cDNA Clone SGK3

• Synonyms: CISK, SGKL
• Plasmid: pSCB SGK3 Cter donor Sal Xho
• Parent Plasmid: pSC-B
• Genbank: ENSG00000104205
• Species: Human

Sequence of Insert

GTCGACTAAAGGACTAAATTGGGCCAGGCGCGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGAATCACAAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGATGAAACCCTGTCTGTACTAAAAATACAAAAAGTTAGCTGGGCGTGGTAGGTGGGCGCCTGTAATCCCAGCTACTCGGGAGGGTGAGGCAGGAGAATTGCTTGAACCCAGGAGGCGGAGGTTGCAGTGAGCCAAGATCGCGCCACTGGCATGCCAGCCCAGGTGGCAGTGTGAGACTCTGTCTCAAATAAATAAATAAATAAAGGATTAAATTAATGAAAGAAAAGCTAAGGTGAATAATACTGTGCTTTTGATGTTTTAGGCTGGACCAGATGATATCAGAAACTTTGACACAGCATTTACAGAAGAAACAGTTCCATATTCTGTGTGTGTATCTTCTGACTATTCTATAGTGAATGCCAGTGTATTGGAAGCTGACGACGCGTTCGTTGGTTTCTCTTATGCACCTCCTTCAGAAGACTTATTTTTGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTGAGCAGTTTGCCATTCAGAAACCATTGAGCAAAATAAGTCTATAGATGGGACTGAAACTTCTATTTGTGTGAATATATTCAAATATGTATAACTAGTGCCTCATTTTTATATGTAATGATGAAAACTATGAAAAAATGTATTTTCTTCTATGTGCAAGAAAAATAGGGCATTTCAAAGAGCTGTTTTGATTAAAATTTATATTCTTGTTTAATAAGCTTATTTTTAAACAATTTAAAAGCTATTATTCTTAGCATTAACCTATTTTTAAAGAAACCTTTTTTGCTATTGACTGTTTTTTCCCTCTAAGTTTACACTAACATCTACCCAAGATAGACTGTTTTTTAACAGTCAATTTCAGTTCAGCTAACATATATTAATACCTTTGTAACTCTTTGCTATGGCTTTTGTTATCACACCAAAACTATGCAATTGGTACATGGTTGTTTAAGAAGAAACCGTATTTTTCCATGATAAATCACTGTTTGAAATATTTGGTTCCTCGAG

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU48625
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Designed to insert an in-frame GFP upstream of the SGK3 stop codon. Targets last coding exon (exon 17). Complete sequence of donor including homology arms shown as sequence of insert, contains several silent mutations to prevent Cas9 targeting the donor sequence. Sequence of cassette depicts the expressed SGK3-GFP fusion.