cDNA Clone WNK1

• Synonyms: HSAN2, HSN2, KDP, PRKWNK1, PSK, p65
• Plasmid: pcDNA5D frtTO FLAG WNK1 tv4 disease truncation
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: FLAG-WNK1 tv4 disease truncation
• Genbank: NM_018979.3
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCGCCATGTCTGGCGGCGCCGCAGAGAAGCAGAGCAGCACTCCCGGTTCCCTGTTCCTCTCGCCGCCGGCTCCTGCCCCCAAGAATGGCTCCAGCTCCGATTCCTCCGTGGGGGAGAAACTGGGAGCCGCGGCCGCCGACGCTGTGACCGGCAGGACCGAGGAGTACAGGCGCCGCCGCCACACTATGGACAAGGACAGCCGTGGGGCGGCCGCGACCACTACCACCACTGAGCACCGCTTCTTCCGCCGGAGCGTCATCTGCGACTCCAATGCCACTGCACTGGAGCTTCCCGGCCTTCCTCTTTCCCTGCCCCAGCCCAGCATCCCCGCGGCTGTCCCGCAGAGTGCTCCACCGGAGCCCCACCGGGAAGAGACCGTGACCGCCACCGCCACTTCCCAGGTAGCCCAGCAGCCTCCAGCCGCTGCCGCCCCTGGGGAACAGGCCGTCGCTGGCAGCGGCGGCGGCAGCGCCAAGGAGCCACAGGAGGAACGGAGCCAGCAGCAGGATGATATCGAAGAGCTGGAGACCAAGGCCGTGGGAATGTCTAACGATGGCCGCTTTCTCAAGTTTGACATCGAAATCGGCAGAGGCTCCTTTAAGACGGTCTACAAAGGTCTGGACACTGAAACCACCGTGGAAGTCGCCTGGTGTGAACTGCAGGATCGAAAATTAACAAAGTCTGAGAGGCAGAGATTTAAAGAAGAAGCTGAAATGTTAAAAGGTCTTCAGCATCCCAATATTGTTAGATTTTATGATTCCTGGGAATCCACAGTAAAAGGAAAGAAGTGCATTGTTTTGGTGACTGAACTTATGACGTCTGGAACACTTAAAACGTATCTGAAAAGGTTTAAAGTGATGAAGATCAAAGTTCTAAGAAGCTGGTGCCGTCAGATCCTTAAAGGTCTTCAGTTTCTTCATACTCGAACTCCACCTATCATTCACCGCGATCTTAAATGTGACAACATCTTTATCACCGGCCCTACTGGCTCAGTCAAGATTGGAGACCTCGGTCTGGCAACCCTGAAGCGGGCTTCTTTTGCCAAGAGTGTGATAGGTACCCCAGAGTTCATGGCCCCTGAGATGTATGAGGAGAAATATGATGAATCCGTTGACGTTTATGCTTTTGGGATGTGCATGCTTGAGATGGCTACATCTGAATATCCTTACTCGGAGTGCCAAAATGCTGCGCAGATCTACCGTCGCGTGACCAGTGGGGTGAAGCCAGCCAGTTTTGACAAAGTAGCAATTCCTGAAGTGAAGGAAATTATTGAAGGATGCATACGACAAAACAAAGATGAAAGATATTCCATCAAAGACCTTTTGAACCATGCCTTCTTCCAAGAGGAAACAGGAGTACGGGTAGAATTAGCAGAAGAAGATGATGGAGAAAAAATAGCCATAAAATTATGGCTACGTATTGAAGATATTAAGAAATTAAAGGGAAAATACAAAGATAATGAAGCTATTGAGTTTTCTTTTGATTTAGAGAGAGATGTCCCAGAAGATGTTGCACAAGAAATGGTAGAGTCTGGGTATGTCTGTGAAGGTGATCACAAGACCATGGCTAAAGCTATCAAAGACAGAGTATCATTAATTAAGAGGAAACGAGAGCAGCGGCAGTTGGTACGGGAGGAGCAAGAAAAAAAAAAGCAGGAAGAGAGCAGTCTCAAACAGCAGGTAGAACAATCCAGTGCTTCCCAGACAGGAATCAAGCAGCTCCCTTCTGCTAGCACCGGCATACCTACTGCTTCTACCACTTCAGCTTCAGTTTCTACACAAGTAGAACCTGAAGAACCTGAGGCAGATCAACATCAGCAACTACAGTACCAGCAACCCAGTATATCTGTGTTATCTGATGGGACGGTTGACAGTGGTCAGGGATCCTCTGTCTTCACAGAATCTCGAGTGAGCAGCCAACAGACAGTTTCATATGGTTCCCAACATGAACAGGCACATTCTACAGGCACAGTCCCAGGGCATATACCTTCTACTGTCCAAGCACAGTCTCAGCCCCATGGGGTATATCCACCCTCAAGTGTGCCTCAGTCCATGGCGCATCCGTGTGGGGGGACCCCAACATACCCAGAATCACAGATATTTTTCCCAACTATTCATGAACGTCCAGTTTCTTTTTCACCACCTCCCACCTGCCCACCGAAAGTAGCCATTTCCCAGCGGCGTAAGAGCACCTCCTTCCTGGAAGCCCAAACTCACCACTTCCAACCCCTGCTGAGGACTGTTGGCCAAAGTCTTCTTCCACCTGGTGGCAGCCCAACTAACTGGACACCAGAGGCCGTAGTTATGTTGGGTACTACAGCCAGTAGAGTAACTGGAGAGTCATGTGAGATACAGGTCCATCCTATGTTTGAACCATCTCAAGTTTACAGTGACTATAGACCTGGACTAGTACTTCCAGAAGAAGCTCACTATTTTATTCCTCAGGAAGCAGTGTATGTAGCTGGGGTACATTACCAGGCCCGGGTGGCAGAACAGTATGAGGGCATTCCATACAACTCATCAGTACTGTCAAGTCCTATGAAACAGATACCTGAACAGAAGCCAGTACAAGGGGGCCCTACTTCAAGTTCTGTCTTTGATTTCCATCTGGACAGGCTTTCCTGGTAGGTCGAC

Amino Acid Sequence

MDYKDDDDKGSPGEFAMSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSS
VGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSV
ICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVA
QQPPAAAAPGEQAVAGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDG
RFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEE
AEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKV
MKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGD
LGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATS
EYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERY
SIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKD
NEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRK
REQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTAST
TSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESR
VSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVPQSMA
HPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEA
QTHHFQPLLRTVGQSLLPPGGSPTNWTPEAVVMLGTTASRVTGESCEIQV
HPMFEPSQVYSDYRPGLVLPEEAHYFIPQEAVYVAGVHYQARVAEQYEGI
PYNSSVLSSPMKQIPEQKPVQGGPTSSSVFDFHLDRLSW*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48495
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Variant 4, designed to mimic a frameshift disease variant. Contains three silent nucleotide changes as compared to the reference sequence; T>C 258 bp, A>G 1287 bp and A>G 1914 bp. Grow at 30oC.