cDNA Clone USP9X

• Synonyms: DFFRX, FAF, FAM
• Plasmid: pcDNA5D FRT/TO BirA R118G-USP9x (E2347G)-HA
• Parent Plasmid: pcDNA5D FRT/TO BirA R118G MCS HA
• Expressed: BirA R118G-USP9x (E2347G)-HA
• Genbank: Gene 8239 NM_001039591.2 GI:145309310 with silent changes: t856c, g6150a and a7062g. And coding changes a4904g (D1635G) and a7040g (E2347G); EST-derived.
• Tag: HA
• Tag: BirA
• Species: Human

Sequence of Insert

ggatccATGACAGCCACGACTCGTGGCTCTCCGGTCGGAGGGAATGACAA
CCAGGGCCAGGCTCCTGATGGACAGTCTCAGCCCCCCCTCCAACAGAATC
AGACTTCATCGCCTGATTCTTCCAATGAAAATTCCCCGGCAACTCCCCCA
GATGAGCAAGGTCAAGGTGATGCCCCACCACAGCTTGAAGATGAGGAACC
TGCATTTCCACATACTGACTTGGCCAAGTTGGATGACATGATCAACAGGC
CTCGATGGGTGGTTCCAGTTTTGCCGAAAGGGGAATTAGAAGTGCTTTTA
GAAGCTGCTATTGATCTTAGTAAAAAGGGCCTTGATGTTAAAAGTGAAGC
ATGTCAGCGATTTTTCCGTGATGGGCTAACAATATCATTCACTAAAATTC
TTACAGATGAAGCAGTGAGTGGCTGGAAGTTTGAAATTCATAGGTGTATT
ATTAACAATACTCATCGTCTGGTGGAGCTATGTGTGGCCAAGTTGTCCCA
AGACTGGTTTCCACTTTTAGAACTTCTTGCCATGGCCTTAAATCCTCATT
GCAAATTCCATATCTACAATGGTACACGTCCATGTGAATCAGTTTCCTCA
AGTGTTCAGTTGCCTGAAGATGAACTCTTTGCTCGTTCTCCAGATCCTCG
ATCACCAAAGGGTTGGCTAGTGGATCTTCTCAACAAATTTGGCACTTTAA
ATGGGTTCCAGATTTTGCATGATCGTTTTATTAATGGATCAGCATTAAAC
GTTCAAATAATTGCAGCCCTTATTAAACCATTTGGGCAATGCTATGAGTT
TCTCACTCTTCATACAGTGAAAAAGTACTTTCTTCCAATAATAGAAATGG
TTCCACAGTTTCTAGAAAACTTAACTGATGAAGAACTGAAAAAAGAAGCA
AAGAATGAAGCCAAAAATGATGCTCTTTCAATGATTATTAAATCTTTGAA
GAATTTAGCTTCAAGGGTTCCAGGACAAGAAGAAACTGTTAAAAACTTAG
AAATATTTAGGTTAAAAATGATACTTAGATTATTGCAAATTTCTTCTTTC
AATGGAAAGATGAATGCACTGAATGAAGTTAATAAGGTGATATCTAGTGT
ATCATACTATACTCATCGACATGGTAATCCTGAGGAGGAAGAGTGGCTCA
CAGCTGAACGAATGGCAGAATGGATACAGCAGAACAATATCTTATCCATA
GTGTTGCGAGATAGTCTTCATCAGCCACAGTATGTAGAAAAGTTAGAGAA
GATTCTTCGTTTTGTCATCAAAGAAAAAGCTCTGACCTTACAGGATCTTG
ATAATATCTGGGCAGCACAGGCAGGGAAACATGAAGCCATTGTGAAGAAT
GTACATGATCTCCTGGCAAAATTGGCATGGGATTTTTCTCCTGAACAACT
TGATCATCTTTTTGATTGTTTTAAGGCCAGTTGGACAAATGCGAGTAAAA
