cDNA Clone ZGRF1

• Synonyms: C4orf21
• Plasmid: pcDNA5D FRT/TO ZGRF1-GFP
• Parent Plasmid: pcDNA5D FRT/TO C-GFP
• Expressed: ZGRF1-GFP
• Genbank: NM_018392.4 GI:254826808 with silent change g5505a
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGAAAGCCAAGAATTTATTGTTCTATATACTCATCAAAAGATGAAGAAGTCAAAAGTGTGGCAAGATGGAATTCTGAAGATCACTCACTTAGGAAACAAAGCAATTTTATATGATGACAAAGGAGCATGTTTGGAGAGTCTGTTTCTTAAATGCCTTGAGGTGAAACCTGGAGATGACTTAGAAAGTGATCGATACTTAATCACAGTTGAAGAGGTTAAAGTTGCTGGAGCCATAGGTATTGTTAAGCAGAATGTCAATAAAGAAGCACCAGAGTTAAATTCAAGGACATTTATATCCTCTGGCCGATCTCTTGGATGTCAGCCCTCTGGCTTAAAAAGGAAGTTTACTGGTTTTCAAGGACCACGTCAGGTTCCAAAGAAAATGGTTATTATGGAAAGTGGTGAATCAGCTGCATCACATGAGGCTAAGAAAACTGGCCCTACTATTTTTTCTCCATTCTGCAGCATGCCTCCTTTGTTTCCTACTGTTGGCAAGAAAGATGTAAATAATATACTGGCAGACCCTGAGAACATTGTGACTTACAAGAACAGGGAGAGAAATGCCATGGATTTTTCTTCGGTTTTTTCTCCATCCTTCCAGATTAACCCAGAAGTGCTGTGTGAAGAAAATTATTTTTGCTCACCTGTCAATTCTGGAAATAAGCTTTCAGACTCTTTACTGACCAATGAGCCTGTGAAAAGAGATAGTTTGGCATCTCACTATTCAGGAGTTTCACAAAACATCAGAAGCAAAGCACAGATATTAGCTCTTCTGAAGTCCGAATCATCTAGTTCATGTGAGGAACTAAATTCTGAGATGACAGAGCATTTTCCTCAAAAACAACCACAAGGAAGTTTAAAAATTGCTACTAAACCAAAGTACCTAATTCAACAGGAAGAGTGTGCTGAGATGAAGAGCACAGAAAATTTATACTACCAGCATCAATCAGAAAATACCATGAGAAATAAAAGCCGGTGGGCCATGTATTTATCCTCACAGAGTTCACCTATACATTCTTCTACTGTAGATGGGAATGATACAGAAAGGAAACCCAAGGCCCAGGAAGATGATGTAAATTCTAATTTGAAAGACCTTTCATTACAAAAAATTATACAGTTCGTTGAAACGTATGCTGAAGAGAGGAAAAAGTATAATGTAGACCAGTCAGTCGGTAATAATGATCCATCCTGGAATCAGGAAGTAAAATTAGAAATTCCTTCATTCAATGAAAGCAGTAGCTTACAGGTTACTTGTAGCAGTGCAGAAAATGATGGTATATTATCAGAATCTGACATTCAAGAAGATAATAAAATACCTTTTAATCAAAATGACAAGGGGTGCATTAAAGGATCAGTTCTCATTAAAGAAAATGCTCAGGAGGTAAATACATGTGGAACACTGGAAAAGGAGTATGAACAATCAGAGTCATCTCTGCCAGAACTGAAACATCTCCAAATTGAATCTAGTAATAATTCTAGGATCTCTGATGACATTACAGACATGATTTCTGAAAGTAAAATGGATAATGAAAGTCTTAATAGTATTCATGAATCTCTGAGTAATGTAACACAACCATTTTTGGAGGTAACTTTTAATCTGAACAATTTTGAGACCAGTGACACTGAGGAGGAATCACAGGAAAGCAACAAAATTTCCCAGGATTCAGAGAGTTGGGTAAAGGACATTTTGGTTAATGATGGCAATTCATGTTTTCAAAAGAGGTCTGAGAATACAAACTGTGAAGAGATTGAGGGTGAACATTTGCCATTCTTAACATCTGTTAGTGACAAACCTACAGTGACATTTCCTGTTAAAGAGACTCTGCCATCACAGTTTTGTGATAAAACTTATGTGGGTTTTGACATGGGAATATGTAAAACTGAAAACACAGGAAAAGAAATAGAGGAGTATAGTGACACATTAAGCAATTTTGAATCTTTCAAGTGGACTGATGCTGTATACGGAGATAATAAAGAAGATGCTAATAAACCTATTCAAGAAGTCAGAATTAACTATGATTTTGCTTTACCCCCGAATAAATCTAAAGGTATAAACATGAATTTACATATTCCTCATATTCAAAACCAGATTGCTGAAAACAGTAATCTATTTTCAGAAGATGCTCAACCTCAGCCTTTTATTTTGGGAAGTGACTTAGACAAAAATGATGAACATGTTTTACCCTCAACTTCTAGTAGTGACAACAGTGTCCAACTATTAAATACCAATCAGAATCACTATGAATGTATTGCACTTGATAAATCAAATACCCACATTTCCAATTCTTTGTTTTACCCACTGGGAAAAAAGCATCTTATTTCCAAAGACACAGAAGCACATATATCTGAACCTGAAGATTTGGGAAAGATTAGAAGTCCACCCCCTGACCATGTTGAGGTTGAAACTGCCAGAGAAGGCAAACAATACTGGAATCCTAGAAATTCTTCAGAACTTTCTGGATTAGTAAATACCATTTCTATTTTAAAGTCGCTATGTGAACACAGTACTGCTTTAGACAGCTTGGAAATATTGAAAAAGAAAAATACTGTCTTTCAGCAAGGAACTCAACAGACGTATGAGCCAGATAGCCCTCCTGAAGTGAGGAAACCATTTATTACAGTAGTTTCACCAAAGTCTCCTCATCTGCACAAGGATTCTCAACAGATACTAAAAGAAGATGAAGTTGAACTAAGTGAACCACTTCAGTCTGTGCAGTTCTCTTCCTCGGGAAGTAAAGAAGAGACTGCTTTTCAAGCTGTTATTCCTAAACAAATAGAGAGAAAAACCTGTGACCCTAAGCCTGTTGAGTTTCAAGGACATCAAGTAAAAGGATCAGCTACTAGTGGTGTAATGGTCAGAGGACACAGCTCACAGCTAGGATGCAGTCAGTTTCCAGATAGCACTGAGTATGAAAACTTTATGACAGAGACACCAGAGTTACCTAGCACGTGTATGCAGATTGACTTCTTGCAGGTGACATCACCAGAAGAAAACATCTCTACATTGAGCCCTGTTTCTACCTTTTCTTTGAACTCAAGAGATGAAGACTTCATGGTAGAATTCTCTGAGACGTCCCTGAAAGCAAGAACTTTACCTGATGATCTTCATTTTCTCAACTTGGAGGGGATGAAAAAATCCCGTTCTCTGGAGAATGAGAACCTTCAAAGGCTTTCATTATTAAGTAGAACCCAGGTTCCACTTATTACTTTGCCACGTACTGATGGGCCACCTGACTTAGACTCTCATTCGTATATGATCAACTCTAACACATACGAGTCTTCTGGCTCCCCCATGCTCAATTTGTGTGAAAAGTCAGCAGTTCTTTCGTTTAGCATTGAGCCTGAGGACCAAAATGAAACCTTTTTCTCTGAAGAATCTAGGGAAGTGAATCCAGGGGATGTTTCACTTAATAATATATCTACTCAGAGCAAGTGGCTGAAATATCAAAACACATCCCAATGCAACGTGGCTACTCCAAACAGAGTTGATAAGAGAATAACTGATGGCTTCTTTGCTGAGGCTGTTTCTGGGATGCATTTTAGAGACACAAGTGAAAGACAGAGTGATGCTGTCAATGAAAGCTCTTTAGACTCTGTGCATTTGCAAATGATAAAAGGCATGCTCTATCAACAGCGGCAGGATTTTAGCAGTCAAGATTCGGTTTCCAGAAAGAAAGTACTTTCTCTGAATTTAAAGCAGACTTCTAAGACAGAGGAAATTAAAAATGTATTAGGAGGGTCTACCTGCTACAACTACAGTGTAAAGGATTTACAGGAGATAAGTGGCTCTGAGCTGTGCTTTCCAAGTGGGCAGAAAATAAAATCTGCTTATCTTCCCCAAAGGCAAATTCACATACCAGCTGTTTTTCAGTCTCCTGCTCATTATAAGCAGACTTTCACATCTTGCCTCATAGAACATCTAAATATATTGCTGTTTGGGTTAGCACAAAACCTGCAGAAAGCTCTTTCAAAAGTTGACATATCATTTTATACATCATTGAAGGGAGAGAAACTGAAAAACGCAGAAAATAATGTACCATCCTGCCATCATAGTCAACCTGCAAAACTTGTCATGGTTAAAAAGGAAGGTCCAAATAAGGGTCGTCTCTTTTATACATGTGATGGACCCAAAGCTGATCGATGTAAATTCTTTAAATGGCTTGAGGACGTGACTCCAGGATATTCAACACAGGAAGGAGCTCGACCTGGCATGGTTTTAAGTGATATTAAGAGTATTGGCTTATATTTAAGAAGTCAAAAGATACCACTTTATGAGGAATGCCAGCTTTTGGTGAGAAAAGGATTTGATTTTCAGAGAAAACAGTATGGCAAACTAAAGAAGTTTACTACTGTAAATCCTGAGTTTTATAATGAACCAAAAACCAAACTTTATCTTAAGCTAAGTCGGAAGGAAAGATCTTCAGCTTATAGCAAAAATGATCTTTGGGTGGTTTCAAAAACCCTAGACTTTGAGCTGGATACTTTTATCGCATGTAGTGCTTTCTTTGGACCATCATCTATCAATGAGATAGAAATACTGCCTTTGAAAGGCTATTTCCCTTCTAATTGGCCCACTAACATGGTTGTCCATGCGTTATTGGTTTGTAATGCTAGCACAGAACTGACTACTTTGAAAAACATTCAGGACTACTTTAATCCAGCTACTCTACCTCTAACACAGTACCTGTTAACAACGTCTTCGCCAACTATAGTTAGTAACAAAAGAGTCAGTAAGAGAAAATTTATCCCACCAGCCTTCACAAATGTCAGTACAAAATTTGAACTACTCAGCCTAGGAGCAACATTGAAGTTAGCTAGTGAGTTGATTCAGGTACACAAGTTAAACAAGGATCAAGCTACAGCTCTAATTCAAATAGCTCAAATGATGGCATCACATGAAAGCATTGAAGAAGTGAAGGAACTGCAAACTCATACCTTCCCTATCACAATCATACATGGTGTGTTTGGAGCAGGAAAGAGTTACTTGCTGGCAGTGGTGATTTTGTTCTTTGTACAGCTGTTTGAAAAGAGTGAAGCTCCCACCATTGGAAATGCAAGGCCGTGGAAACTTCTGATTTCTTCTTCTACTAATGTGGCTGTTGACAGAGTACTTCTTGGGCTTCTCAGTCTTGGATTTGAAAACTTTATCAGAGTTGGGAGTGTTAGGAAGATTGCCAAACCAATTTTACCTTATAGCTTGCATGCTGGCTCAGAAAATGAAAGTGAACAGTTAAAAGAACTACATGCACTAATGAAAGAAGACCTGACTCCTACGGAAAGAGTCTATGTGAGAAAAAGCATTGAGCAGCATAAACTGGGGACCAATAGAACCCTGCTGAAGCAGGTTCGAGTAGTTGGAGTTACCTGTGCAGCCTGCCCATTCCCATGCATGAATGATCTTAAATTTCCTGTAGTTGTGCTGGATGAGTGTAGTCAGATAACTGAACCGGCCTCTCTCCTTCCCATTGCAAGGTTTGAGTGTGAAAAGCTGATTCTTGTTGGAGATCCCAAACAGCTACCTCCTACTATTCAGGGTTCTGATGCAGCTCATGAAAATGGATTGGAACAAACTCTTTTTGATCGACTTTGCTTAATGGGTCACAAGCCAATTCTATTGAGAACTCAATACCGTTGTCATCCTGCAATCAGTGCTATTGCTAATGATCTGTTTTACAAAGGAGCCCTCATGAATGGTGTAACAGAAATAGAGCGGAGCCCTTTATTGGAATGGCTACCAACCCTGTGTTTTTATAATGTTAAAGGACTAGAACAGATAGAAAGAGATAACAGCTTTCATAATGTGGCAGAAGCTACGTTTACACTCAAGCTGATTCAATCACTGATTGCAAGTGGAATAGCAGGCTCTATGATTGGTGTGATAACATTATACAAATCCCAGATGTACAAGCTTTGTCATTTACTCAGTGCTGTGGACTTTCACCATCCTGATATTAAAACTGTGCAGGTGTCCACAGTAGATGCTTTTCAGGGAGCTGAAAAGGAGATCATTATTCTGTCCTGTGTAAGGACAAGACAAGTAGGATTCATTGATTCAGAAAAAAGAATGAATGTTGCATTGACTAGAGGAAAGAGGCATTTGTTGATTGTGGGAAATTTAGCCTGTTTGAGGAAAAATCAACTTTGGGGACGAGTGATCCAACACTGCGAAGGAAGGGAAGATGGATTGCAACATGCAAACCAGTATGAACCACAGCTGAACCATCTCCTTAAAGATTATTTTGAAAAACAAGTGGAAGAAAAACAGAAGAAAAAGAGTGAAAAAGAGAAATCTAAAGATAAATCTCATTCAGCGGCCGC

Amino Acid Sequence

MESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGACLESLFL
KCLEVKPGDDLESDRYLITVEEVKVAGAIGIVKQNVNKEAPELNSRTFIS
SGRSLGCQPSGLKRKFTGFQGPRQVPKKMVIMESGESAASHEAKKTGPTI
FSPFCSMPPLFPTVGKKDVNNILADPENIVTYKNRERNAMDFSSVFSPSF
QINPEVLCEENYFCSPVNSGNKLSDSLLTNEPVKRDSLASHYSGVSQNIR
SKAQILALLKSESSSSCEELNSEMTEHFPQKQPQGSLKIATKPKYLIQQE
ECAEMKSTENLYYQHQSENTMRNKSRWAMYLSSQSSPIHSSTVDGNDTER
