cDNA Clone OSR1

• Synonyms: ODD, OXSR1
• Plasmid: pMK-RQ OSR1 Nter GFP donor
• Parent Plasmid: pGEX6P-1
• Genbank: NP_005100
• Species: Human

Sequence of Insert

gcggccgcGTGTTGTACTATAAAACATTGGTTGTCCCAGTCTTTGCACCTTGTCAATTCTGCCTCAGAATGTCTGAATCCACGTGTGCAATTCTGTGTTTATCTGCAAATAAATGTGTAAGTATGTTCCCCAGCTCCTGTTATCTTTGCAGGAGATTGTGATTCTGTGTTGACCCGGGCTTACCTGGATGTCAATTTCTACAGGACAGGCAGTTTCCCTTTGCTTGTTCCTTTCTCAGTGTCTTGCATACAGTAGGCCCTTCCCTCTATCAAGTTGTGTGGCTCTGCCAGACGCCATTTGGAGGGAGCCCTTAGACCAAGGAGGGTTCTGAAAAGAGGGCTCCCCTGCGTCTTACATTCCCACCCTCTTTGTGAGGGCAGTGGGTTTTGGGAATGGGGTCTTAGACTCTTAGCTCCTGCGCTGGGTGTGAGGAGAGGGTGTGGCTCGGCTGGAATTCAGGAGGAAGGCGACCCACCCTGAACTTCTTTATTCCTCTCCACGCCCCTGCCTTCTTCTTTTCTTTGCAGATCCGGATTGAGAAGCCACTGCAACTACCGAAATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGGGCAGCAAAACCTTGCCtGCtCCaGTaCCgATaCAtCCaagtCTGCAGCTCACCAAtTAtagtTTCtTgCAaGCAGTGAACGGCCTGCCCACAGTGCCTTCGGACCATCTGCCCAACCTGTATGGTTTCAGCGCGTTGCACGCTGTGCACCTGCATCAGTGGACGCTGGGCTACCCGGCCATGCACTTGCCGCGCTCTTCTTTCTCCAAAGTGCCGGGCACGGTGTCCAGCTTGGTGGATGCGCGCTTCCAGCTGCCCGCCTTTCCCTGGTTCCCTCATGTCATTCAACCCAAGCCCGAGATCACCGCTGGAGGCAGCGTTCCAGCGCTCAAGACCAAGCCGCGCTTTGATTTTGCCAACCTGGCCTTGGCAGCAACGCAAGAAGATCCGGCCAAGCTCGGTCGCGGGGAGGGCCCAGGCTCCCCTGCAGGTGGGCTGGGTGCCCTCCTCGACGTGACCAAGCTGTCTCCAGAAAAGAAGCCCACAAGGGGACGTCTGCCTTCCAAGACCAAGAAGGAATTCGTCTGCAAGTTCTGTGGCCGCCACTTCACCAAGTCCTACAACCTACTTATCCATGAGCGGACGCACACCGACGAGCGGCCCTACACCTGTGACATCTGCCACAAAGCCTTCCGGAGGCAAGACCACCTGCGAGACCACAGGTGCGGCACTGCGGGTGGCGCAAGAGAGAGCTCTCTGCCTCTGGCCTCACCATgcggccgc

• Vector Type: Other
• Antibiotic: Kan
• DU Number: DU48716
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR donor to introduce an in-frame GFP to the N-terminus of OSR1. To be co-transfected with pairs: 48766/48770 or 48767/48765 (D10A strategy). Sequence of insert shows GFP with flanking homology arms, several silent mutations are present to prevent attack by cas9.