AGCAACGTGAAAAGCTACTTGAGCTGATACGTCGTCTTGCAGAAGATGAT
AAAGATGGTGTGATGGCACACAAAGTGTTGAACCTTCTGTGGAATCTGGC
TCACAGTGATGATGTGCCTGTAGATATCATGGACCTGGCTCTCAGTGCCC
ACATAAAAATACTAGATTACAGTTGCTCCCAGGACCGTGATACACAAAAG
ATCCAATGGATAGATCGCTTTATAGAAGAACTTCGCACAAATGACAAATG
GGTTATTCCCGCACTGAAACAAATTAGAGAAATTTGTAGTTTGTTTGGTG
AAGCGCCTCAAAATTTGAGTCAAACTCAGCGAAGTCCCCATGTGTTTTAT
CGCCATGACTTAATCAATCAACTTCAACACAATCATGCCCTAGTTACTTT
GGTAGCAGAAAACCTTGCAACTTACATGGAAAGCATGAGACTATATGCTA
GAGACCATGAAGATTATGACCCACAAACTGTGAGGCTGGGAAGTAGATAT
AGTCATGTTCAAGAAGTTCAAGAACGGCTTAACTTCCTTAGATTTTTATT
GAAGGATGGTCAGCTGTGGCTATGTGCTCCTCAGGCAAAACAAATATGGA
AATGCTTAGCTGAGAATGCAGTTTACCTTTGTGATCGTGAAGCCTGTTTT
AAGTGGTATTCCAAGTTGATGGGGGATGAACCAGACTTAGATCCTGATAT
TAATAAGGACTTCTTTGAAAGTAATGTGCTTCAGCTTGATCCTTCTCTGT
TAACTGAAAATGGAATGAAGTGTTTTGAGCGATTCTTCAAAGCTGTGAAT
TGTCGAGAAGGAAAACTAGTAGCAAAAAGGAGAGCCTATATGATGGATGA
CTTGGAGTTAATAGGATTAGATTACCTTTGGAGGGTCGTGATTCAGAGTA
ATGATGATATTGCCAGCAGAGCTATAGATCTCCTCAAAGAGATATACACG
AACCTTGGTCCAAGACTACAAGTCAATCAGGTGGTGATCCATGAAGACTT
CATTCAGTCTTGTTTTGATCGTCTGAAGGCTTCCTATGACACATTGTGTG
TTTTGGATGGTGACAAAGACAGTGTTAATTGTGCAAGACAGGAAGCTGTT
CGAATGGTTCGAGTATTAACTGTTTTAAGGGAATATATAAATGAATGTGA
CAGTGATTATCATGAGGAAAGAACAATTCTCCCTATGTCGAGAGCATTCC
GCGGTAAACACCTCTCTTTTGTAGTTCGATTTCCAAACCAGGGCAGACAG
GTTGATGACTTGGAGGTATGGTCTCATACAAATGATACAATTGGTTCAGT
ACGACGATGTATTCTCAATCGTATTAAAGCCAACGTAGCCCATACAAAAA
TTGAGCTCTTTGTGGGCGGTGAGCTGATAGATCCTGCAGATGATAGAAAG
TTGATTGGACAATTAAACTTAAAAGATAAATCGCTTATTACAGCCAAACT
TACACAGATAAGTTCCAATATGCCTTCAAGCCCTGATAGCTCTTCTGATT
CCTCCACTGGATCTCCTGGAAACCATGGTAATCATTACAGTGATGGTCCC
AATCCAGAAGTGGAAAGCTGTTTGCCTGGAGTGATAATGTCACTGCATCC
CAGATACATCTCTTTTCTTTGGCAAGTTGCAGACTTAGGTAGCAGCCTAA
ATATGCCACCCCTTAGAGATGGAGCAAGAGTACTTATGAAACTTATGCCG
CCAGATAGCACAACGATAGAAAAATTAAGAGCTATTTGTTTAGACCATGC
CAAACTTGGAGAAAGCAGCCTTAGTCCATCTCTTGACTCACTTTTCTTTG
GTCCTTCAGCCTCACAAGTGCTATATCTAACAGAGGTAGTCTATGCCTTG
TTAATGCCTGCTGGTGCACCTCTGGCTGATGATTCCTCTGATTTTCAGTT
