cDNA Clone DCUN1D1

• Plasmid: pET15b 6His 3C- DCUN1D1-Ub (KallR)
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-DCUN1D1-Ubiquitin (KallR)
• Genbank: NM_020640
• Tag: 6His
• Species: Human

Sequence of Insert

gcggccgctggcggctccggcggaggttccggaATGCAGATATTCGTGAG
GACCCTGACTGGTAGGACCATCACTCTCGAAGTGGAGCCGAGTGACACCA
TTGAGAATGTCAGGGCAAGGATCCAAGACAGGGAAGGCATCCCTCCTGAC
CAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGGACGCACCCT
GTCTGACTACAACATCCAGAGAGAGTCCACCCTGCACCTGGTCCTCCGTC
TCAGAgctgcgTGAgcggccgc

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMNKLKSSQKDKVRQFMIFTQSSEKTAV
SCLSQNDWKLDVATDNFFQNPELYIRESVKGSLDRKKLEQLYNRYKDPQD
ENKIGIDGIQQFCDDLALDPASISVLIIAWKFRAATQCEFSKQEFMDGMT
ELGCDSIEKLKAQIPKMEQELKEPGRFKDFYQFTFNFAKNPGQKGLDLEM
AIAYWNLVLNGRFKFLDLWNKFLLEHHKRSIPKDTWNLLLDFSTMIADDM
SNYDEEGAWPVLIDDFVEFARPQIAGTKSTTVAAAGGSGGGSGMQIFVRT
LTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIFAGRQLEDGRTLS
DYNIQRESTLHLVLRLRAA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24684
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: DCUN1D1 carries g318a mutation (silent Ala106). vector 24673