cDNA Clone DWNK

• Plasmid: pGEX6P-1 dWNK 214-716
• Parent Plasmid: pGEX6P1
• Expressed: GST-3C-dWNK 214-716
• Genbank: AGB94864
• Tag: GST
• Species: Drosophila Melanogaster

Sequence of Insert

GGATCCATGGAAGCACGTAAAAGCAGCAGCGATACCGTTATTGAACCGCTGATTAAACAGCAGGCACCGCTGCATAGCAGCAAAACCACCCCGACCACCCTGACCGCAAATTTTGTTCAGAATATTCGTTTCGTGCGCAAAAATGTTGAACAGAGCGGTCGTAATACCAATCCGCTGCAGTTTGTTGAACTGGAAACCGAATTTCCGCGTGATTATGATGATAACATTGAAATGCTGAGCCGTGAAGCAGAACATCTGGAAGAACAGTTTCGTACCCCGACCCGTAGCAATGCAACCGATGCAACCAGCCATCATGTTGCAGGTATTATTGATAACATCATTACCGAAGCAAGCCGTAGCCTGACCATTGAAAAAACCGAAGATGATGTTCCGCTGAAAAGCAGTACCAAACATAGCAGCGGTGTTAAACGTGTTGGTTTTCAGGTTGAGGATAAAGATGATACCGAAGTGCAGAGCGAAAAACAGGCAAAAAGCTTTGAGGATATTACCAAAAAAGCCGAAAGCAGCGAAGCCAGCGCAGAAGAAGCAGCAGTTACCGGTAGCAGCACCGATGCGAGCGCAAGTCCGCTGCCGAGCACCAGCCTGGTTAGCACCACCAGTAGCGCAACCAGCATTACCAAATCAAAATCCGATGAGGATGATGATCCGGTTGCAATGAGCCCGTGTGGTCGCTTTTTCAAATATGATAAAGAAGTTGGTCGCGGTAGCTTCAAAACCGTTTATCGTGGTCTGGATACCCTGACAGGTGTTCCGGTTGCCTGGTGTGAACTGCTGGATAAACAGGTGAAAAAAAGCGAACGTACCCGTTTTCGTGAAGAGGCAGATATGCTGAAAAAACTGCAACATCCGAATATCGTGCGCTTCTATACCTATTGGGAATTTCCGATTGGTCGCAAAAAAAACATTGTTCTGGTTACCGAACTGATGCTGAGCGGCACCCTGAAAAGCTATCTGAAACGCTTCAAAAAAATCCACCCGAAAGTTCTGAAAAGTTGGTGTCGTCAGATTCTGAAAGGTCTGAATTTTCTGCATACCCGTCAGTTTCCGATTATTCATCGTGATCTGAAATGCGACAACATTTTCATTACCGGTACAACCGGTAGCGTGAAAATTGGTGATCTGGGTCTGGCAACACTGAAAAATCGTAGCCATGCAAAAAGCGTTATTGGCACACCGGAATTTATGGCACCGGAAATGTATGAAGAACACTATGATGAAAGCGTGGATGTGTATGCATTTGGTATGTGCATGCTGGAAATGGCGATTAGCGAATATCCGTATAGCGAATGTAAAGGTCCGGCACAGATTTACAAAAAAGTGATTAGCGGTATTAAACCGGCAGCACTGGCAAAAGTTGAAGATCCGAATGTTCGCGATATTATCGAACGTTGCATCGAACTGAAAAAAGAAGATCGTCCGAGCTGTAATGAGCTGCTGGAAAGTGAATTTTTTGATGAGGATATTGGCATTCGTGTTGAACCGACCGCAAGCGAATAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEARKSSSDTVIEPLIKQQ
APLHSSKTTPTTLTANFVQNIRFVRKNVEQSGRNTNPLQFVELETEFPRD
YDDNIEMLSREAEHLEEQFRTPTRSNATDATSHHVAGIIDNIITEASRSL
TIEKTEDDVPLKSSTKHSSGVKRVGFQVEDKDDTEVQSEKQAKSFEDITK
KAESSEASAEEAAVTGSSTDASASPLPSTSLVSTTSSATSITKSKSDEDD
DPVAMSPCGRFFKYDKEVGRGSFKTVYRGLDTLTGVPVAWCELLDKQVKK
SERTRFREEADMLKKLQHPNIVRFYTYWEFPIGRKKNIVLVTELMLSGTL
KSYLKRFKKIHPKVLKSWCRQILKGLNFLHTRQFPIIHRDLKCDNIFITG
TTGSVKIGDLGLATLKNRSHAKSVIGTPEFMAPEMYEEHYDESVDVYAFG
MCMLEMAISEYPYSECKGPAQIYKKVISGIKPAALAKVEDPNVRDIIERC
IELKKEDRPSCNELLESEFFDEDIGIRVEPTASE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU48518
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 214-716. Single silent change as compared to the reference; T>G 495 bp.