TCACTTCTTGAAAAGTGGTGGCCTACCCCTTGTACTGAGTATGCTAACCA
GAAATAACTTCCTACCGAATGCAGATATGGAAACTCGAAGGGGTGCCTAC
CTCAATGCTCTTAAAATAGCCAAGCTTTTGCTAACTGCCATTGGCTATGG
TCATGTTCGAGCTGTGGCAGAAGCTTGTCAGCCAGGTGTAGAAGGTGTGA
ATCCCATGACACAGATCAACCAAGTTACCCATGATCAAGCAGTGGTGCTA
CAAAGTGCCCTTCAGAGCATTCCTAATCCATCATCCGAGTGCATGCTTAG
AAATGTGTCAGTTCGTCTTGCTCAGCAGATATCTGATGAGGCTTCAAGAT
ATATGCCTGATATTTGTGTAATTAGAGCTATACAAAAAATTATCTGGGCA
TCAGGATGTGGGTCGTTACAGCTAGTATTTAGCCCAAATGAAGAAATCAC
TAAAATTTATGAGAAGACCAATGCAGGCAATGAGCCAGACTTGGAAGACG
AACAGGTTTGCTGTGAAGCATTGGAAGTGATGACCTTATGTTTTGCCTTG
ATTCCAACAGCCTTAGATGCTCTTAGTAAAGAAAAGGCTTGGCAGACATT
CATCATTGACTTACTATTGCACTGTCACAGCAAAACTGTTCGTCAGGTGG
CACAGGAGCAGTTCTTTTTAATGTGCACCAGATGTTGCATGGGACACCGG
CCTCTACTTTTCTTCATTACTCTACTCTTTACTGTTTTGGGGAGCACAGC
AAGAGAGAGAGCTAAACACTCAGGCGACTACTTTACTCTTTTAAGACACC
TTCTTAATTACGCTTACAATAGTAATATTAATGTACCCAATGCTGAAGTT
CTTCTCAATAATGAAATTGATTGGCTTAAAAGAATTAGGGATGATGTTAA
AAGAACAGGAGAAACGGGTATTGAAGAGACGATCTTAGAGGGCCACCTTG
GAGTGACAAAGGAGTTACTGGCCTTTCAAACTTCTGAGAAAAAATTTCAT
ATTGGTTGTGAAAAAGGAGGTGCTAATCTCATTAAAGAATTAATTGATGA
TTTCATATTTCCTGCATCCAATGTTTACCTACAGTATATGAGAAATGGAG
AGCTTCCAGCTGAACAGGCTATTCCGGTCTGTGGTTCACCACCTACAATT
AATGCTGGTTTTGAATTACTTGTAGCATTAGCTGTTGGCTGTGTGAGGAA
TCTCAAACAAATAGTAGATTCTTTGACTGAAATGTATTACATTGGCACAG
CAATAACTACTTGTGAAGCACTTACTGAGTGGGAATATCTGCCACCTGTT
GGACCCCGCCCACCCAAAGGATTCGTGGGGCTGAAAAATGCCGGTGCTAC
TTGTTACATGAATTCTGTGATTCAGCAACTCTACATGATTCCTTCCATTA
GGAACGGTATTCTTGCCATTGAAGGCACAGGTAGTGATGTAGATGATGAT
ATGTCTGGGGATGAGAAGCAGGACAATGAGAGCAATGTTGATCCCAGGGA
TGATGTATTTGGATATCCTCAACAATTTGAAGATAAACCAGCATTAAGTA
AAACTGAAGATAGAAAAGAGTACAACATTGGTGTCCTAAGACACCTTCAG
GTCATCTTTGGTCATTTAGCTGCTTCTCGACTGCAATACTATGTGCCCAG
AGGATTTTGGAAACAGTTCAGGCTTTGGGGTGAGCCTGTTAATCTGCGTG
AACAACACGATGCTTTAGAATTTTTTAATTCATTGGTGGATAGTTTAGAT
GAAGCTTTAAAAGCTTTAGGACATCCAGCTATGCTAAGTAAAGTCTTAGG
AGGTTCCTTTGCTGATCAGAAGATCTGCCAAGGCTGCCCACATAGGTACG
