cDNA Clone DNA2 (YEAST)

• Plasmid: pRS413 DNA2 D120-S1522(end) D212A D213A L215A I218A (YEAST) 6MYC KITRP 3'UTR
• Parent Plasmid: pRS413
• Expressed: DNA2 D120-S1522(end) D212A D213A L215A I218A (YEAST) 6MYC KITRP 3'UTR
• Genbank: NP_012034.1
• Species: Yeast

Sequence of Insert

Full sequence of cassette
GAGCTCGACGGCCCCATGGAAGAAGTAATATGGAAATATTCACCTTTACAACGAGATATGTCCGACAAAACAACAAGCGCTGCCGAATACTCTGATGACTACGAAGATGTCCAAAATCCCTCTTCTACACCTATAGTCCCTAATCGACTGAAAACTGTTCTTAGTTTCACAAATATCCAAGTACCCAACGCTGACGTAAATCAACTCATTCAAGAAAATGGAAATGAGCAAGTGCGTCCTAAACCAGCAGAAATATCTACCAGGGAGTCTCTCCGTAATATAGCTGCTATTGCCGATGATGCAGAGGGCGATTTAACTATAAAACCGACGATAACGAAATTCAGCGATTTGCCATCTTCACCCATCAAGGCACCCAACGTTGAAAAAAAAGCAGAGGTGAATGCAGAAGAAGTAGATAAAATGGATTCAACAGGAGATAGCAACGATGGCGATGACTCTTTGATAGATATTTTAACTCAAAAGTATGTTGAGAAACGCAAGAGTGAGAGTCAGATAACAATTCAAGGCAACACCAATCAAAAAAGTGGAGCCCAGGAAAGTTGTGGGAAGAATGATAACACAAAATCGAGAGGAGAAATTGAAGATCATGAAAATGTAGACAATCAAGCCAAAACAGGCAATGCATTTTATGAGAATGAAGAAGACAGTAATTGCCAAAGAATTAAAAAAAATGAAAAAATAGAATACAATTCTTCAGACGAATTTTCAGACGATTCACTAATTGAACTATTGAACGAAACTCAAACACAAGTGGAACCTAACACTATTGAACAAGATTTAGACAAAGTGGAGAAAATGGTCTCTGACGATTTGAGAATTGCTACTGATTCCACTTTATCCGCATATGCTCTCAGAGCTAAGTCTGGTGCTCCAAGAGACGGCGTAGTACGATTAGTAATAGTAAGCTTACGTAGTGTTGAATTGCCAAAAATTGGAACGCAAAAAATACTAGAGTGTATAGACGGCAAAGGCGAACAAAGTTCTGTTGTCGTTCGACATCCTTGGGTATATTTGGAATTTGAGGTTGGGGATGTAATACATATTATAGAAGGGAAAAATATTGAAAACAAACGGCTTCTTTCTGACGACAAAAACCCTAAAACTCAACTGGCTAACGACAACCTGTTGGTGCTAAACCCTGATGTATTATTTTCGGCAACATCAGTCGGAAGTTCAGTAGGTTGTTTAAGACGTTCAATTCTGCAAATGCAATTTCAAGATCCCCGCGGAGAACCAAGCCTTGTCATGACTTTAGGCAATATCGTACACGAGTTATTGCAAGACTCAATCAAATACAAGCTCTCGCATAATAAAATTTCTATGGAAATCATAATACAAAAGCTGGATTCTTTGTTAGAAACTTATTCTTTTTCAATTATCATTTGTAATGAAGAAATCCAGTACGTTAAAGAATTAGTTATGAAAGAACATGCTGAAAACATTTTGTACTTCGTCAATAAATTTGTTTCGAAAAGTAACTATGGTTGCTACACATCAATTTCCGGAACAAGAAGAACACAGCCAATTTCTATATCCAATGTGATTGATATTGAAGAAAACATTTGGTCTCCAATATATGGTCTTAAAGGGTTCCTAGATGCAACTGTTGAAGCTAATGTTGAAAACAATAAAAAACACATCGTTCCTTTAGAAGTGAAAACTGGAAAATCCAGAAGCGTTTCATACGAGGTTCAAGGCCTTATTTATACACTTCTGTTAAATGATCGCTATGAGATACCGATAGAGTTTTTTCTTCTTTATTTTACGAGGGATAAAAATATGACAAAGTTCCCTTCTGTTCTGCACTCCATTAAACATATTCTCATGTCCCGAAATAGAATGAGTATGAATTTTAAGCATCAACTCCAAGAGGTTTTCGGACAGGCTCAATCAAGGTTTGAATTACCACCCTTACTGCGCGATTCATCTTGTGATTCATGTTTCATCAAAGAATCATGCATGGTGTTGAATAAGCTACTAGAGGACGGGACACCAGAAGAAAGTGGCCTGGTAGAAGGTGAGTTTGAAATTTTGACAAACCATTTGTCGCAAAATCTCGCCAACTACAAAGAGTTTTTTACAAAATATAATGATCTAATAACTAAGGAGGAGTCAAGTATAACATGTGTAAATAAAGAACTTTTCTTATTGGATGGTTCCACAAGAGAATCCAGAAGTGGAAGGTGCCTTTCTGGCTTGGTTGTGTCAGAGGTGGTTGAACATGAAAAAACAGAGGGAGCGTATATATATTGCTTCTCAAGACGTAGAAATGATAATAACTCGCAGTCGATGTTATCATCTCAAATTGCTGCTAATGACTTTGTTATTATTAGTGATGAGGAGGGTCATTTTTGCTTGTGCCAAGGTCGTGTACAATTTATAAATCCCGCTAAAATTGGTATCTCCGTAAAACGAAAGTTATTAAACAATAGGCTTCTAGATAAGGAAAAAGGAGTCACTACCATCCAAAGTGTGGTCGAATCAGAGCTAGAACAATCCAGTCTCATAGCTACCCAAAATTTGGTCACATATAGAATCGACAAAAATGATATCCAACAGAGTTTGTCTTTAGCGCGATTTAATCTTTTAAGCTTGTTTTTACCAGCTGTTTCACCTGGCGTGGATATTGTTGACGAAAGGAGTAAATTATGTCGGAAGACAAAGAGATCTGATGGAGGTAATGAAATACTGCGATCACTTTTAGTTGATAATAGAGCACCTAAGTTTAGAGATGCAAATGATGATCCCGTCATTCCGTATAAGCTTTCAAAGGATACAACATTGAACTTGAATCAAAAAGAAGCAATAGACAAAGTAATGAGGGCCGAAGATTATGCATTGATATTGGGAATGCCAGGGACTGGTAAGACTACTGTTATCGCAGAAATTATTAAAATTCTCGTTTCCGAGGGGAAACGCGTGCTTTTGACATCATACACACATTCAGCAGTGGATAATATTTTAATAAAATTGAGGAACACCAACATTAGCATAATGAGATTGGGAATGAAACATAAGGTCCATCCTGACACGCAAAAATATGTCCCGAATTACGCGTCAGTGAAAAGTTATAACGATTATTTGTCCAAAATTAACAGCACCTCTGTTGTTGCTACCACCTGCTTAGGAATTAACGATATACTATTTACACTAAATGAAAAGGATTTTGATTATGTGATTTTGGACGAGGCAAGTCAAATTTCAATGCCTGTCGCTTTGGGACCTCTGCGGTATGGAAATAGGTTTATTATGGTAGGAGATCATTACCAGCTACCGCCATTAGTTAAAAATGACGCAGCTCGATTAGGTGGGTTAGAAGAGTCACTGTTCAAGACTTTCTGTGAAAAGCATCCGGAAAGCGTTGCAGAACTGACTTTACAGTACCGTATGTGCGGCGATATTGTAACTTTGTCTAATTTCCTAATTTATGATAATAAATTAAAATGCGGGAATAACGAAGTTTTTGCTCAATCTTTAGAGTTACCAATGCCTGAGGCCTTGTCGCGCTATAGGAATGAATCGGCTAACTCTAAGCAGTGGCTTGAGGATATACTAGAGCCTACAAGAAAAGTGGTATTTTTAAATTACGATAACTGCCCGGATATTATCGAACAAAGCGAAAAGGATAACATTACGAATCACGGGGAGGCAGAATTGACTCTACAATGTGTGGAAGGAATGTTATTGAGCGGCGTACCATGCGAAGATATTGGTGTAATGACGTTATACAGAGCACAGTTAAGATTACTGAAGAAGATATTCAACAAAAATGTGTATGACGGGCTAGAGATCTTGACTGCTGATCAGTTTCAAGGTCGTGACAAAAAGTGCATTATTATTTCCATGGTTAGAAGAAATTCTCAATTAAATGGAGGAGCGCTGCTGAAAGAGCTAAGAAGAGTCAATGTTGCCATGACAAGGGCCAAATCCAAGCTGATCATTATAGGTTCAAAAAGTACAATCGGCTCTGTTCCTGAAATAAAATCGTTCGTGAACCTGTTAGAAGAGCGTAATTGGGTCTATACAATGTGCAAGGATGCACTCTACAAATACAAATTTCCCGACAGATCAAACGCTATTGATGAGGCACGCAAAGGTTGTGGAAAAAGAACAGGTGCCAAACCCATCACATCAAAGTCTAAATTCGTAAGCGATAAACCTATCATAAAGGAAATTCTACAAGAGTATGAAAGTGAATTCCGTACGCTGCAGGTCGACTCCGGTTCTGCTGCTAGTGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGAAGAAGACTTGAACGGATCCTCTAGAGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGAAGAAGACTTGAACGGATCCACTAGCTAAAAAGTGGAACGATCATTCACTATATATATATCAATTTATATATACGTATGTGTAATTGAAGAAAGATACGTTTTTTCCTCTATTGAGAGGCCTGCTGGATGAATAGCTTTACCTTTTCTAAATCCTTGATACCATCAGTCTCTACTCCTCCACTTACATCGACACCGATCGCATTTGGTAACATATTAATGGCAACAGAAACGTTATCAGGATTCAATCCACCAGCGATAATGAATTTTATCTCGGGATGACTTGCAGACCAACTGGAAATTGCACTCCAATTCAATTTCTCACCAGTGCCACCTTCACCAGAATCGAACAACGTCAGCACATTGTCTACGTGTTCATACAGGTCCAGTAGTAATTCACAATCCTGTGGGAACTGGAACCTCTTAATGATTGGAATTGAAGATGGGATCAAAGATCTGTATTCTTTAATATCTTCATCTCCATGTAATTGTATCACATCTAGATTATATTCGTGGTACAGTTGAAGGACATCATCAACGGACTGATTTCTAAACACCCCGACTAGTTTAGTACCTTTCACGTTCTCTTGTTGGTGAACTGCAGTTGAAATACCTTTCGCAACAGATGAGTCAATGGTTCTTTTCCTACCGGGAACACAAATGATACCTAAGTAATCAGCACCATCATCCACAGCAGTCTTTGCAGCTTCAACGGTTTGCAAACCACACACTTTAACGAGCATCACAGTAGATTTCGCAAGAGGGTAGCTCGCTCAGAGTACCCAAGTAAATGATTAGTAAACTGATGTTTGATAGTTCAATTTTTCAATGAAATAACCTTATATTAAAATTGATATTACTATTATACAAAAATAAAGAATAAAGGATTTGAGTTTATACATAAAATACCATTATTATTTGTTCAGTGAGAGATACCGGGGTATATGGGATGTGTGTAGTGATACCATGCAATCATGTATCAAACATGGAAGAAGTGAAGGGGGAGAAAGAAACTTGAAAAAAAGAAAACCATTTGAAAAGAGGAAAAGTATCTAATTAAGATCTTTCACCTCTTAGTCTTCTGGCCTCGAGGCGAGCTCGCATTCATCGATTGACAGGGGGAATAAGAAGAGCTTCTCCATAACAGGAAAGCTATCACAGCATAAAATATCTATGTCATTTTATAAAGTTTGGTATATATAACACGTAATATTAGCATCTGAGGTTCGAAAGCTCGCATGTGTGTCTTTTCTAAAAAGTTTTCGTTTGAACTTTTCCAAAAGCTTCCTCATTAACGAACCAGGATGTTTTTTCACCAAATAATTTGTTAACCAATGCGGTTGGCAATTGATCGTTTTTCAAGTCAAAGATCTCATGCATTATATTTTGTTTGGAACTGCCCTCAGCCACAAAACACAGGGCTTTGGCGGGGCCC

