cDNA Clone
DNA2 (yeast)

Plasmid
pGADT7 5GA DNA2 (yeast) D121A D213A L215A I218A
Parent Plasmid
pGADT7
Expressed
GAL4 AD-HA5GA DNA2 (yeast) D121A D213A L215A I218A
Genbank
NP_012034.1
Tag
HA
GAL4 DNA-AD
Species
Yeast
Sequence of Insert
GAATTCGGAGCTGGTGCAGGCGCTGGAGCGGGTGCCATGCCCGGAACGCCACAGAAGAACAAGAGGTCTGCGAGTATATCTGTTTCACCTGCGAAGAAGACAGAGGAAAAAGAAATAATACAAAATGATTCAAAGGCGATATTATCTAAGCAAACTAAGAGGAAAAAAAAGTATGCTTTTGCTCCTATAAATAACTTAAATGGGAAGAACACCAAGGTATCCAATGCAAGTGTCCTAAAGTCAATAGCCGTTTCACAAGTACGAAATACATCAAGAACAAAAGATATAAACAAAGCAGTTAGCAAGAGCGTAAAGCAATTACCAAATTCACAAGTTAAACCGAAACGGGAAATGTCTAATTTGAGTAGGCATCACGATTTCACTCAAGACGAGGACGGCCCCATGGAAGAAGTAATATGGAAATATTCACCTTTACAACGAGATATGTCCGACAAAACAACAAGCGCTGCCGAATACTCTGATGACTACGAAGATGTCCAAAATCCCTCTTCTACACCTATAGTCCCTAATCGACTGAAAACTGTTCTTAGTTTCACAAATATCCAAGTACCCAACGCTGACGTAAATCAACTCATTCAAGAAAATGGAAATGAGCAAGTGCGTCCTAAACCAGCAGAAATATCTACCAGGGAGTCTCTCCGTAATATAGCTGCTATTGCCGATGATGCAGAGGGCGATTTAACTATAAAACCGACGATAACGAAATTCAGCGATTTGCCATCTTCACCCATCAAGGCACCCAACGTTGAAAAAAAAGCAGAGGTGAATGCAGAAGAAGTAGATAAAATGGATTCAACAGGAGATAGCAACGATGGCGATGACTCTTTGATAGATATTTTAACTCAAAAGTATGTTGAGAAACGCAAGAGTGAGAGTCAGATAACAATTCAAGGCAACACCAATCAAAAAAGTGGAGCCCAGGAAAGTTGTGGGAAGAATGATAACACAAAATCGAGAGGAGAAATTGAAGATCATGAAAATGTAGACAATCAAGCCAAAACAGGCAATGCATTTTATGAGAATGAAGAAGACAGTAATTGCCAAAGAATTAAAAAAAATGAAAAAATAGAATACAATTCTTCAGACGAATTTTCAGACGATTCACTAATTGAACTATTGAACGAAACTCAAACACAAGTGGAACCTAACACTATTGAACAAGATTTAGACAAAGTGGAGAAAATGGTCTCTGACGATTTGAGAATTGCTACTGATTCCACTTTATCCGCATATGCTCTCAGAGCTAAGTCTGGTGCTCCAAGAGACGGCGTAGTACGATTAGTAATAGTAAGCTTACGTAGTGTTGAATTGCCAAAAATTGGAACGCAAAAAATACTAGAGTGTATAGACGGCAAAGGCGAACAAAGTTCTGTTGTCGTTCGACATCCTTGGGTATATTTGGAATTTGAGGTTGGGGATGTAATACATATTATAGAAGGGAAAAATATTGAAAACAAACGGCTTCTTTCTGACGACAAAAACCCTAAAACTCAACTGGCTAACGACAACCTGTTGGTGCTAAACCCTGATGTATTATTTTCGGCAACATCAGTCGGAAGTTCAGTAGGTTGTTTAAGACGTTCAATTCTGCAAATGCAATTTCAAGATCCCCGCGGAGAACCAAGCCTTGTCATGACTTTAGGCAATATCGTACACGAGTTATTGCAAGACTCAATCAAATACAAGCTCTCGCATAATAAAATTTCTATGGAAATCATAATACAAAAGCTGGATTCTTTGTTAGAAACTTATTCTTTTTCAATTATCATTTGTAATGAAGAAATCCAGTACGTTAAAGAATTAGTTATGAAAGAACATGCTGAAAACATTTTGTACTTCGTCAATAAATTTGTTTCGAAAAGTAACTATGGTTGCTACACATCAATTTCCGGAACAAGAAGAACACAGCCAATTTCTATATCCAATGTGATTGATATTGAAGAAAACATTTGGTCTCCAATATATGGTCTTAAAGGGTTCCTAGATGCAACTGTTGAAGCTAATGTTGAAAACAATAAAAAACACATCGTTCCTTTAGAAGTGAAAACTGGAAAATCCAGAAGCGTTTCATACGAGGTTCAAGGCCTTATTTATACACTTCTGTTAAATGATCGCTATGAGATACCGATAGAGTTTTTTCTTCTTTATTTTACGAGGGATAAAAATATGACAAAGTTCCCTTCTGTTCTGCACTCCATTAAACATATTCTCATGTCCCGAAATAGAATGAGTATGAATTTTAAGCATCAACTCCAAGAGGTTTTCGGACAGGCTCAATCAAGGTTTGAATTACCACCCTTACTGCGCGATTCATCTTGTGATTCATGTTTCATCAAAGAATCATGCATGGTGTTGAATAAGCTACTAGAGGACGGGACACCAGAAGAAAGTGGCCTGGTAGAAGGTGAGTTTGAAATTTTGACAAACCATTTGTCGCAAAATCTCGCCAACTACAAAGAGTTTTTTACAAAATATAATGATCTAATAACTAAGGAGGAGTCAAGTATAACATGTGTAAATAAAGAACTTTTCTTATTGGATGGTTCCACAAGAGAATCCAGAAGTGGAAGGTGCCTTTCTGGCTTGGTTGTGTCAGAGGTGGTTGAACATGAAAAAACAGAGGGAGCGTATATATATTGCTTCTCAAGACGTAGAAATGATAATAACTCGCAGTCGATGTTATCATCTCAAATTGCTGCTAATGACTTTGTTATTATTAGTGATGAGGAGGGTCATTTTTGCTTGTGCCAAGGTCGTGTACAATTTATAAATCCCGCTAAAATTGGTATCTCCGTAAAACGAAAGTTATTAAACAATAGGCTTCTAGATAAGGAAAAAGGAGTCACTACCATCCAAAGTGTGGTCGAATCAGAGCTAGAACAATCCAGTCTCATAGCTACCCAAAATTTGGTCACATATAGAATCGACAAAAATGATATCCAACAGAGTTTGTCTTTAGCGCGATTTAATCTTTTAAGCTTGTTTTTACCAGCTGTTTCACCTGGCGTGGATATTGTTGACGAAAGGAGTAAATTATGTCGGAAGACAAAGAGATCTGATGGAGGTAATGAAATACTGCGATCACTTTTAGTTGATAATAGAGCACCTAAGTTTAGAGATGCAAATGATGATCCCGTCATTCCGTATAAGCTTTCAAAGGATACAACATTGAACTTGAATCAAAAAGAAGCAATAGACAAAGTAATGAGGGCCGAAGATTATGCATTGATATTGGGAATGCCAGGGACTGGTAAGACTACTGTTATCGCAGAAATTATTAAAATTCTCGTTTCCGAGGGGAAACGCGTGCTTTTGACATCATACACACATTCAGCAGTGGATAATATTTTAATAAAATTGAGGAACACCAACATTAGCATAATGAGATTGGGAATGAAACATAAGGTCCATCCTGACACGCAAAAATATGTCCCGAATTACGCGTCAGTGAAAAGTTATAACGATTATTTGTCCAAAATTAACAGCACCTCTGTTGTTGCTACCACCTGCTTAGGAATTAACGATATACTATTTACACTAAATGAAAAGGATTTTGATTATGTGATTTTGGACGAGGCAAGTCAAATTTCAATGCCTGTCGCTTTGGGACCTCTGCGGTATGGAAATAGGTTTATTATGGTAGGAGATCATTACCAGCTACCGCCATTAGTTAAAAATGACGCAGCTCGATTAGGTGGGTTAGAAGAGTCACTGTTCAAGACTTTCTGTGAAAAGCATCCGGAAAGCGTTGCAGAACTGACTTTACAGTACCGTATGTGCGGCGATATTGTAACTTTGTCTAATTTCCTAATTTATGATAATAAATTAAAATGCGGGAATAACGAAGTTTTTGCTCAATCTTTAGAGTTACCAATGCCTGAGGCCTTGTCGCGCTATAGGAATGAATCGGCTAACTCTAAGCAGTGGCTTGAGGATATACTAGAGCCTACAAGAAAAGTGGTATTTTTAAATTACGATAACTGCCCGGATATTATCGAACAAAGCGAAAAGGATAACATTACGAATCACGGGGAGGCAGAATTGACTCTACAATGTGTGGAAGGAATGTTATTGAGCGGCGTACCATGCGAAGATATTGGTGTAATGACGTTATACAGAGCACAGTTAAGATTACTGAAGAAGATATTCAACAAAAATGTGTATGACGGGCTAGAGATCTTGACTGCTGATCAGTTTCAAGGTCGTGACAAAAAGTGCATTATTATTTCCATGGTTAGAAGAAATTCTCAATTAAATGGAGGAGCGCTGCTGAAAGAGCTAAGAAGAGTCAATGTTGCCATGACAAGGGCCAAATCCAAGCTGATCATTATAGGTTCAAAAAGTACAATCGGCTCTGTTCCTGAAATAAAATCGTTCGTGAACCTGTTAGAAGAGCGTAATTGGGTCTATACAATGTGCAAGGATGCACTCTACAAATACAAATTTCCCGACAGATCAAACGCTATTGATGAGGCACGCAAAGGTTGTGGAAAAAGAACAGGTGCCAAACCCATCACATCAAAGTCTAAATTCGTAAGCGATAAACCTATCATAAAGGAAATTCTACAAGAGTATGAAAGTTGAGGATCC
Amino Acid Sequence
MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIFNTTHYRASAAMEYPYDVPDYAHMAMEASEFGAGAGAGAGAMPGTPQKNKRSASISVSPAKKTEEKEIIQNDSKAILSKQTKRKKKYAFAPINNLNGKNTKVSNASVLKSIAVSQVRNTSRTKDINKAVSKSVKQLPNSQVKPKREMSNLSRHHDFTQDEDGPMEEVIWKYSPLQRDMSDKTTSAAEYSDDYEDVQNPSSTPIVPNRLKTVLSFTNIQVPNADVNQLIQENGNEQVRPKPAEISTRESLRNIAAIADDAEGDLTIKPTITKFSDLPSSPIKAPNVEKKAEVNAEEVDKMDSTGDSNDGDDSLIDILTQKYVEKRKSESQITIQGNTNQKSGAQESCGKNDNTKSRGEIEDHENVDNQAKTGNAFYENEEDSNCQRIKKNEKIEYNSSDEFSDDSLIELLNETQTQVEPNTIEQDLDKVEKMVSDDLRIATDSTLSAYALRAKSGAPRDGVVRLVIVSLRSVELPKIGTQKILECIDGKGEQSSVVVRHPWVYLEFEVGDVIHIIEGKNIENKRLLSDDKNPKTQLANDNLLVLNPDVLFSATSVGSSVGCLRRSILQMQFQDPRGEPSLVMTLGNIVHELLQDSIKYKLSHNKISMEIIIQKLDSLLETYSFSIIICNEEIQYVKELVMKEHAENILYFVNKFVSKSNYGCYTSISGTRRTQPISISNVIDIEENIWSPIYGLKGFLDATVEANVENNKKHIVPLEVKTGKSRSVSYEVQGLIYTLLLNDRYEIPIEFFLLYFTRDKNMTKFPSVLHSIKHILMSRNRMSMNFKHQLQEVFGQAQSRFELPPLLRDSSCDSCFIKESCMVLNKLLEDGTPEESGLVEGEFEILTNHLSQNLANYKEFFTKYNDLITKEESSITCVNKELFLLDGSTRESRSGRCLSGLVVSEVVEHEKTEGAYIYCFSRRRNDNNSQSMLSSQIAANDFVIISDEEGHFCLCQGRVQFINPAKIGISVKRKLLNNRLLDKEKGVTTIQSVVESELEQSSLIATQNLVTYRIDKNDIQQSLSLARFNLLSLFLPAVSPGVDIVDERSKLCRKTKRSDGGNEILRSLLVDNRAPKFRDANDDPVIPYKLSKDTTLNLNQKEAIDKVMRAEDYALILGMPGTGKTTVIAEIIKILVSEGKRVLLTSYTHSAVDNILIKLRNTNISIMRLGMKHKVHPDTQKYVPNYASVKSYNDYLSKINSTSVVATTCLGINDILFTLNEKDFDYVILDEASQISMPVALGPLRYGNRFIMVGDHYQLPPLVKNDAARLGGLEESLFKTFCEKHPESVAELTLQYRMCGDIVTLSNFLIYDNKLKCGNNEVFAQSLELPMPEALSRYRNESANSKQWLEDILEPTRKVVFLNYDNCPDIIEQSEKDNITNHGEAELTLQCVEGMLLSGVPCEDIGVMTLYRAQLRLLKKIFNKNVYDGLEILTADQFQGRDKKCIIISMVRRNSQLNGGALLKELRRVNVAMTRAKSKLIIIGSKSTIGSVPEIKSFVNLLEERNWVYTMCKDALYKYKFPDRSNAIDEARKGCGKRTGAKPITSKSKFVSDKPIIKEILQEYES*
Vector Type
Yeast
Antibiotic
Amp
DU Number
DU26023
Unit Source
Protein Phosphorylation and Ubiquitylation Unit
Location
PPU
MTA
Dundee
Price
£125.00
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