AATGTGAAGAATCTTTTACGACCCTAAACGTAGACATTAGAAATCACCAA
AATCTTCTTGATTCTTTGGAACAGTATGTCAAAGGAGATTTACTAGAAGG
TGCAAATGCATATCATTGTGAAAAATGCAATAAAAAGGTTGATACCGTAA
AGCGCTTGCTGATTAAAAAATTACCTCCTGTTCTTGCTATACAACTAAAG
CGATTTGACTATGACTGGGAAAGAGAATGTGCAATCAAGTTCAATGATTA
TTTTGAATTTCCTCGAGAGCTGGACATGGAACCTTACACAGTTGCAGGTG
TCGCAAAGCTGGAAGGGGATAATGTAAACCCAGAGAGTCAGTTGATACAA
CAGAGTGAGCAGTCTGAAAGTGAGACAGCAGGAAGCACAAAATACAGACT
TGTGGGTGTGCTCGTACACAGTGGTCAAGCGAGTGGGGGGCATTATTATT
CTTACATCATCCAAAGGAATGGTGGAGATGGTGAGAGAAATCGCTGGTAT
AAATTTGATGATGGTGATGTAACAGAATGTAAAATGGATGATGACGAAGA
AATGAAAAACCAGTGTTTTGGTGGAGAGTACATGGGAGAAGTGTTTGATC
ACATGATGAAGCGTATGTCATACAGGCGCCAGAAAAGGTGGTGGAATGCT
TATATACTTTTTTATGAACGAATGGACACAATAGACCAAGATGATGAGTT
GATAAGATATATATCAGAGCTTGCTATCACCACCAGACCTCATCAGATTA
TTATGCCATCAGCCATTGAGAGAAGTGTACGGAAACAGAACGTACAATTC
ATGCATAACCGAATGCAGTACAGTATGGAGTATTTTCAGTTTATGAAAAA
ACTGCTTACATGTAATGGCGTTTACTTAAACCCTCCTCCCGGGCAAGATC
ACCTGTTGCCTGAAGCAGAAGAAATCACTATGATCAGTATTCAACTTGCT
GCTAGATTCCTCTTTACTACAGGATTTCACACAAAGAAAGTAGTCCGTGG
CTCTGCCAGTGATTGGTATGATGCATTGTGTATTCTCCTTCGTCACAGCA
AGAATGTACGTTTTTGGTTTGCTCATAACGTCCTTTTTAATGTTTCAAAT
CGCTTCTCCGAATACCTTCTGGAGTGCCCTAGTGCAGAAGTGAGGGGTGC
GTTTGCAAAACTTATAGTCTTTATTGCACATTTTTCCTTGCAAGATGGGC
CATGTCCTTCACCTTTTGCCTCTCCTGGACCTTCTAGTCAGGCTTATGAC
AACTTAAGCTTGAGTGATCACTTACTAAGAGCAGTACTAAATCTCTTGAG
AAGGGAAGTTTCAGAGCATGGGCGTCATTTACAGCAGTATTTCAACCTGT
TTGTAATGTATGCCAATTTAGGTGTGGCAGAGAAGACACAGCTTCTGAAA
TTGAGTGTACCTGCTACTTTTATGCTTGTGTCTTTAGATGAAGGTCCAGG
TCCTCCAATCAAATACCAGTATGCTGAATTAGGCAAATTATACTCAGTAG
TGTCACAGCTGATCCGCTGTTGCAATGTCTCTTCAAGAATGCAGTCTTCA
ATCAATGGTAATCCTCCTCTTCCCAATCCTTTTGGTGATCCTAATTTATC
ACAACCTATAATGCCAATTCAGCAGAATGTGGCAGACATTTTATTTGTGA
GAACAAGTTATGTGAAGAAAATCATTGAAGACTGCAGTAATTCAGAGGAA
ACCGTCAAATTGCTTCGTTTTTGCTGCTGGGAGAATCCTCAGTTCTCATC
TACTGTCCTCAGTGAACTTCTCTGGCAGGTTGCATATTCCTATACCTATG
AACTGCGGCCCTATTTGGATCTGCTTTTGCAAATCTTACTGATTGGGGAC
TCCTGGCAAACTCACAGGATTCATAATGCACTGAAAGGAATTCCAGATGA
CCGAGATGGGCTGTTTGACACAATCCAGCGCTCTAAGAATCACTATCAAA
AAAGAGCATACCAGTGTATAAAATGTATGGTAGCTCTATTTAGTAACTGT
CCTGTTGCTTACCAAATCCTGCAGGGCAATGGAGATCTTAAAAGAAAGTG
GACCTGGGCAGTGGAATGGCTTGGAGATGAACTTGAAAGAAGACCATATA
CTGGCAATCCTCAGTACACTTACAACAATTGGTCTCCCCCAGTGCAAAGC
AATGAAACGTCCAATGGTTATTTCTTGGAGAGATCACATAGTGCTAGGAT
GACACTTGCAAAAGCTTGTGAACTCTGTCCAGAGGAGGAGCCAGATGACC
AAGATGCTCCAGATGAACATGAGTCGCCTCCACCTGAAGATGCCCCATTG
TACCCCCATTCACCTGGATCTCAGTATCAACAGAATAACCATGTGCATGG
ACAGCCATATACAGGCCCAGCAGCACATCACATGAACAACCCTCAGAGAA
CTGGCCAACGAGCACAAGAAAATTATGAAGGCAGTGAAGAAGTATCCCCA
CCTCAAACCAAGGATCAAgcggccgc

Amino Acid Sequence

MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDV
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSMTATTRGSPVGGNDNQGQAPDGQSQPP
LQQNQTSSPDSSNENSPATPPDEQGQGDAPPQLEDEEPAFPHTDLAKLDD
MINRPRWVVPVLPKGELEVLLEAAIDLSKKGLDVKSEACQRFFRDGLTIS
FTKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLSQDWFPLLELLAMA
LNPHCKFHIYNGTRPCESVSSSVQLPEDELFARSPDPRSPKGWLVDLLNK
FGTLNGFQILHDRFINGSALNVQIIAALIKPFGQCYEFLTLHTVKKYFLP
IIEMVPQFLENLTDEELKKEAKNEAKNDALSMIIKSLKNLASRVPGQEET
VKNLEIFRLKMILRLLQISSFNGKMNALNEVNKVISSVSYYTHRHGNPEE
EEWLTAERMAEWIQQNNILSIVLRDSLHQPQYVEKLEKILRFVIKEKALT
LQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWT
NASKKQREKLLELIRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDL
ALSAHIKILDYSCSQDRDTQKIQWIDRFIEELRTNDKWVIPALKQIREIC
SLFGEAPQNLSQTQRSPHVFYRHDLINQLQHNHALVTLVAENLATYMESM
RLYARDHEDYDPQTVRLGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQA
KQIWKCLAENAVYLCDREACFKWYSKLMGDEPDLDPDINKDFFESNVLQL
DPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIGLDYLWRV
VIQSNDDIASRAIDLLKEIYTNLGPRLQVNQVVIHEDFIQSCFDRLKASY
DTLCVLDGDKDSVNCARQEAVRMVRVLTVLREYINECDSDYHEERTILPM
SRAFRGKHLSFVVRFPNQGRQVDDLEVWSHTNDTIGSVRRCILNRIKANV
AHTKIELFVGGELIDPADDRKLIGQLNLKDKSLITAKLTQISSNMPSSPD
SSSDSSTGSPGNHGNHYSDGPNPEVESCLPGVIMSLHPRYISFLWQVADL
GSSLNMPPLRDGARVLMKLMPPDSTTIEKLRAICLDHAKLGESSLSPSLD
