cDNA Clone SLC12A2

• Synonyms: BSC, BSC2, NKCC1, PPP1R141
• Plasmid: pMK-RQ SLC12A2 Nter GFP donor
• Parent Plasmid: pMK-RQ
• Species: Human

Sequence of Insert

gcggccgcCGCTCCTTTTCGCCGCAGAGCGCGGCGGGGGAGGGAGGTGAAGGCGTGGGAGGAAGGCGGCGGAAGAGGAGGTAGCCGCGCGGAGATCCTCCCGGCGACATCACTGAGGCGGAGCGTAGCCGCGCCTGCTCCCCGATCGCGCCGTTCGTGCTGCCCGCACTGCCCGAGGCCCTTTGCGGACTGGCTGGCCGCGCTCTTCGCACGGGGCGCTTTTGCGTGGGGTCGCGCAGGAGCCGCGGAGGGCCGGATCGCTGAGGGAGCGTCAGCCGCGCGCCGCTGCGGGACCGCGGGTGGGCGGGCTGAGGGGCGGAGGAGCCTCCGGCCCCGCCTCCCGCGTGCTGCTGCCGGAGCCGCCGCCGCCGCAGCCCCGCCGAGGCTTTAAACAGCGGGGCCCGCCCCGCGCCCGCCACACTCGCGCGCTCGCTCGGCTGCCGGTGGCCTCTGTGGCCGTCCAGGCTAGCGGCGGCCCGCAGGCGGCGGGGAGAAAGACTCTCTCACCTGGTCTTGCGGCTGTGGCCACCGCCGGCCAGGGGTGTGGAGGGCGTGCTGCCGGAGACGTCCGCCGGGCTCTGCAGTTCCGCCGGGGGTCGGGCAGCTATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGGAGCCGCGGCCCACtGCaCCgagtagcGGtGCaCCtGGACTGGCCGGGGTCGGGGAGACGCCGTCAGCCGCTGCGCTGGCCGCAGCCAGGGTGGAACTGCCCGGCACGGCTGTGCCCTCGGTGCCGGAGGATGCTGCGCCCGCGAGCCGGGACGGCGGCGGGGTCCGCGATGAGGGCCCCGCGGCGGCCGGGGACGGGCTGGGCAGACCCTTGGGGCCCACCCCGAGCCAGAGCCGTTTCCAGGTGGACCTGGTTTCCGAGAACGCCGGGCGGGCCGCTGCTGCGGCGGCGGCGGCGGCGGCGGCAGCGGCGGCGGCTGGTGCTGGGGCGGGGGCCAAGCAGACCCCCGCGGACGGGGAAGCCAGCGGCGAGAGCGAGCCGGCTAAAGGCAGCGAGGAAGCCAAGGGCCGCTTCCGCGTGAACTTCGTGGACCCAGCTGCCTCCTCGTCGGCTGAAGACAGCCTGTCAGATGCTGCCGGGGTCGGAGTCGACGGGCCCAACGTGAGCTTCCAGAACGGCGGGGACACGGTGCTGAGCGAGGGCAGCAGCCTGCACTCCGGCGGCGGCGGCGGCAGTGGGCACCACCAGCACTACTATTATGATACCCACACCAACACCTACTACCTGCGCACCTTCgcggccgc

• Vector Type: Other
• Antibiotic: Kan
• DU Number: DU48658
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR donor to be used in conjunction with single guides 48609-48611 (individually) and wild-type Cas9. Introduces an in-frame GFP to the n-terminus, sequence of insert shows the GFP plus flanking homology arms.