cDNA Clone human SV2C

• Plasmid: pcDNA5D frtTO FLAG SV2C T42A S45/47A
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: FLAG-SV2C T42A S45/47A
• Genbank: NM_014979.1
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCGATGGAAGACTCTTACAAGGATAGGACTTCACTGATGAAGGGTGCCAAGGACATTGCCAGAGAGGTGAAGAAACAAACAGTAAAGAAGGTGAATCAAGCTGTGGACCGAGCCCAGGATGAATACGCCCAGAGGGCCTACGCTCGGTTCCAAGATGAAGAAGATGATGATGACTACTACCCGGCTGGAGAAACCTATAATGGTGAGGCCAACGATGACGAAGGCTCAAGTGAAGCCACTGAGGGGCATGATGAAGATGATGAGATCTATGAGGGGGAGTATCAGGGCATCCCCAGTATGAACCAAGCGAAGGACAGCATCGTGTCAGTGGGGCAGCCCAAGGGCGATGAGTACAAGGACCGGCGGGAGCTGGAATCAGAAAGGAGAGCTGACGAGGAAGAGTTAGCCCAGCAGTATGAGCTGATAATCCAAGAATGCGGTCATGGTCGTTTTCAGTGGGCCCTTTTCTTCGTCCTGGGCATGGCTCTTATGGCAGACGGTGTAGAGGTGTTTGTCGTTGGCTTCGTGTTACCCAGTGCTGAGACAGACCTCTGCATCCCAAATTCAGGATCTGGATGGCTAGGCAGCATAGTGTACCTCGGGATGATGGTGGGGGCGTTCTTCTGGGGAGGACTGGCAGACAAAGTGGGAAGGAAACAGTCTCTTCTGATTTGCATGTCTGTCAACGGATTCTTTGCCTTCCTTTCTTCATTTGTCCAAGGTTATGGCTTCTTTCTCTTCTGTCGCTTACTTTCTGGATTCGGGATTGGAGGAGCCATACCCACTGTGTTCTCGTACTTTGCTGAAGTCCTGGCCCGGGAAAAGCGGGGCGAACACTTGAGCTGGCTCTGCATGTTCTGGATGATCGGTGGCATCTACGCCTCTGCCATGGCCTGGGCCATCATCCCGCACTACGGGTGGAGCTTCAGCATGGGATCGGCCTACCAGTTTCACAGTTGGCGTGTGTTTGTCATCGTCTGTGCACTCCCCTGTGTCTCCTCCGTGGTGGCCCTCACATTCATGCCTGAAAGCCCACGATTCTTGTTGGAGGTTGGAAAACATGATGAAGCTTGGATGATTCTGAAGTTAATTCATGACACCAACATGAGAGCCCGGGGTCAGCCTGAGAAGGTCTTCACGGTAAACAAAATAAAAACTCCTAAACAAATAGATGAGCTGATTGAAATTGAGAGTGACACAGGAACATGGTATAGGAGGTGTTTTGTTCGGATCCGCACCGAGCTGTACGGAATTTGGTTGACTTTTATGAGATGTTTCAACTACCCAGTCAGGGATAATACAATAAAGCTTACAATTGTTTGGTTCACCCTGTCCTTTGGGTACTATGGATTATCCGTTTGGTTCCCTGATGTCATTAAACCTCTGCAGTCCGATGAATATGCATTGCTAACCAGAAATGTGGAGAGAGATAAATATGCAAATTTCACTATTAACTTTACAATGGAAAATCAGATTCATACTGGAATGGAATACGACAATGGCAGATTCATAGGGGTCAAGTTCAAATCTGTAACTTTCAAAGACTCTGTTTTTAAGTCCTGCACCTTTGAGGATGTAACTTCAGTGAACACCTACTTCAAGAACTGCACATTTATTGACACTGTTTTTGACAACACAGATTTTGAGCCATATAAATTCATTGACAGTGAATTTAAAAACTGCTCGTTTTTTCACAACAAGACGGGATGTCAGATTACCTTTGATGATGACTATAGTGCCTACTGGATTTATTTTGTCAACTTTCTGGGGACATTGGCAGTATTGCCAGGGAACATTGTGTCTGCTCTGCTGATGGACAGAATTGGGCGCTTAACAATGCTAGGTGGCTCTATGGTGCTTTCGGGGATCAGCTGTTTCTTCCTTTGGTTCGGCACCAGTGAATCCATGATGATAGGCATGCTGTGTCTGTACAATGGATTGACCATCTCAGCCTGGAACTCTCTTGACGTGGTCACTGTGGAACTGTACCCCACAGACCGGAGGGCAACAGGCTTTGGCTTCTTAAATGCGCTATGCAAGGCAGCAGCCGTCCTGGGAAACTTAATATTTGGCTCTCTGGTCAGCATCACCAAATCAATCCCCATCCTGCTGGCTTCTACTGTGCTCGTGTGTGGAGGACTCGTTGGGCTGTGCCTGCCTGACACACGAACCCAGGTTCTGATGTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMEDSYKDRTSLMKGAKDIAREVKKQTVK
KVNQAVDRAQDEYAQRAYARFQDEEDDDDYYPAGETYNGEANDDEGSSEA
TEGHDEDDEIYEGEYQGIPSMNQAKDSIVSVGQPKGDEYKDRRELESERR
ADEEELAQQYELIIQECGHGRFQWALFFVLGMALMADGVEVFVVGFVLPS
AETDLCIPNSGSGWLGSIVYLGMMVGAFFWGGLADKVGRKQSLLICMSVN
GFFAFLSSFVQGYGFFLFCRLLSGFGIGGAIPTVFSYFAEVLAREKRGEH
LSWLCMFWMIGGIYASAMAWAIIPHYGWSFSMGSAYQFHSWRVFVIVCAL
PCVSSVVALTFMPESPRFLLEVGKHDEAWMILKLIHDTNMRARGQPEKVF
TVNKIKTPKQIDELIEIESDTGTWYRRCFVRIRTELYGIWLTFMRCFNYP
VRDNTIKLTIVWFTLSFGYYGLSVWFPDVIKPLQSDEYALLTRNVERDKY
ANFTINFTMENQIHTGMEYDNGRFIGVKFKSVTFKDSVFKSCTFEDVTSV
NTYFKNCTFIDTVFDNTDFEPYKFIDSEFKNCSFFHNKTGCQITFDDDYS
AYWIYFVNFLGTLAVLPGNIVSALLMDRIGRLTMLGGSMVLSGISCFFLW
FGTSESMMIGMLCLYNGLTISAWNSLDVVTVELYPTDRRATGFGFLNALC
KAAAVLGNLIFGSLVSITKSIPILLASTVLVCGGLVGLCLPDTRTQVLM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48621
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: T42A, S45A and S47A. Also contains a single silent nucleotide change A>G 360 bp.