cDNA Clone ZGRF1

• Synonyms: C4orf21
• Plasmid: pcDNA5D FRT/TO GFP ZGRF1-HA
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP-ZGRF1-HA
• Genbank: NM_018392.4 GI:254826808 with silent change g5505a
• Tag: HA
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGAAAGCCAAGAATTTATTGTTCTATATACTCATCAAAAGATGAAGAAGTCAAAAGTGTGGCAAGATGGAATTCTGAAGATCACTCACTTAGGAAACAAAGCAATTTTATATGATGACAAAGGAGCATGTTTGGAGAGTCTGTTTCTTAAATGCCTTGAGGTGAAACCTGGAGATGACTTAGAAAGTGATCGATACTTAATCACAGTTGAAGAGGTTAAAGTTGCTGGAGCCATAGGTATTGTTAAGCAGAATGTCAATAAAGAAGCACCAGAGTTAAATTCAAGGACATTTATATCCTCTGGCCGATCTCTTGGATGTCAGCCCTCTGGCTTAAAAAGGAAGTTTACTGGTTTTCAAGGACCACGTCAGGTTCCAAAGAAAATGGTTATTATGGAAAGTGGTGAATCAGCTGCATCACATGAGGCTAAGAAAACTGGCCCTACTATTTTTTCTCCATTCTGCAGCATGCCTCCTTTGTTTCCTACTGTTGGCAAGAAAGATGTAAATAATATACTGGCAGACCCTGAGAACATTGTGACTTACAAGAACAGGGAGAGAAATGCCATGGATTTTTCTTCGGTTTTTTCTCCATCCTTCCAGATTAACCCAGAAGTGCTGTGTGAAGAAAATTATTTTTGCTCACCTGTCAATTCTGGAAATAAGCTTTCAGACTCTTTACTGACCAATGAGCCTGTGAAAAGAGATAGTTTGGCATCTCACTATTCAGGAGTTTCACAAAACATCAGAAGCAAAGCACAGATATTAGCTCTTCTGAAGTCCGAATCATCTAGTTCATGTGAGGAACTAAATTCTGAGATGACAGAGCATTTTCCTCAAAAACAACCACAAGGAAGTTTAAAAATTGCTACTAAACCAAAGTACCTAATTCAACAGGAAGAGTGTGCTGAGATGAAGAGCACAGAAAATTTATACTACCAGCATCAATCAGAAAATACCATGAGAAATAAAAGCCGGTGGGCCATGTATTTATCCTCACAGAGTTCACCTATACATTCTTCTACTGTAGATGGGAATGATACAGAAAGGAAACCCAAGGCCCAGGAAGATGATGTAAATTCTAATTTGAAAGACCTTTCATTACAAAAAATTATACAGTTCGTTGAAACGTATGCTGAAGAGAGGAAAAAGTATAATGTAGACCAGTCAGTCGGTAATAATGATCCATCCTGGAATCAGGAAGTAAAATTAGAAATTCCTTCATTCAATGAAAGCAGTAGCTTACAGGTTACTTGTAGCAGTGCAGAAAATGATGGTATATTATCAGAATCTGACATTCAAGAAGATAATAAAATACCTTTTAATCAAAATGACAAGGGGTGCATTAAAGGATCAGTTCTCATTAAAGAAAATGCTCAGGAGGTAAATACATGTGGAACACTGGAAAAGGAGTATGAACAATCAGAGTCATCTCTGCCAGAACTGAAACATCTCCAAATTGAATCTAGTAATAATTCTAGGATCTCTGATGACATTACAGACATGATTTCTGAAAGTAAAATGGATAATGAAAGTCTTAATAGTATTCATGAATCTCTGAGTAATGTAACACAACCATTTTTGGAGGTAACTTTTAATCTGAACAATTTTGAGACCAGTGACACTGAGGAGGAATCACAGGAAAGCAACAAAATTTCCCAGGATTCAGAGAGTTGGGTAAAGGACATTTTGGTTAATGATGGCAATTCATGTTTTCAAAAGAGGTCTGAGAATACAAACTGTGAAGAGATTGAGGGTGAACATTTGCCATTCTTAACATCTGTTAGTGACAAACCTACAGTGACATTTCCTGTTAAAGAGACTCTGCCATCACAGTTTTGTGATAAAACTTATGTGGGTTTTGACATGGGAATATGTAAAACTGAAAACACAGGAAAAGAAATAGAGGAGTATAGTGACACATTAAGCAATTTTGAATCTTTCAAGTGGACTGATGCTGTATACGGAGATAATAAAGAAGATGCTAATAAACCTATTCAAGAAGTCAGAATTAACTATGATTTTGCTTTACCCCCGAATAAATCTAAAGGTATAAACATGAATTTACATATTCCTCATATTCAAAACCAGATTGCTGAAAACAGTAATCTATTTTCAGAAGATGCTCAACCTCAGCCTTTTATTTTGGGAAGTGACTTAGACAAAAATGATGAACATGTTTTACCCTCAACTTCTAGTAGTGACAACAGTGTCCAACTATTAAATACCAATCAGAATCACTATGAATGTATTGCACTTGATAAATCAAATACCCACATTTCCAATTCTTTGTTTTACCCACTGGGAAAAAAGCATCTTATTTCCAAAGACACAGAAGCACATATATCTGAACCTGAAGATTTGGGAAAGATTAGAAGTCCACCCCCTGACCATGTTGAGGTTGAAACTGCCAGAGAAGGCAAACAATACTGGAATCCTAGAAATTCTTCAGAACTTTCTGGATTAGTAAATACCATTTCTATTTTAAAGTCGCTATGTGAACACAGTACTGCTTTAGACAGCTTGGAAATATTGAAAAAGAAAAATACTGTCTTTCAGCAAGGAACTCAACAGACGTATGAGCCAGATAGCCCTCCTGAAGTGAGGAAACCATTTATTACAGTAGTTTCACCAAAGTCTCCTCATCTGCACAAGGATTCTCAACAGATACTAAAAGAAGATGAAGTTGAACTAAGTGAACCACTTCAGTCTGTGCAGTTCTCTTCCTCGGGAAGTAAAGAAGAGACTGCTTTTCAAGCTGTTATTCCTAAACAAATAGAGAGAAAAACCTGTGACCCTAAGCCTGTTGAGTTTCAAGGACATCAAGTAAAAGGATCAGCTACTAGTGGTGTAATGGTCAGAGGACACAGCTCACAGCTAGGATGCAGTCAGTTTCCAGATAGCACTGAGTATGAAAACTTTATGACAGAGACACCAGAGTTACCTAGCACGTGTATGCAGATTGACTTCTTGCAGGTGACATCACCAGAAGAAAACATCTCTACATTGAGCCCTGTTTCTACCTTTTCTTTGAACTCAAGAGATGAAGACTTCATGGTAGAATTCTCTGAGACGTCCCTGAAAGCAAGAACTTTACCTGATGATCTTCATTTTCTCAACTTGGAGGGGATGAAAAAATCCCGTTCTCTGGAGAATGAGAACCTTCAAAGGCTTTCATTATTAAGTAGAACCCAGGTTCCACTTATTACTTTGCCACGTACTGATGGGCCACCTGACTTAGACTCTCATTCGTATATGATCAACTCTAACACATACGAGTCTTCTGGCTCCCCCATGCTCAATTTGTGTGAAAAGTCAGCAGTTCTTTCGTTTAGCATTGAGCCTGAGGACCAAAATGAAACCTTTTTCTCTGAAGAATCTAGGGAAGTGAATCCAGGGGATGTTTCACTTAATAATATATCTACTCAGAGCAAGTGGCTGAAATATCAAAACACATCCCAATGCAACGTGGCTACTCCAAACAGAGTTGATAAGAGAATAACTGATGGCTTCTTTGCTGAGGCTGTTTCTGGGATGCATTTTAGAGACACAAGTGAAAGACAGAGTGATGCTGTCAATGAAAGCTCTTTAGACTCTGTGCATTTGCAAATGATAAAAGGCATGCTCTATCAACAGCGGCAGGATTTTAGCAGTCAAGATTCGGTTTCCAGAAAGAAAGTACTTTCTCTGAATTTAAAGCAGACTTCTAAGACAGAGGAAATTAAAAATGTATTAGGAGGGTCTACCTGCTACAACTACAGTGTAAAGGATTTACAGGAGATAAGTGGCTCTGAGCTGTGCTTTCCAAGTGGGCAGAAAATAAAATCTGCTTATCTTCCCCAAAGGCAAATTCACATACCAGCTGTTTTTCAGTCTCCTGCTCATTATAAGCAGACTTTCACATCTTGCCTCATAGAACATCTAAATATATTGCTGTTTGGGTTAGCACAAAACCTGCAGAAAGCTCTTTCAAAAGTTGACATATCATTTTATACATCATTGAAGGGAGAGAAACTGAAAAACGCAGAAAATAATGTACCATCCTGCCATCATAGTCAACCTGCAAAACTTGTCATGGTTAAAAAGGAAGGTCCAAATAAGGGTCGTCTCTTTTATACATGTGATGGACCCAAAGCTGATCGATGTAAATTCTTTAAATGGCTTGAGGACGTGACTCCAGGATATTCAACACAGGAAGGAGCTCGACCTGGCATGGTTTTAAGTGATATTAAGAGTATTGGCTTATATTTAAGAAGTCAAAAGATACCACTTTATGAGGAATGCCAGCTTTTGGTGAGAAAAGGATTTGATTTTCAGAGAAAACAGTATGGCAAACTAAAGAAGTTTACTACTGTAAATCCTGAGTTTTATAATGAACCAAAAACCAAACTTTATCTTAAGCTAAGTCGGAAGGAAAGATCTTCAGCTTATAGCAAAAATGATCTTTGGGTGGTTTCAAAAACCCTAGACTTTGAGCTGGATACTTTTATCGCATGTAGTGCTTTCTTTGGACCATCATCTATCAATGAGATAGAAATACTGCCTTTGAAAGGCTATTTCCCTTCTAATTGGCCCACTAACATGGTTGTCCATGCGTTATTGGTTTGTAATGCTAGCACAGAACTGACTACTTTGAAAAACATTCAGGACTACTTTAATCCAGCTACTCTACCTCTAACACAGTACCTGTTAACAACGTCTTCGCCAACTATAGTTAGTAACAAAAGAGTCAGTAAGAGAAAATTTATCCCACCAGCCTTCACAAATGTCAGTACAAAATTTGAACTACTCAGCCTAGGAGCAACATTGAAGTTAGCTAGTGAGTTGATTCAGGTACACAAGTTAAACAAGGATCAAGCTACAGCTCTAATTCAAATAGCTCAAATGATGGCATCACATGAAAGCATTGAAGAAGTGAAGGAACTGCAAACTCATACCTTCCCTATCACAATCATACATGGTGTGTTTGGAGCAGGAAAGAGTTACTTGCTGGCAGTGGTGATTTTGTTCTTTGTACAGCTGTTTGAAAAGAGTGAAGCTCCCACCATTGGAAATGCAAGGCCGTGGAAACTTCTGATTTCTTCTTCTACTAATGTGGCTGTTGACAGAGTACTTCTTGGGCTTCTCAGTCTTGGATTTGAAAACTTTATCAGAGTTGGGAGTGTTAGGAAGATTGCCAAACCAATTTTACCTTATAGCTTGCATGCTGGCTCAGAAAATGAAAGTGAACAGTTAAAAGAACTACATGCACTAATGAAAGAAGACCTGACTCCTACGGAAAGAGTCTATGTGAGAAAAAGCATTGAGCAGCATAAACTGGGGACCAATAGAACCCTGCTGAAGCAGGTTCGAGTAGTTGGAGTTACCTGTGCAGCCTGCCCATTCCCATGCATGAATGATCTTAAATTTCCTGTAGTTGTGCTGGATGAGTGTAGTCAGATAACTGAACCGGCCTCTCTCCTTCCCATTGCAAGGTTTGAGTGTGAAAAGCTGATTCTTGTTGGAGATCCCAAACAGCTACCTCCTACTATTCAGGGTTCTGATGCAGCTCATGAAAATGGATTGGAACAAACTCTTTTTGATCGACTTTGCTTAATGGGTCACAAGCCAATTCTATTGAGAACTCAATACCGTTGTCATCCTGCAATCAGTGCTATTGCTAATGATCTGTTTTACAAAGGAGCCCTCATGAATGGTGTAACAGAAATAGAGCGGAGCCCTTTATTGGAATGGCTACCAACCCTGTGTTTTTATAATGTTAAAGGACTAGAACAGATAGAAAGAGATAACAGCTTTCATAATGTGGCAGAAGCTACGTTTACACTCAAGCTGATTCAATCACTGATTGCAAGTGGAATAGCAGGCTCTATGATTGGTGTGATAACATTATACAAATCCCAGATGTACAAGCTTTGTCATTTACTCAGTGCTGTGGACTTTCACCATCCTGATATTAAAACTGTGCAGGTGTCCACAGTAGATGCTTTTCAGGGAGCTGAAAAGGAGATCATTATTCTGTCCTGTGTAAGGACAAGACAAGTAGGATTCATTGATTCAGAAAAAAGAATGAATGTTGCATTGACTAGAGGAAAGAGGCATTTGTTGATTGTGGGAAATTTAGCCTGTTTGAGGAAAAATCAACTTTGGGGACGAGTGATCCAACACTGCGAAGGAAGGGAAGATGGATTGCAACATGCAAACCAGTATGAACCACAGCTGAACCATCTCCTTAAAGATTATTTTGAAAAACAAGTGGAAGAAAAACAGAAGAAAAAGAGTGAAAAAGAGAAATCTAAAGATAAATCTCATTCAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMESQEF
IVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGACLESLFLKCLEVK
PGDDLESDRYLITVEEVKVAGAIGIVKQNVNKEAPELNSRTFISSGRSLG
CQPSGLKRKFTGFQGPRQVPKKMVIMESGESAASHEAKKTGPTIFSPFCS
MPPLFPTVGKKDVNNILADPENIVTYKNRERNAMDFSSVFSPSFQINPEV
LCEENYFCSPVNSGNKLSDSLLTNEPVKRDSLASHYSGVSQNIRSKAQIL
ALLKSESSSSCEELNSEMTEHFPQKQPQGSLKIATKPKYLIQQEECAEMK
STENLYYQHQSENTMRNKSRWAMYLSSQSSPIHSSTVDGNDTERKPKAQE
DDVNSNLKDLSLQKIIQFVETYAEERKKYNVDQSVGNNDPSWNQEVKLEI
PSFNESSSLQVTCSSAENDGILSESDIQEDNKIPFNQNDKGCIKGSVLIK
ENAQEVNTCGTLEKEYEQSESSLPELKHLQIESSNNSRISDDITDMISES
KMDNESLNSIHESLSNVTQPFLEVTFNLNNFETSDTEEESQESNKISQDS
ESWVKDILVNDGNSCFQKRSENTNCEEIEGEHLPFLTSVSDKPTVTFPVK
ETLPSQFCDKTYVGFDMGICKTENTGKEIEEYSDTLSNFESFKWTDAVYG
DNKEDANKPIQEVRINYDFALPPNKSKGINMNLHIPHIQNQIAENSNLFS
EDAQPQPFILGSDLDKNDEHVLPSTSSSDNSVQLLNTNQNHYECIALDKS
NTHISNSLFYPLGKKHLISKDTEAHISEPEDLGKIRSPPPDHVEVETARE
GKQYWNPRNSSELSGLVNTISILKSLCEHSTALDSLEILKKKNTVFQQGT
QQTYEPDSPPEVRKPFITVVSPKSPHLHKDSQQILKEDEVELSEPLQSVQ
FSSSGSKEETAFQAVIPKQIERKTCDPKPVEFQGHQVKGSATSGVMVRGH
SSQLGCSQFPDSTEYENFMTETPELPSTCMQIDFLQVTSPEENISTLSPV
STFSLNSRDEDFMVEFSETSLKARTLPDDLHFLNLEGMKKSRSLENENLQ
RLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKS
AVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCN
VATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIK
GMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVK
DLQEISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIE
HLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAK
LVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMV
LSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEF
YNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPS
SINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPAT
LPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLA
SELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVF
GAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLL
GLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDL
TPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVV
VLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLE
QTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERS
PLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGS
MIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIIL
SCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHC
EGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHSAA
AYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49946
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Recommend propagation at 30 degrees.