KPKAQEDDVNSNLKDLSLQKIIQFVETYAEERKKYNVDQSVGNNDPSWNQ
EVKLEIPSFNESSSLQVTCSSAENDGILSESDIQEDNKIPFNQNDKGCIK
GSVLIKENAQEVNTCGTLEKEYEQSESSLPELKHLQIESSNNSRISDDIT
DMISESKMDNESLNSIHESLSNVTQPFLEVTFNLNNFETSDTEEESQESN
KISQDSESWVKDILVNDGNSCFQKRSENTNCEEIEGEHLPFLTSVSDKPT
VTFPVKETLPSQFCDKTYVGFDMGICKTENTGKEIEEYSDTLSNFESFKW
TDAVYGDNKEDANKPIQEVRINYDFALPPNKSKGINMNLHIPHIQNQIAE
NSNLFSEDAQPQPFILGSDLDKNDEHVLPSTSSSDNSVQLLNTNQNHYEC
IALDKSNTHISNSLFYPLGKKHLISKDTEAHISEPEDLGKIRSPPPDHVE
VETAREGKQYWNPRNSSELSGLVNTISILKSLCEHSTALDSLEILKKKNT
VFQQGTQQTYEPDSPPEVRKPFITVVSPKSPHLHKDSQQILKEDEVELSE
PLQSVQFSSSGSKEETAFQAVIPKQIERKTCDPKPVEFQGHQVKGSATSG
VMVRGHSSQLGCSQFPDSTEYENFMTETPELPSTCMQIDFLQVTSPEENI
STLSPVSTFSLNSRDEDFMVEFSETSLKARTLPDDLHFLNLEGMKKSRSL
ENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPML
NLCEKSAVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKWLKYQ
NTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSV
HLQMIKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTC
YNYSVKDLQEISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTF
TSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCH
HSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEG
ARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFT
TVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACS
AFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQD
YFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLG
ATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPIT
IIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVA
VDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHA
LMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMND
LKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAA
HENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGV
TEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIA
SGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAE
KEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWG
RVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKD
KSHSAAAMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGY
VQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLE
YNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGP
VLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49980
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Recommend propagation at 30 degrees.