cDNA Clone ALMS1

• Synonyms: Alstrom syndrome 1 , ALSS
• Plasmid: pSc-B-ALMS1 nt1-3278
• Parent Plasmid: pSC-B
• Genbank: NM_015120.4 NP_055935.4
• Species: Human

Sequence of Insert

gtcgacATGGAGCCCGAGGATCTGCCATGGCCGGGCGAGCTGGAGGAGGA
GGAGGAGGAGGAGGAGGAGGAGGAAGAGGAGGAGGCTGCAGCGGCGGCGG
CGGCGAACGTGGACGACGTAGTGGTCGTGGAGGAGGTGGAGGAAGAGGCG
GGGCGGGAGTTGGACTCCGACTCTCACTACGGGCCCCAGCATCTGGAAAG
TATAGACGACGAGGAGGACGAGGAGGCCAAGGCCTGGCTGCAGGCGCACC
CCGGCAGGATTTTGCCTCCGCTGTCGCCCCCGCAGCACCGCTACTCGGAG
GGCGAGCGGACCTCCCTGGAGAAGAAAACAGAATCTTGGCATTGTCTTCC
TCAAGAAATGGACTCTTCCCAAACCTTGGATACATCCCAGACTAGGTTTA
ATGTGAGAACGGAAGATACTGAAGTGACAGACTTCCCCTCTCTGGAGGAG
GGCATATTGACGCAATCAGAAAATCAAGTAAAGGAACCCAACAGAGATCT
CTTCTGTTCTCCACTGCTAGTCATACAAGATAGCTTTGCTTCTCCTGATT
TGCCTTTGCTGACCTGTTTGACACAAGACCAAGAATTTGCGCCTGATTCT
TTATTTCATCAAAGTGAACTAAGTTTTGCACCTCTGAGGGGAATTCCTGA
TAAGTCTGAAGATACTGAATGGTCTTCTCGACCATCGGAAGTTAGTGAAG
CTTTATTCCAGGCTACTGCAGAAGTAGCTTCAGACTTAGCAAGCAGTCGC
TTTAGTGTATCTCAGCACCCGCTTATAGGCAGCACAGCTGTTGGGTCTCA
GTGCCCTTTTTTACCTTCTGAACAAGGGAATAATGAAGAGACTATTTCGT
CTGTTGATGAACTGAAAATTCCCAAAGACTGTGATCGTTATGATGATCTT
TGTTCATATATGTCATGGAAGACACGAAAAGATACACAGTGGCCTGAAAA
CAATTTAGCTGATAAAGATCAAGTTTCAGTTGCAACTTCATTTGACATAA
CTGATGAAAACATAGCTACTAAAAGAAGTGACCATTTTGATGCTGCTTGT
TCATATGGGCAGTATTGGACACAGGAAGATTCATCTAAGCAGGCAGAAAC
ATATTTAACCAAGGGCCTGCAGGGGAAGGTTGAGTCTGACGTCATTACTC
TGGATGGCCTAAATGAAAATGCTGTTGTATGCAGTGAAAGAGTTGCTGAA
CTACAAAGAAAGCCAACAAGAGAGTCGGAATATCACTCTTCAGATCTCAG
AATGTTGAGGATGTCTCCTGACACTGTGCCAAAGGCTCCTAAACATTTAA
AAGCAGGAGACACTTCTAAAGGAGGCATAGCTAAAGTTACTCAATCCAAC
TTGAAGTCAGGCATCACTACCACTCCTGTTGATTCAGACATTGGATCTCA
TTTATCCTTGTCCCTTGAGGACCTGTCTCAGTTGGCTGTAAGTTCTCTAG
AAACTACTACTGGTCAACACACTGATACTCTCAACCAAAAGACATTAGCA
GATACTCATCTAACTGAAGAGACTCTGAAAGTCACAGCTATTCCTGAACC
AGCTGACCAGAAGACTGCAACACCAACAGTACTCTCTAGTTCCCACTCAC
ATAGGGGGAAGCCCAGCATTTTCTACCAGCAGGGCTTGCCAGACAGTCAT
CTAACTGAAGAGGCTTTGAAAGTTTCAGCTGCTCCTGGACTAGCTGACCA
GACAACTGGCATGTCAACTCTAACCTCTACTTCCTACTCACATAGAGAGA
AGCCTGGTACTTTTTACCAACAAGAGTTACCAGAGAGTAACTTAACCGAA
GAGCCTTTGGAAGTTTCAGCTGCTCCTGGCCCAGTGGAGCAGAAGACGGG
AATACCTACAGTATCCTCTACATCCCACTCACATGGAGAGGACCTCCTCT
TTTTCTATCGACAGACCTTGCCAGATGGTCATCTAACTGATCAGGCTCTG
AAAGTCTCAGCTGTGTCTGGACCAGCTGACCAGAAGACTGGGACAGCAAC
AGTACTCTCTACTCCCCACTCACATAGAGAGAAGCCTGGTATTTTTTACC
AACAAGAGTTCGCAGACAGTCATCAAACTGAAGAGACTCTTACTAAAGTT
TCAGCCACTCCTGGACCAGCTGACCAGAAGACTGAGATACCAGCAGTACA
GTCTAGTTCTTACTCACAAAGAGAAAAGCCTAGTATTTTGTACCCACAGG
ACTTAGCAGACAGTCATCTACCTGAAGAGGGTCTGAAAGTTTCAGCTGTT
GCTGGACCAGCTGACCAGAAGACTGGCCTACCAACAGTACCCTCTAGTGC
ATACTCACACAGAGAGAAGCTCCTTGTTTTCTACCAACAGGCCTTGCTGG
ACAGCCATCTACCCGAAGAGGCTCTGAAAGTTTCAGCTGTTTCTGGACCA
GCTGACGGAAAGACTGGGACACCAGCTGTAACCTCTACTTCCTCTGCGTC
CTCTTCACTTGGAGAAAAGCCCAGTGCTTTCTATCAGCAGACCTTACCCA
ATAGTCATCTAACTGAAGAGGCTCTGAAAGTATCAATTGTTCCTGGACCA
GGTGATCAGAAGACTGGGATACCCTCAGCACCATCTAGTTTCTACTCACA
CAGAGAGAAGCCCATTATTTTTTCCCAGCAGACCCTGCCAGACTTTCTTT
TCCCTGAAGAAGCTCTGAAGGTTTCAGCTGTTTCTGTATTGGCTGCCCAG
AAGACTGGGACACCAACAGTGTCCTCTAATTCTCACTCACATAGCGAGAA
ATCTAGTGTTTTCTACCAGCAAGAGTTGCCAGACAGTGATCTACCTAGAG
AATCTCTGAAAATGTCTGCTATTCCTGGACTGACTGACCAGAAGACTGTC
CCAACACCAACAGTACCTTCAGGTTCCTTCTCACATAGAGAGAAGCCCAG
TATTTTCTATCAACAGGAGTGGCCAGATAGTTATGCAACTGAAAAGGCTC
TGAAAGTTTCAACTGGCCCTGGACCAGCTGACCAGAAGACTGAGATACCA
GCAGTACAGTCTAGTTCTTACCCACAGAGGGAGAAGCCTAGTGTTTTGTA
CCCACAGGTGTTATCAGACAGTCATCTACCTGAAGAGAGTCTGAAAGTTT
CAGCCTTCCCTGGACCAGCTGACCAGATGACTGACACACCAGCAGTACCG
TCTAC

Amino Acid Sequence

MEPEDLPWPGELEEEEEEEEEEEEEEAAAAAAANVDDVVVVEEVEEEAGR
ELDSDSHYGPQHLESIDDEEDEEAKAWLQAHPGRILPPLSPPQHRYSEGE
RTSLEKKTESWHCLPQEMDSSQTLDTSQTRFNVRTEDTEVTDFPSLEEGI
LTQSENQVKEPNRDLFCSPLLVIQDSFASPDLPLLTCLTQDQEFAPDSLF
HQSELSFAPLRGIPDKSEDTEWSSRPSEVSEALFQATAEVASDLASSRFS
VSQHPLIGSTAVGSQCPFLPSEQGNNEETISSVDELKIPKDCDRYDDLCS
YMSWKTRKDTQWPENNLADKDQVSVATSFDITDENIATKRSDHFDAACSY
GQYWTQEDSSKQAETYLTKGLQGKVESDVITLDGLNENAVVCSERVAELQ
RKPTRESEYHSSDLRMLRMSPDTVPKAPKHLKAGDTSKGGIAKVTQSNLK
SGITTTPVDSDIGSHLSLSLEDLSQLAVSSLETTTGQHTDTLNQKTLADT
HLTEETLKVTAIPEPADQKTATPTVLSSSHSHRGKPSIFYQQGLPDSHLT
EEALKVSAAPGLADQTTGMSTLTSTSYSHREKPGTFYQQELPESNLTEEP
LEVSAAPGPVEQKTGIPTVSSTSHSHGEDLLFFYRQTLPDGHLTDQALKV
SAVSGPADQKTGTATVLSTPHSHREKPGIFYQQEFADSHQTEETLTKVSA
TPGPADQKTEIPAVQSSSYSQREKPSILYPQDLADSHLPEEGLKVSAVAG
PADQKTGLPTVPSSAYSHREKLLVFYQQALLDSHLPEEALKVSAVSGPAD
GKTGTPAVTSTSSASSSLGEKPSAFYQQTLPNSHLTEEALKVSIVPGPGD
QKTGIPSAPSSFYSHREKPIIFSQQTLPDFLFPEEALKVSAVSVLAAQKT
GTPTVSSNSHSHSEKSSVFYQQELPDSDLPRESLKMSAIPGLTDQKTVPT
PTVPSGSFSHREKPSIFYQQEWPDSYATEKALKVSTGPGPADQKTEIPAV
QSSSYPQREKPSVLYPQVLSDSHLPEESLKVSAFPGPA

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU24655
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence cloned has a 3 residue deletion E18-E20 and a longer deletion at aa110-151. Also deleted is Pro526. Amino acid sequence shown aligns to aa1084 of ref sequence (excluding deletions). 3' AccI site to ligate to native Acc1 site in DU11890 insert at nt 3278 of NM_015120.4 ref sequence