cDNA Clone MCM7 Yeast

• Plasmid: pMM40 (pRS305 Gal1 promoter MCM7 K29A K62A Yeast)
• Parent Plasmid: pRS305
• Expressed: MCM7 K29A K62A Yeast
• Species: Yeast

Sequence of Insert

ACTAGTATGAGTGCGGCACTTCCATCAATTCAGCTTCCTGTCGACTATAATAATCTTTTTAATGAAATCACCGATTTCTTGGTGACTTTCGCGCAGGATACGTTGTCTTCCGATGCAACACGAAATGAGAACGAAGATGAAAATTTGGATGCTGAGAATATCGAACAACATCTATTGGAAAAGGGGCCCGCGTACATGGCGATGCTACAGAAAGTGGCTAATAGGGAACTGAATTCCGTGATTATTGATTTAGATGACATTCTACAGTATCAAAACGAAAAGTTTCTCCAGGGCACACAGGCAGACGACCTTGTGTCTGCCATTCAGCAAAACGCTAATCACTTCACTGAATTATTTTGCCGTGCCATTGACAACAACATGCCGCTACCAACAAAAGAAATTGACTACAAGGACGACGTTCTTGATGTTATTTTAAACCAAAGGAGACTGAGGAATGAGAGAATGCTCTCGGACAGGACCAATGAGATCCGAAGCGAAAACCTCATGGATACTACGATGGATCCACCCTCTTCCATGAATGATGCGTTAAGAGAAGTTGTCGAGGATGAAACTGAGTTATTTCCTCCTAATTTAACTAGACGCTATTTCCTTTATTTTAAGCCTCTATCGCAAAATTGTGCTCGTCGTTACAGGAAGAAAGCAATTAGTTCTAAACCATTATCTGTTAGACAGATTAAAGGTGATTTCCTAGGCCAATTGATTACCGTCAGAGGTATTATCACCAGAGTTTCTGATGTCAAACCAGCTGTGGAAGTTATCGCATATACCTGCGATCAATGTGGGTACGAAGTTTTCCAAGAGGTCAACTCTCGTACTTTTACCCCGTTGTCAGAATGTACTTCAGAAGAATGTTCCCAAAATCAAACAAAAGGTCAATTGTTTATGAGTACACGTGCTTCCAAATTTAGTGCCTTTCAGGAATGTAAAATTCAGGAGTTATCACAACAAGTGCCAGTGGGTCATATTCCTAGGTCGCTAAATATCCACGTTAACGGGACGCTGGTAAGATCTTTATCGCCTGGTGATATCGTCGATGTAACTGGTATATTCTTGCCAGCGCCCTACACCGGTTTCAAAGCTTTGAAAGCTGGCTTACTGACAGAAACTTATTTAGAGGCACAGTTTGTCCGTCAACACAAGAAGAAATTTGCGTCTTTCAGTCTGACTTCTGATGTAGAAGAGAGAGTTATGGAATTAATCACCTCTGGTGATGTATATAATAGGCTGGCAAAATCTATTGCACCGGAAATCTACGGTAACTTGGACGTTAAGAAAGCGTTGCTGCTATTACTTGTCGGAGGTGTTGATAAAAGGGTAGGTGACGGTATGAAAATCAGAGGTGATATCAATGTTTGTCTGATGGGTGATCCCGGTGTTGCCAAATCTCAACTGCTGAAGGCCATTTGCAAAATATCACCTCGAGGAGTGTATACCACTGGTAAGGGTTCCTCAGGCGTTGGTCTGACCGCTGCCGTCATGAAAGATCCTGTCACGGATGAAATGATTCTAGAAGGTGGTGCCTTAGTACTCGCTGATAACGGTATTTGTTGTATCGATGAATTTGATAAGATGGATGAAAGTGACAGAACGGCAATCCATGAAGTTATGGAACAACAAACAATTTCGATATCCAAGGCGGGTATCAATACAACTTTGAACGCCAGAACCTCAATCTTAGCGGCAGCAAATCCGTTGTATGGTAGATATAATCCTAGATTATCACCTCTGGACAATATAAATCTACCAGCTGCTTTACTATCCAGATTTGATATTCTCTTCTTAATGTTAGATATACCAAGTAGAGATGACGACGAAAAATTAGCCGAGCACGTTACATATGTGCATATGCATAATAAACAACCGGATTTGGACTTCACTCCAGTAGAACCCTCTAAAATGAGAGAGTACATTGCCTATGCTAAGACAAAGAGGCCAGTGATGAGTGAAGCTGTAAACGATTATGTGGTGCAAGCTTACATCAGATTAAGACAAGACTCCAAGAGAGAAATGGATTCCAAATTTTCCTTTGGTCAAGCCACTCCGAGAACTTTACTAGGTATTATAAGATTATCTCAAGCGCTAGCAAAGTTAAGGTTGGCTGATATGGTGGATATAGATGATGTGGAGGAAGCCTTGAGGTTGGTCCGAGTCTCCAAGGAATCATTGTATCAAGAAACCAACAAATCTAAAGAAGATGAAAGCCCCACAACGAAAATCTTTACGATCATCAAGAAAATGTTACAAGAAACTGGTAAAAACACGCTATCATATGAAAATATTGTGAAAACTGTAAGACTAAGAGGGTTTACCATGCTACAATTAAGCAACTGTATCCAAGAATATTCTTACTTAAACGTCTGGCATTTAATTAATGAGGGTAACACTTTGAAATTCGTGGATGACGGTACCATGGACACAGACCAGGAAGACTCACTAGTGAGCACGCCTAAACTCGCACCACAAACGACCGCTTCCGCTAACGCGAGCGCCCAAGATTCTGATATCGATCTACAAGACGCTTGACCCGGG

