cDNA Clone
SKP1/Dia2 (yeast)

Plasmid
pET28a 8HIS SKP1 Dia2 G199-end (yeast)
Parent Plasmid
pET28a original
Expressed
yeast Dia2 G199-S732(end)
Genbank
NP_014723.2
Tag
6His
Species
Yeast
Sequence of Insert
GGATCAATGGTGACTTCTAATGTTGTCCTAGTGAGTGGTGAGGGTGAACGATTCACCGTAGACAAGAAAATCGCGGAGAGATCCCTGCTATTGAAAAACTATCTGAACGATATGCATGACAGCAACCTACAAAACAACTCAGATTCAGAATCAGATTCAGACTCGGAAACGAACCACAAAAGCAAAGACAACAATAACGGTGACGACGACGATGAGGATGACGACGAGATTGTGATGCCCGTGCCCAATGTTCGGTCTTCCGTTTTGCAGAAGGTTATCGAATGGGCCGAGCACCACAGAGACTCTAACTTCCCTGACGAAGATGACGACGATTCCAGAAAGAGTGCTCCCGTGGACTCATGGGACAGAGAATTCCTGAAAGTGGACCAAGAGATGCTATACGAAATTATTCTGGCGGCAAACTACCTCAACATCAAGCCCTTGCTTGACGCTGGCTGCAAGGTGGTTGCTGAAATGATCAGGGGAAGGTCTCCCGAGGAAATTAGAAGAACGTTTAATATCGTCAACGACTTCACTCCAGAGGAGGAGGCTGCCATTAGGCGTGAAAATGAATGGGCTGAAGACCGTTAGGCGGCCGCGATGGGTAGTACCAAGAAAACTGATTTAGTTGGCAATTTGCCGATAGAAATACTACCTATCATATTCCAAAGGTTTACCACTAAGGAGCTTGTTACGTTGTCGCTAGTTTGTAACAAATGGAGGGACAAAATTTTGTATCATTTAGACTGCTTCCAAGAATTCAACTTGGCACCAATAAATTTTAAAAATTTTGTAAAGTTTATGGATTTTTTACAACAGAATTTCACCAGGACGTATAGAAAATATATTCTTTCACAAGTAAAAGTCAGTTCTAGAATCACTTCGGAAGAGTTACGAATAACTCAATTATTATTCAGTAAAATGCCGAAATGTATAAACATCGAACGGCTTATACTGTCAATGCCAACACTAACTACAACACAAATCTTTAAGTTAATGGTTAGAGGAGGCACAGATTTTTTTACAAGGCTGCTGGAATTATCTCTCATGATAACTTATAGGCCTGATAAACAACATGAATTGGAGATACTGCAGACTTGTCCTCTGTTGAAAAAAATAGAATTAATTTTTGTCAATTCACTGGTACCAATTTTCGACGGAAATAATAGCGTTGGGAGGGATGGAAGTTTCAATGTGATGGCCCGTCATACTAATATGCAGATATCCACTGCGGATAATGATGAACAGGGAATAGTAGAAGAAAAGGTGATTTATAGTGAGCTAGAGAAGATAACATTGATATGTGATAAGAAGAAAATTAAGAATTTTCCACTTTGTCGTGCTCTATTAAGAGGCCAGTTTCCTCTATTGCAGAAACTGACGATAACTGGTGTAACATTTCCTATGAATAATCAAGACATAATGAATTTTCAATGGCTATTGAACTTCCCAGACCTAAAAGAACTATGGATAGAAGATAACGACAACTGTGAACTCAGTAAGTTTTTGCAATTGTTAAAATTTTCGAATGTCTGGAAAAACTTGGAAAAGTTAACCTTTAGAGAGAACAAGCTATATCCAATTGTTAATTTGGATGAAGATCAGCCTGTTACGAATGACGACGAGGTGCCATCCATGCTATTTTACAAGGAAAATCTCCAAAACCTGGAAAAGTTGGATCTAATGGGGACATCGATAAGTGGTTCTGCGTTGACTAGATTATGCGAGCAGGAATACTTGGATGGTCGGAAATTAAGAAGTTTAAACATTGGGAATTGCCCCAACATCCAGTTCCCAAATAATCACGCTCATACAGCAAGGATGATACTGGATGTGAATGCAGTTTTGAAGAGACTCTCCAAGTTGGAAGAAATAAATTTATCGCATCTCAGCTCCTTGAATGATAGTACGATGAAGTCATTCATTATTAATGTACCATTTTTGGAAAATTTAAAGAGGCTTGACATTTCGCACAATTTTGAAATTACAGGAATATCAATATATGAATTTCTAAAAAAATTTCAGATGGATCATGACAACGAAGCGGGAGGTCAACCACTGGCATATTTAAATATTGACGGATGTTCCCAAGTGTCTCATATCACAGTTAATATGATCCGAGCGCAGAACTTAGTTACTCAAGTGGATTGTGTTTATGAAAGAGATGTATGGAGAAAATTTGGAATTAATTCATATTCATACTCATAGGCGGCCGC
Amino Acid Sequence
MGHHHHHHHHENLYFQGGSMVTSNVVLVSGEGERFTVDKKIAERSLLLKNYLNDMHDSNLQNNSDSESDSDSETNHKSKDNNNGDDDDEDDDEIVMPVPNVRSSVLQKVIEWAEHHRDSNFPDEDDDDSRKSAPVDSWDREFLKVDQEMLYEIILAANYLNIKPLLDAGCKVVAEMIRGRSPEEIRRTFNIVNDFTPEEEAAIRRENEWAEDRZAAAMGSTKKTDLVGNLPIEILPIIFQRFTTKELVTLSLVCNKWRDKILYHLDCFQEFNLAPINFKNFVKFMDFLQQNFTRTYRKYILSQVKVSSRITSEELRITQLLFSKMPKCINIERLILSMPTLTTTQIFKLMVRGGTDFFTRLLELSLMITYRPDKQHELEILQTCPLLKKIELIFVNSLVPIFDGNNSVGRDGSFNVMARHTNMQISTADNDEQGIVEEKVIYSELEKITLICDKKKIKNFPLCRALLRGQFPLLQKLTITGVTFPMNNQDIMNFQWLLNFPDLKELWIEDNDNCELSKFLQLLKFSNVWKNLEKLTFRENKLYPIVNLDEDQPVTNDDEVPSMLFYKENLQNLEKLDLMGTSISGSALTRLCEQEYLDGRKLRSLNIGNCPNIQFPNNHAHTARMILDVNAVLKRLSKLEEINLSHLSSLNDSTMKSFIINVPFLENLKRLDISHNFEITGISIYEFLKKFQMDHDNEAGGQPLAYLNIDGCSQVSHITVNMIRAQNLVTQVDCVYERDVWRKFGINSYSYS*
Vector Type
Bacterial
Antibiotic
Amp
DU Number
DU25971
Unit Source
Protein Phosphorylation and Ubiquitylation Unit
Location
PPU
MTA
Dundee
Price
£125.00
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