SLFFGPSASQVLYLTEVVYALLMPAGAPLADDSSDFQFHFLKSGGLPLVL
SMLTRNNFLPNADMETRRGAYLNALKIAKLLLTAIGYGHVRAVAEACQPG
VEGVNPMTQINQVTHDQAVVLQSALQSIPNPSSECMLRNVSVRLAQQISD
EASRYMPDICVIRAIQKIIWASGCGSLQLVFSPNEEITKIYEKTNAGNEP
DLEDEQVCCEALEVMTLCFALIPTALDALSKEKAWQTFIIDLLLHCHSKT
VRQVAQEQFFLMCTRCCMGHRPLLFFITLLFTVLGSTARERAKHSGDYFT
LLRHLLNYAYNSNINVPNAEVLLNNEIDWLKRIRDDVKRTGETGIEETIL
EGHLGVTKELLAFQTSEKKFHIGCEKGGANLIKELIDDFIFPASNVYLQY
MRNGELPAEQAIPVCGSPPTINAGFELLVALAVGCVRNLKQIVDSLTEMY
YIGTAITTCEALTEWEYLPPVGPRPPKGFVGLKNAGATCYMNSVIQQLYM
IPSIRNGILAIEGTGSDVDDDMSGDEKQDNESNVDPRDDVFGYPQQFEDK
PALSKTEDRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEP
VNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGC
PHRYECEESFTTLNVDIRNHQNLLDSLEQYVKGDLLEGANAYHCEKCNKK
VDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPRELDMEPY
TVAGVAKLEGDNVNPESQLIQQSEQSESETAGSTKYRLVGVLVHSGQASG
GHYYSYIIQRNGGDGERNRWYKFDDGDVTECKMDDDEEMKNQCFGGEYMG
EVFDHMMKRMSYRRQKRWWNAYILFYERMDTIDQDDELIRYISELAITTR
PHQIIMPSAIERSVRKQNVQFMHNRMQYSMEYFQFMKKLLTCNGVYLNPP
PGQDHLLPEAEEITMISIQLAARFLFTTGFHTKKVVRGSASDWYDALCIL
LRHSKNVRFWFAHNVLFNVSNRFSEYLLECPSAEVRGAFAKLIVFIAHFS
LQDGPCPSPFASPGPSSQAYDNLSLSDHLLRAVLNLLRREVSEHGRHLQQ
YFNLFVMYANLGVAEKTQLLKLSVPATFMLVSLDEGPGPPIKYQYAELGK
LYSVVSQLIRCCNVSSRMQSSINGNPPLPNPFGDPNLSQPIMPIQQNVAD
ILFVRTSYVKKIIEDCSNSEETVKLLRFCCWENPQFSSTVLSELLWQVAY
SYTYELRPYLDLLLQILLIGDSWQTHRIHNALKGIPDDRDGLFDTIQRSK
NHYQKRAYQCIKCMVALFSNCPVAYQILQGNGDLKRKWTWAVEWLGDELE
RRPYTGNPQYTYNNWSPPVQSNETSNGYFLERSHSARMTLAKACELCPEE
EPDDQDAPDEHESPPPEDAPLYPHSPGSQYQQNNHVHGQPYTGPAAHHMN
NPQRTGQRAQENYEGSEEVSPPQTKDQAAAYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24756
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 49108 parental. Grow at 30 degrees. Compared to NCBI isoform 4 ref has silent changes: t856c, g6150a and a7062g and coding change at a7040g (E2347G); ; All USP9X plasmids MUST be grown at 30 degrees C or less to prevent recombination.