Amino Acid Sequence

MSAALPSIQLPVDYNNLFNEITDFLVTFAQDTLSSDATRNENEDENLDAENIEQHLLEKGPAYMAMLQKVANRELNSVIIDLDDILQYQNEKFLQGTQADDLVSAIQQNANHFTELFCRAIDNNMPLPTKEIDYKDDVLDVILNQRRLRNERMLSDRTNEIRSENLMDTTMDPPSSMNDALREVVEDETELFPPNLTRRYFLYFKPLSQNCARRYRKKAISSKPLSVRQIKGDFLGQLITVRGIITRVSDVKPAVEVIAYTCDQCGYEVFQEVNSRTFTPLSECTSEECSQNQTKGQLFMSTRASKFSAFQECKIQELSQQVPVGHIPRSLNIHVNGTLVRSLSPGDIVDVTGIFLPAPYTGFKALKAGLLTETYLEAQFVRQHKKKFASFSLTSDVEERVMELITSGDVYNRLAKSIAPEIYGNLDVKKALLLLLVGGVDKRVGDGMKIRGDINVCLMGDPGVAKSQLLKAICKISPRGVYTTGKGSSGVGLTAAVMKDPVTDEMILEGGALVLADNGICCIDEFDKMDESDRTAIHEVMEQQTISISKAGINTTLNARTSILAAANPLYGRYNPRLSPLDNINLPAALLSRFDILFLMLDIPSRDDDEKLAEHVTYVHMHNKQPDLDFTPVEPSKMREYIAYAKTKRPVMSEAVNDYVVQAYIRLRQDSKREMDSKFSFGQATPRTLLGIIRLSQALAKLRLADMVDIDDVEEALRLVRVSKESLYQETNKSKEDESPTTKIFTIIKKMLQETGKNTLSYENIVKTVRLRGFTMLQLSNCIQEYSYLNVWHLINEGNTLKFVDDGTMDTDQEDSLVSTPKLAPQTTASANASAQDSDIDLQDA*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU26066
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee