cDNA Clone ABIN1

• Synonyms: TNIP1, ABIN-1, NAF1, VAN, nip40-1
• Plasmid: pMK-RQ ABIN1 GFP Donor
• Parent Plasmid: pMK-RQ
• Genbank: NM_001252385.1
• Species: Human

Sequence of Insert

TGACAGTGTATTAAATATTAGTCAGGGTGTTTTTTTCTTTTCTTTTTTTAAAGAAGATGATACACATATAAATGGAACTTTGGACTGGGTTGACTTGCTTATCCCTTAGGGTACAAGCATTTCACTTTAAAGATTACTAAATTAACTTAAGACTTTCATTTCCCTCCCTCAGAGAGGGGAAGGGACTGCCCATAATCCCATAGTAAATTAATGCCTAGACCATTAATTGAGTAAGTGTTTATTGGGCAAAATTCATAGCTAATACTGTGGTAGGCTCTGAGGATGCAATGATGGGTAAAACATATGGGATTCCTGTTCTTCTGGACCTCACAGACAGGACATGAACTCAGATTTTTTTTTTTCCAAGTCCAGGTTCTTTCTTGTATCCCACATTGCACCCTACTGTTTATTTGGACTCCTGTTCCAAACAAGCCAACAGTGCATTGAGGCTGCTAAGAGAAACTAATGCCATTCTAAGGTTGTATTAATAGAGGCAGAGCGTCCAGATAAAAGGAGGTGACTGTCCTGCTTTACTATAATATGGTCAGACCAGCTTAAAGTGGGGGACTGCCTTCTGGGGCATTGACAGACTAGAGCTTCTCTGCAAAGGATGCCCCCATGGCCTGGCCAGCCTGCTGATATGCTGCTGTTGTCCTTACAGCCAGGCGGGCACCACGGCAGGGGCTGAGCTACCCTCatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagagcggttctGAgGGacGtGGACCGTACaGaATaTAtGAtCCTGGGGGCAGCGTGCCCTCAGGAGAGGCATCCGCAGCTTTTGAGCGCCTAGTGAAGGAGAATTCCCGGCTGAAGGAAAAAATGCAAGGGATAAAGATGTTAGGTAAAGCAGACCCTGTCCCCTCCCTGCGAGCGCCCCTCCCTGCAGACTGTGATGCCAGTCCCTCTACTAGCAGTGGAGAATGAAGCTGGCATATGCCTCTGCCCAAGTCATCCCCTGTCCCTCCTGGCCCAGCGCTGGCTCCTGCCTCACAGCTGTCTCTATGCACCATGTAGCATGGTGACAGTATTCCCAACCCTGATTCCTCAGCACCCGGAAACCAGGATGACAGGACTCAGGTCCAAGAGGGAGGCGTGGGCTAAGGGAAAGGGGGCTGGAAGGCCCAGTTAATGGCCATGGAACTAGAGTGGTCTCCTCTTTCTGGGGCACGAGAGATGGGGAGAGCCAGCATGACCAGGCAACTGGGGCACGCGTTCCTAGGTGTTGGAGCAGGGAGCAGAGCTTGGCTTGAAGCAAGGAGGTGGTAGGTCCCTCAGTTCTCTTCTCCTGGCTCTTGCTCAAGCCATCCCCACTTTAGAACAACCCCACCTCCCTCCACACGACCGACGGGCCCTGTGCATCAGCACCCAGTTGCTGTGCCCCCTTCTCCTCCAGACAAGGCTTTCATTGATTTCCCCATCCAGAGGGGGCTCTCTTTCT

• Vector Type: Other
• Antibiotic: Kan
• DU Number: DU48524
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Designed to insert an in-frame GFP into the ATG start of the first common coding exon (exon 2 isoform 1). Complete sequence of donor including homology arms shown as sequence of insert, contains several silent mutations to prevent Cas9 targeting the donor sequence. Successful recombination will N-terminally tag Abin1 with GFP.