cDNA Clone FAN1 (mouse)

• Synonyms: 6030441H18Rik; Mtmr15
• Plasmid: pGEX6P1 mouse FAN1 C44/47A
• Parent Plasmid: pGEX6P1
• Expressed: GST-FAN1m C44/47A
• Genbank: NM_177893.3 with silent change a414g
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGCCGTCACAAAGGAAATCACCTGATCAAAAAAGACCCCGTAGAAGCTTATCTACCAGCAAAACTGCAAAAAGCCAGTGTCATTCGATTACTTCGTATTTTAACAGTGCACCACCTGCTAAACTTGCAGCTTCAACTGCTCATAAAATGGTGCCCAGGTATGACCTCATTCGGCACCTTGATGAGTCTTGTGCTAACAACGGTGTTGGTGATGACGTTCAAGTAGAGCCTGCTCAGGCTGGATTAATGAGTCCAACTGTGCCTACATCAGATTTACCCAGTGGTCCTTTAGAGAATGTGACACCTCAGAAATTATCACCACCAAAGAGAAGTTTAATTTCTGTCCAATGTGGTTCAAAACTGGGCATACAACAGCAGACCAGTCCCTACTTTAAAGATGCCTTAGTGTCCAAGGATCAAAATGAGCTTCCAAATCAGAGTGTAGAAATTATGCCTTTGGGAAGTCTGACATCGAAATTGTCCAGACGATACTTAAACGCTAAAAAATCACTCGCTAAGAATGAGGGACTAGCCAGTCAGTGCCCACAGACTTCTCCGTCCACACCTGGCACCAGCCTGACTGATAACTGTCCAGAGATGGAAGACAAAGATGAGGTTTTGAACAGTTCCCAAAAGGAAAACATTTATTCATGTGCACCTCTAAAAGAAGAGAATGCTTCAGAACAGAAGGTAAAGAACAATAAAATAACGGGAGATGAAAGCCAGAAAGCTTCCTGCGGGGAGCCAGCCCTCACCCCTGCATCTGCAGAGCATGCTTCCATATTACTGTCCTCAGATTCAACTCTCGTCAGTAACACGAAATCCTCTCCGGGAGACACTCTTGTAAAGCAAGAAAGTGCCAGAAGGGCAGATGTAGGGCTTGCTGAGCCGCTCGAGGTGCGCAGTCACAAAGAAGTACAAATGACTTTCGATGCTGCGGCCAAAACGCTGGTGTCTGGGGAGGCAGAATCAAATGGTCCTACCGATGTTGACATGTCTGACATGACTACGTGGAGTAACAACCAAGAGCTTGTCAGGGAAGCTGGCAGTGTCTTACACTGCCCTCTGGAGCAGGGGTCCAGCTGCGGTGGCCCCAGTGAGACAGCTCAACTGGCACTGAGTCACCCGTACTACCTGCGGAGCTTCCTGGTGGTGCTGCAGGCCCTCCTTGGGAACGAAGAGGACATGAAGCTCTTCGATGAGCAGGAGAAGGCGATTATCACCAGGTTTTACCAGTTGTCAGCTAGTGGTCAAAAGTTGTATGTGAGGCTCTTTCAACGTAAATTAACCTGGATTAAAATGAGTAAACTGGAGTATGAAGAGATTGCCTCCGACTTAACCCCTGTGGTTGAAGAATTGAAGGACTCAGGCTTTCTACAGACAGAATCTGAGTTGCAAGAACTCTCTGATGTACTTGAACTCCTTTCTGCTCCTGAGCTGAAAGCCCTGGCCAAAACCTTCCACTTGGTGAGTCCCGGTGGGCAGAAGCAGCAGCTGGTAGACGCCTTTCACAAACTGGCCAAACAGCGCTCAGTCTGCACGTGGGGCAAGACTCAGCCTGGAATCCGAGCAGTGATTTTAAAAAGAGCCAAAGACTTGGCTGGCCGGTCGCTTCGAGTCTGTAAAGGCCCTAGGGCTGTGTTTGCCCGAATCTTGCTTCTGTTCTCATTGACTGATTCCATGGAAGACGAAGAAGCTGCTTGTGGGGGTCAAGGTCAGCTGTCTACTGTGCTATTGGTCAATCTGGGCCGAATGGAGTTTCCTCAGTACACCATCTGCCGGAAGACCCAGATCTTCAGGGACAGAGAGGACCTCATCAGATACGCAGCAGCCGCACACATGCTGAGTGACATCTCAGCTGCCATGGCCAGTGGGAACTGGGAGGACGCTAAGGAGCTTGCTCGGAGTGCAAAAAGGGACTGGGAACAACTGAAAAGCCACCCTTCCCTGAGGTACCACGAGGCCTTGCCGCCCTTCCTGCGCTGCTTTACCGTCGGGTGGATCTACACAAGGATTTCCTCTCGGGCTGTTGAAGTCCTAGAGAGGCTTCACATGTACGAGGAAGCTGTCAAGGAACTTGAAAACCTCTTGTCTCAGAAAATCTATTGTCCTGACAGCAGAGGCCGCTGGTGGGATCGCCTGGCTCTTAACCTGCACCAGCACCTGAAGCGTCTGGAAGAGGCAATTAGGTGCATCAGGGAAGGCCTGGCAGACCCACACGTGAGGACAGGACACCGACTTTCTCTTTATCAGCGAGCCGTGCGCCTGCGAGAGTCTCCGAGCTGCAGGAAGTACAAGCACCTCTTCAGCCGGCTGCCCGAGGTTGCCGTGGGAGACGTCAAGCATGTGACCATCACGGGCAGGCTGTGCCCACAGCATGGCATGGGCAAGTCTGTGTTTGTCATGGAGAGTGGAGACGGCGCCAACCCCACCACAGTCCTGTGTTCTGTGGAGGAGCTGGCCCTGGGTTATTACAGGCAGAGTGGCTTTGACCAAGGGATTCATGGAGAAGGGTCCACCTTCAGCACCCTGTGTGGCCTCCTGCTGTGGGATATCATCTTCATGGATGGAATACCAGACGTCTTCAGAAATGCCTACCAGGCATCCCCACTGGATTTGCTCACGGACAGCTTCTTCGCAAGCAGGGAGCAGGCCCTGGAAGCCAGGCTGCAGCTGATCCACAGCGCCCCTGCTGAGAGCCTGCGGGCCTGGGTGGGCGAAGCATGGCAGGCCCAGCAAGGCAGAGTGGCCTCTCTGGTCAGCTGGGACCGCTTCACCTCCCTACAGCAAGCTCAGGATCTTGTCTCCTGCCTCGGGGGTCCTGTCCTCAGTGGTGTGTGCAGGCGCCTGGCTGCTGACTTTCGGCACTGCCGAGGGGGCCTCCCAGACTTGGTGGTGTGGAATTCTCAGAGCCACCATTGCAAGCTGGTGGAGGTGAAAGGCCCCAGTGATCGACTTTCATGTAAGCAGATGATCTGGCTGTACGAACTCCAAAAGCTGGGGGCTGATGTAGAAGTGTGCCATGTGGTTGCAGTTGGTGCTAAGAGCAAAGGTCTTGGCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMPSQRKSPDQKRPRRSLST
SKTAKSQCHSITSYFNSAPPAKLAASTAHKMVPRYDLIRHLDESCANNGV
GDDVQVEPAQAGLMSPTVPTSDLPSGPLENVTPQKLSPPKRSLISVQCGS
KLGIQQQTSPYFKDALVSKDQNELPNQSVEIMPLGSLTSKLSRRYLNAKK
SLAKNEGLASQCPQTSPSTPGTSLTDNCPEMEDKDEVLNSSQKENIYSCA
PLKEENASEQKVKNNKITGDESQKASCGEPALTPASAEHASILLSSDSTL
VSNTKSSPGDTLVKQESARRADVGLAEPLEVRSHKEVQMTFDAAAKTLVS
GEAESNGPTDVDMSDMTTWSNNQELVREAGSVLHCPLEQGSSCGGPSETA
QLALSHPYYLRSFLVVLQALLGNEEDMKLFDEQEKAIITRFYQLSASGQK
LYVRLFQRKLTWIKMSKLEYEEIASDLTPVVEELKDSGFLQTESELQELS
DVLELLSAPELKALAKTFHLVSPGGQKQQLVDAFHKLAKQRSVCTWGKTQ
PGIRAVILKRAKDLAGRSLRVCKGPRAVFARILLLFSLTDSMEDEEAACG
GQGQLSTVLLVNLGRMEFPQYTICRKTQIFRDREDLIRYAAAAHMLSDIS
AAMASGNWEDAKELARSAKRDWEQLKSHPSLRYHEALPPFLRCFTVGWIY
TRISSRAVEVLERLHMYEEAVKELENLLSQKIYCPDSRGRWWDRLALNLH
QHLKRLEEAIRCIREGLADPHVRTGHRLSLYQRAVRLRESPSCRKYKHLF
SRLPEVAVGDVKHVTITGRLCPQHGMGKSVFVMESGDGANPTTVLCSVEE
LALGYYRQSGFDQGIHGEGSTFSTLCGLLLWDIIFMDGIPDVFRNAYQAS
PLDLLTDSFFASREQALEARLQLIHSAPAESLRAWVGEAWQAQQGRVASL
VSWDRFTSLQQAQDLVSCLGGPVLSGVCRRLAADFRHCRGGLPDLVVWNS
QSHHCKLVEVKGPSDRLSCKQMIWLYELQKLGADVEVCHVVAVGAKSKGL
G*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU50108
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.