cDNA Clone CCDC99

• Synonyms: SPDL1, Spindly
• Plasmid: pcDNA5D frtTO GFP CCDC99
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP-CCDC99
• Genbank: NM_017785.4
• Tag: GFP
• Species: Human

Sequence of Insert

GAATTCATGGAGGCAGATATAATCACAAATCTTCGATGCAGGCTCAAAGAGGCTGAAGAAGAGCGACTAAAAGCTGCACAGTATGGTTTACAACTAGTAGAGAGTCAAAATGAATTACAGAATCAATTGGATAAATGTCGTAATGAAATGATGACCATGACTGAGAGTTATGAACAAGAAAAATATACCCTTCAAAGAGAAGTTGAACTCAAGAGTCGAATGTTAGAAAGTTTGAGCTGCGAATGTGAAGCTATTAAACAACAACAAAAAATGCACCTGGAGAAATTGGAAGAACAACTAAGCAGAAGCCATGGACAGGAAGTGAATGAACTAAAAACTAAGATAGAAAAACTGAAAGTGGAATTAGATGAAGCCAGGCTTAGTGAAAAGCAGCTGAAGCACCAAGTAGATCATCAGAAGGAACTCCTCTCTTGTAAATCAGAGGAACTGCGCGTAATGTCTGAACGTGTGCAGGAAAGCATGTCTTCAGAGATGCTGGCTCTTCAAATTGAGCTGACAGAAATGGAGAGTATGAAGACCACCCTCAAAGAAGAAGTGAATGAACTACAATACAGACAAGAACAGCTAGAACTTCTTATTACTAACCTAATGCGCCAGGTAGACCGGCTTAAAGAGGAAAAAGAGGAGCGAGAGAAAGAAGCAGTTTCTTACTATAATGCCCTAGAGAAAGCTCGTGTAGCAAATCAAGATCTTCAGGTACAGTTGGACCAGGCACTCCAGCAAGCCTTGGATCCCAATAGTAAAGGCAACTCTTTGTTTGCAGAGGTGGAAGATCGAAGGGCAGCAATGGAACGTCAGCTTATCAGTATGAAAGTCAAGTATCAGTCACTAAAGAAGCAAAATGTATTTAACAGAGAACAGATGCAGAGAATGAAGTTACAAATTGCCACGTTGCTACAGATGAAAGGGTCTCAAACTGAATTTGAGCAGCAGGAACGGTTGCTTGCCATGTTGGAGCAGAAGAATGGTGAAATAAAACATCTTTTAGGTGAAATTAGAAATCTGGAGAAATTTAAGAATTTATATGACAGTATGGAATCTAAGCCTTCAGTCGACTCTGGTACTCTGGAAGATAACACCTATTATACAGATTTACTTCAGATGAAGCTGGATAACTTAAACAAAGAAATTGAAAGCACTAAAGGTGAATTGTCCATACAGCGAATGAAAGCATTATTTGAGAGCCAGCGGGCTCTAGATATTGAGCGAAAACTTTTTGCAAATGAAAGATGCCTCCAGCTTTCAGAAAGTGAAAATATGAAACTGAGAGCTAAACTAGATGAATTGAAACTAAAATATGAACCTGAAGAGACAGTTGAAGTGCCTGTACTGAAAAAGAGGCGTGAGGTGCTCCCTGTGGATATAACCACCGCTAAAGATGCATGTGTCAACAACAGTGCTCTCGGGGGAGAAGTTTATCGATTACCGCCTCAGAAAGAGGAGACACAGTCCTGCCCTAACAGTTTAGAAGATAACAACTTGCAATTAGAAAAATCAGTTTCTATACACACACCAGTAGTCAGTCTCTCTCCTCACAAAAATCTGCCCGTGGATATGCAGCTGAAGAAGGAAAAGAAATGTGTGAAACTCATAGGAGTTCCCGCTGACGCTGAGGCCTTAAGTGAAAGAAGTGGAAACACCCCTAACTCTCCCAGGTTAGCTGCTGAATCAAAGCTTCAAACAGAAGTTAAAGAAGGAAAAGAAACTTCAAGCAAATTGGAAAAAGAAACTTGTAAGAAATCACACCCTATTCTATATGTGTCTTCTAAATCTACTCCAGAGACCCAGTGCCCTCAACAGTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFME
ADIITNLRCRLKEAEEERLKAAQYGLQLVESQNELQNQLDKCRNEMMTMT
ESYEQEKYTLQREVELKSRMLESLSCECEAIKQQQKMHLEKLEEQLSRSH
GQEVNELKTKIEKLKVELDEARLSEKQLKHQVDHQKELLSCKSEELRVMS
ERVQESMSSEMLALQIELTEMESMKTTLKEEVNELQYRQEQLELLITNLM
RQVDRLKEEKEEREKEAVSYYNALEKARVANQDLQVQLDQALQQALDPNS
KGNSLFAEVEDRRAAMERQLISMKVKYQSLKKQNVFNREQMQRMKLQIAT
LLQMKGSQTEFEQQERLLAMLEQKNGEIKHLLGEIRNLEKFKNLYDSMES
KPSVDSGTLEDNTYYTDLLQMKLDNLNKEIESTKGELSIQRMKALFESQR
ALDIERKLFANERCLQLSESENMKLRAKLDELKLKYEPEETVEVPVLKKR
REVLPVDITTAKDACVNNSALGGEVYRLPPQKEETQSCPNSLEDNNLQLE
KSVSIHTPVVSLSPHKNLPVDMQLKKEKKCVKLIGVPADAEALSERSGNT
PNSPRLAAESKLQTEVKEGKETSSKLEKETCKKSHPILYVSSKSTPETQC
PQQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48743
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Contains known variations Y506H and L586S (benign according to ensemble) and a silent C>T nucleotide change at 816 bp.