cDNA Clone ISG15

• Plasmid: pGEX6P-1-ISG15 1-157 C78S
• Parent Plasmid: pGEX6P1
• Expressed: GST ISG15 1-157 C78S
• Genbank: NP_005092
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGGCTGGGACCTGACGGTGAAGATGCTGGCGGGCAACGAATT
CCAGGTGTCCCTGAGCAGCTCCATGTCGGTGTCAGAGCTGAAGGCGCAGA
TCACCCAGAAGATCGGCGTGCACGCCTTCCAGCAGCGTCTGGCTGTCCAC
CCGAGCGGTGTGGCGCTGCAGGACAGGGTCCCCCTTGCCAGCCAGGGCCT
GGGCCCCGGCAGCACGGTCCTGCTGGTGGTGGACAAATCCGACGAACCTC
TGAACATCCTGGTGAGGAATAACAAGGGCCGCAGCAGCACCTACGAGGTG
CGGCTGACGCAGACCGTGGCCCACCTGAAGCAGCAAGTGAGCGGGCTGGA
GGGTGTGCAGGACGACCTGTTCTGGCTGACCTTCGAGGGGAAGCCCCTGG
AGGACCAGCTCCCGCTGGGGGAGTACGGCCTCAAGCCCCTGAGCACCGTG
TTCATGAATCTGCGCCTGCGGGGAGGCTAAACAGAGCCTGGCGGGGAGGC
TAAACAGAGCCTGGCGGGGAGGCTAAACAGAGCCTGGCGGGGAGGCTAAA
CAGAGCCTGGCGGGCGGAGCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGWDLTVKMLAGNEFQVSL
SSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGS
TVLLVVDKSDEPLNILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQD
DLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24665
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 3' Not1 site 100bp downstream of stop codon