACCAAGGAGAAAGAACATGAAGCTTTGCATAAGGAAAATCAGAGAAGCCACCAGGAACTAATTAGCCAGCTCTTACAAAGTTACATGAAGTTACTACTGCCTGATGATGAGAAGTTCCATGGGGGCTGGGCCCTCATTGACTGTGACCCTAGCCTCATTGATGCTACTCACAGAGACGTGGATGTGCTGTTACTGCTTTCTAACTCTGCCTACTACGTGGCCTATTATGATGATGAAGTTGATAAAGTAAACCAGTATCAACGACTAAGTCTAGAAAACCTGGAAAAAATTGAAATAGGCCCTGAACCCACTCTTTTTGGTAAGCCAAAGTTCTCCTGCATGCGACTGCACTACAGATACAAAGAAGCGAGTGGCTATTTCCACACATTGCGAGCTGTAATGCGTAATCCTGAAGAGGATGGAAAAGATACCCTTCAGTGCATTGCAGAGATGCTGCAGATCACCAAGCAAGCCATGGGATCGGATTTACCCATAATTGAGAAGAAACTTGAGAGGAAGAGCAGTAAACCTCACGAAGACATCATTGGTATCAGGTCTCAAAACCAAGGTTCTTTGGCCCAGGGAAAGAATTTTTTAATGAGCAAATTTTCATCTCTAAATCAAAAAGTGAAGCAGACCAAATCCAATGTAAATATTGGCAACCTCCGAAAGCTAGGAAACTTTACCAAACCTGAAATGAAAGTTAACTTTCTAAAACCAAACTTAAAAGTAAATCTTTGGAAATCAGATAGTAGTCTTGAAACTATGGAAAACACAGGAGTGATGGATAAGGTTCAGGCAGAGTCTGATGGGGACATGTCTTCAGATAATGACTCATACCACTCTGATGAATTCCTTACAAATTCTAAGTCTGATGAAGACAGGCAGCTAGCTAACTCATTAGAGAGTGTAGGGCCAATAGATTACGTTCTTCCTAGTTGTGGTATTATTGCCTCAGCGCCTCGATTGGGCAGTCGGTCCCAGTCTCTTAGCAGCACAGATAGTAGCGTTCATGCTCCTTCAGAGATTACTGTTGCTCATGGGAGTGGGCTTGGAAAAGGCCAGGAGTCTCCTTTGAAGAAAAGTCCTTCTGCTGGCGACGTACACATATTGACTGGCTTTGCCAAGCCTATGGATATTTACTGCCACAGATTTGTGCAAGATGCACAGAACAAAGTGACCCACCTATCAGAGACCAGATCTGTGTCTCAGCAGGCTAGTCAGGAAAGAAATCAAATGACCAATCAAGTTTCAAATGAAACCCAATCAGAATCAACAGAACAGACACCTTCTCGGCCATCGCAATTAGATGTCTCTCTTTCTGCAACAGGCCCACAGTTTTTGTCAGTTGAGCCAGCGCATTCAGTTGCATCTCAAAAAACCCCCACCTCCGCTTCCAGCATGCTTGAACTTGAGACAGGGCTTCATGTAACTCCTTCTCCTTCAGAGAGCAGTAGCAGCAGAGCAGTCTCTCCCTTTGCCAAGATTCGAAGTTCCATGGTCCAGGTTGCTAGTATTACCCAAGCTGGATTAACCCATGGGATAAACTTTGCAGTGTCAAAAGTTCAGAAGAGTCCTCCAGAACCTGAAATCATTAATCAAGTCCAGCAAAATGAACTTAAAAAGATGTTTATACAATGCCAGACACGGATAATTCAGATTTAGgcggccgc

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMELFQAKDHYILQQGERALWCSRRDGGLQLRPATDLLLAWNPICLGLVEGVIGKIQLHSDLPWWLILIRQKALVGKLPGDHEVCKVTKIAVLSLSEMEPQDLELELCKKHHFGINKPEKIIPSPDDSKFLLKTFTHIKSNVSAPNKKKVKESKEKEKLERRLLEELLKMFMDSESFYYSLTYDLTNSVQRQSTGERDGRPLWQKVDDRFFWNKYMIQDLTEIGTPDVDFWIIPMIQGFVQIEELVVNYTESSDDEKSSPETPPQESTCVDDIHPRFLVALISRRSRHRAGMRYKRRGVDKNGNVANYVETEQLIHVHNHTLSFVQTRGSVPVFWSQVGYRYNPRPRLDRSEKETVAYFCAHFEEQLNIYKKQVIINLVDQAGREKIIGDAYLKQVLLFNNSHLTYVSFDFHEHCRGMKFENVQTLTDAIYDIILDMKWCWVDEAGVICKQEGIFRVNCMDCLDRTNVVQAAIARVVMEQQLKKLGVMPPEQPLPVKCNRIYQIMWANNGDSISRQYAGTAALKGDFTRTGERKLAGVMKDGVNSANRYYLNRFKDAYRQAVIDLMQGIPVTEDLYSIFTKEKEHEALHKENQRSHQELISQLLQSYMKLLLPDDEKFHGGWALIDCDPSLIDATHRDVDVLLLLSNSAYYVAYYDDEVDKVNQYQRLSLENLEKIEIGPEPTLFGKPKFSCMRLHYRYKEASGYFHTLRAVMRNPEEDGKDTLQCIAEMLQITKQAMGSDLPIIEKKLERKSSKPHEDIIGIRSQNQGSLAQGKNFLMSKFSSLNQKVKQTKSNVNIGNLRKLGNFTKPEMKVNFLKPNLKVNLWKSDSSLETMENTGVMDKVQAESDGDMSSDNDSYHSDEFLTNSKSDEDRQLANSLESVGPIDYVLPSCGIIASAPRLGSRSQSLSSTDSSVHAPSEITVAHGSGLGKGQESPLKKSPSAGDVHILTGFAKPMDIYCHRFVQDAQNKVTHLSETRSVSQQASQERNQMTNQVSNETQSESTEQTPSRPSQLDVSLSATGPQFLSVEPAHSVASQKTPTSASSMLELETGLHVTPSPSESSSSRAVSPFAKIRSSMVQVASITQAGLTHGINFAVSKVQKSPPEPEIINQVQQNELKKMFIQCQTRIIQI*

• Vector Type: Bacterial
• Antibiotic: Amp; Kan
• DU Number: DU25812
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: linker after SENP cleavage deleted.