Amino Acid Sequence

DGPMEEVIWKYSPLQRDMSDKTTSAAEYSDDYEDVQNPSSTPIVPNRLKTVLSFTNIQVPNADVNQLIQENGNEQVRPKPAEISTRESLRNIAAIADDAEGDLTIKPTITKFSDLPSSPIKAPNVEKKAEVNAEEVDKMDSTGDSNDGDDSLIDILTQKYVEKRKSESQITIQGNTNQKSGAQESCGKNDNTKSRGEIEDHENVDNQAKTGNAFYENEEDSNCQRIKKNEKIEYNSSDEFSDDSLIELLNETQTQVEPNTIEQDLDKVEKMVSDDLRIATDSTLSAYALRAKSGAPRDGVVRLVIVSLRSVELPKIGTQKILECIDGKGEQSSVVVRHPWVYLEFEVGDVIHIIEGKNIENKRLLSDDKNPKTQLANDNLLVLNPDVLFSATSVGSSVGCLRRSILQMQFQDPRGEPSLVMTLGNIVHELLQDSIKYKLSHNKISMEIIIQKLDSLLETYSFSIIICNEEIQYVKELVMKEHAENILYFVNKFVSKSNYGCYTSISGTRRTQPISISNVIDIEENIWSPIYGLKGFLDATVEANVENNKKHIVPLEVKTGKSRSVSYEVQGLIYTLLLNDRYEIPIEFFLLYFTRDKNMTKFPSVLHSIKHILMSRNRMSMNFKHQLQEVFGQAQSRFELPPLLRDSSCDSCFIKESCMVLNKLLEDGTPEESGLVEGEFEILTNHLSQNLANYKEFFTKYNDLITKEESSITCVNKELFLLDGSTRESRSGRCLSGLVVSEVVEHEKTEGAYIYCFSRRRNDNNSQSMLSSQIAANDFVIISDEEGHFCLCQGRVQFINPAKIGISVKRKLLNNRLLDKEKGVTTIQSVVESELEQSSLIATQNLVTYRIDKNDIQQSLSLARFNLLSLFLPAVSPGVDIVDERSKLCRKTKRSDGGNEILRSLLVDNRAPKFRDANDDPVIPYKLSKDTTLNLNQKEAIDKVMRAEDYALILGMPGTGKTTVIAEIIKILVSEGKRVLLTSYTHSAVDNILIKLRNTNISIMRLGMKHKVHPDTQKYVPNYASVKSYNDYLSKINSTSVVATTCLGINDILFTLNEKDFDYVILDEASQISMPVALGPLRYGNRFIMVGDHYQLPPLVKNDAARLGGLEESLFKTFCEKHPESVAELTLQYRMCGDIVTLSNFLIYDNKLKCGNNEVFAQSLELPMPEALSRYRNESANSKQWLEDILEPTRKVVFLNYDNCPDIIEQSEKDNITNHGEAELTLQCVEGMLLSGVPCEDIGVMTLYRAQLRLLKKIFNKNVYDGLEILTADQFQGRDKKCIIISMVRRNSQLNGGALLKELRRVNVAMTRAKSKLIIIGSKSTIGSVPEIKSFVNLLEERNWVYTMCKDALYKYKFPDRSNAIDEARKGCGKRTGAKPITSKSKFVSDKPIIKEILQEYESEFRTLQVDSGSAASGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGSSRGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGSTS*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU26034
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: The full cassette of DNA2 120-end D212A D213A L215A I218A 6myc KITRP1 3'UTR is in the sequence of insert field.; Use as template Internal Sac1 Site needs to be mutated