cDNA Clone WNK1

• Synonyms: HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1; PSK
• Plasmid: pTREG mod FLAG WNK1 D368A
• Parent Plasmid: pTREG Mod
• Expressed: FLAG-WNK1 D368A
• Genbank: NM_018979.3 GI:296939601 with changes - see notes
• Tag: FLAG
• Species: Human

Sequence of Insert

ATGGACTACAAGGACGACGATGACAAGTCTGGCGGCGCCGCAGAGAAGCAGAGCAGCACTCCCGGTTCCCTGTTCCTCTCGCCGCCGGCTCCTGCCCCCAAGAATGGCTCCAGCTCCGATTCCTCCGTGGGGGAGAAACTGGGAGCCGCGGCCGCCGACGCTGTGACCGGCAGGACCGAGGAGTACAGGCGCCGCCGCCACACTATGGACAAGGACAGCCGTGGGGCGGCCGCGACCACTACCACCACTGAGCACCGCTTCTTCCGCCGGAGCGTCATCTGCGACTCCAATGCCACTGCACTGGAGCTTCCCGGCCTTCCTCTTTCCCTGCCCCAGCCCAGCATCCCCGCGGCTGTCCCGCAGAGTGCTCCACCGGAGCCCCACCGGGAAGAGACCGTGACCGCCACCGCCACTTCCCAGGTAGCCCAGCAGCCTCCAGCCGCTGCCGCCCCTGGGGAACAGGCCGTCGCGGGCCCTGCCCCCTCGACTGTCCCCAGCAGTACCAGCAAAGACCGCCCAGTGTCCCAGCCTAGCCTTGTGGGGAGCAAAGAGGAGCCGCCGCCGGCGAGAAGTGGCAGCGGCGGCGGCAGCGCCAAGGAGCCACAGGAGGAACGGAGCCAGCAGCAGGATGATATCGAAGAGCTGGAGACCAAGGCCGTGGGAATGTCTAACGATGGCCGCTTTCTCAAGTTTGACATCGAAATCGGCAGAGGCTCCTTTAAGACGGTCTACAAAGGTCTGGACACTGAAACCACCGTGGAAGTCGCCTGGTGTGAACTGCAGGATCGAAAATTAACAAAGTCTGAGAGGCAGAGATTTAAAGAAGAAGCTGAAATGTTAAAAGGTCTTCAGCATCCCAATATTGTTAGATTTTATGATTCCTGGGAATCCACAGTAAAAGGAAAGAAGTGCATTGTTTTGGTGACTGAACTTATGACGTCTGGAACACTTAAAACGTATCTGAAAAGGTTTAAAGTGATGAAGATCAAAGTTCTAAGAAGCTGGTGCCGTCAGATCCTTAAAGGTCTTCAGTTTCTTCATACTCGAACTCCACCTATCATTCACCGCGATCTTAAATGTGACAACATCTTTATCACCGGCCCTACTGGCTCAGTCAAGATTGGAGCCCTCGGTCTGGCAACCCTGAAGCGGGCTTCTTTTGCCAAGAGTGTGATAGGTACCCCAGAGTTCATGGCCCCTGAGATGTATGAGGAGAAATATGATGAATCCGTTGACGTTTATGCTTTTGGGATGTGCATGCTTGAGATGGCTACATCTGAATATCCTTACTCGGAGTGCCAAAATGCTGCGCAGATCTACCGTCGCGTGACCAGTGGGGTGAAGCCAGCCAGTTTTGACAAAGTAGCAATTCCTGAAGTGAAGGAAATTATTGAAGGATGCATACGACAAAACAAAGATGAAAGATATTCCATCAAAGACCTTTTGAACCATGCCTTCTTCCAAGAGGAAACAGGAGTACGGGTAGAATTAGCAGAAGAAGATGATGGAGAAAAAATAGCCATAAAATTATGGCTACGTATTGAAGATATTAAGAAATTAAAGGGAAAATACAAAGATAATGAAGCTATTGAGTTTTCTTTTGATTTAGAGAGAGATGTCCCAGAAGATGTTGCACAAGAAATGGTAGAGTCTGGGTATGTCTGTGAAGGTGATCACAAGACCATGGCTAAAGCTATCAAAGACAGAGTATCATTAATTAAGAGGAAACGAGAGCAGCGGCAGTTGGTACGGGAGGAGCAAGAAAAAAAAAAGCAGGAAGAGAGCAGTCTCAAACAGCAGGTAGAACAATCCAGTGCTTCCCAGACAGGAATCAAGCAGCTCCCTTCTGCTAGCACCGGCATACCTACTGCTTCTACCACTTCAGCTTCAGTTTCTACACAAGTAGAACCTGAAGAACCTGAGGCAGATCAACATCAGCAACTACAGTACCAGCAACCCAGTATATCTGTGTTATCTGATGGGACGGTTGACAGTGGTCAGGGATCCTCTGTCTTCACAGAATCTCGAGTGAGCAGCCAACAGACAGTTTCATATGGTTCCCAACATGAACAGGCACATTCTACAGGCACAGTCCCAGGGCATATACCTTCTACTGTCCAAGCACAGTCTCAGCCCCATGGGGTATATCCACCCTCAAGTGTGGCACAGGGGCAGAGCCAGGGTCAGCCATCCTCAAGTAGCTTAACAGGGGTTTCATCTTCCCAACCCATACAACATCCTCAGCAGCAGCAGGGAATACAGCAGACAGCCCCTCCTCAACAGACAGTGCAGTATTCACTTTCACAGACATCAACCTCCAGTGAGGCCACTACTGCACAGCCAGTGAGTCAACCTCAAGCTCCACAAGTCTTGCCTCAAGTATCAGCTGGAAAACAGCTTCCAGTTTCCCAGCCAGTACCAACTATCCAAGGCGAACCTCAGATCCCAGTTGCGACACAACCCTCGGTTGTTCCAGTCCACTCTGGTGCTCATTTCCTTCCAGTGGGACAGCCGCTCCCTACTCCCTTGCTCCCTCAGTACCCTGTCTCTCAGATTCCCATATCAACTCCTCATGTGTCTACGGCTCAGACAGGTTTCTCATCCCTTCCCATCACAATGGCAGCTGGCATTACTCAGCCTCTGCTCACGTTGGCTTCATCTGCTACAACAGCTGCGATCCCGGGGGTATCAACTGTGGTTCCTAGTCAGCTTCCAACCCTTCTGCAGCCTGTGACTCAGCTGCCAAGTCAGGTTCACCCACAGCTCCTACAACCAGCAGTTCAGTCCATGGGAATACCAGCTAACCTTGGACAAGCTGCTGAGGTTCCACTTTCCTCTGGAGATGTTCTGTACCAGGGCTTCCCACCTCGACTGCCACCACAGTACCCAGGAGATTCAAATATTGCTCCCTCTTCCAACGTGGCTTCTGTTTGCATCCATTCTACAGTCCTATCCCCTCCCATGCCGACAGAAGTACTGGCTACACCTGGGTACTTTCCCACAGTGGTGCAGCCTTATGTGGAATCAAATCTTTTAGTTCCTATGGGTGGTGTAGGAGGACAGGTTCAAGTGTCCCAGCCAGGAGGGAGTTTAGCACAAGCCCCCACTACATCCTCCCAGCAAGCAGTTTTGGAGAGTACTCAGGGAGTCTCTCAGGTTGCTCCTGCAGAGCCAGTTGCAGTAGCACAGCCCCAAGCTACCCAGCCGACCACTTTGGCTTCCTCTGTAGACAGTGCACATTCAGATGTTGCTTCAGGTATGAGTGATGGCAATGAGAACGTCCCATCTTCCAGTGGAAGGCATGAAGGAAGAACTACAAAACGGCATTACCGAAAATCTGTAAGGAGTCGCTCTCGACATGAAAAAACTTCACGCCCAAAATTAAGAATTTTGAATGTTTCAAATAAAGGAGACCGAGTAGTAGAATGTCAATTAGAGACTCATAATAGGAAAATGGTTACATTCAAATTTGACCTAGATGGTGACAACCCCGAGGAGATAGCAACAATTATGGTGAACAATGACTTTATTCTAGCAATAGAGAGAGAGTCGTTTGTGGATCAAGTGCGAGAAATTATTGAAAAAGCTGATGAAATGCTCAGTGAGGATGTCAGTGTGGAACCAGAGGGTGATCAGGGATTGGAGAGTCTACAAGGAAAGGATGACTATGGCTTTTCAGGTTCTCAGAAGCTTGAAGGAGAGTTCAAACAACCAATTCCTGCGTCTTCCATGCCACAGCAAATAGGCATTCCTACCAGTTCTTTAACTCAAGTTGTTCATTCTGCGGGAAGGCGGTTTATAGTGAGTCCTGTGCCAGAAAGCCGATTACGAGAATCAAAAGTTTTCCCCAGTGAAATAACAGATACAGTTGCTGCCTCTACAGCTCAGAGCCCTGGAATGAACTTGTCTCACTCTGCATCATCCCTTAGTCTACAACAGGCCTTTTCTGAACTTAGACGTGCCCAAATGACAGAAGGACCCAACACAGCACCTCCAAACTTTAGTCATACAGGACCAACATTTCCAGTAGTACCTCCTTTCTTAAGTAGCATTGCTGGAGTCCCAACCACAGCAGCAGCCACAGCACCAGTCCCTGCAACAAGCAGCCCTCCTAATGACATTTCCACATCAGTAATTCAGTCTGAGGTTACAGTGCCCACTGAAGAGGGGATTGCTGGAGTTGCCACCAGCACAGGTGTGGTAACTTCGGGTGGTCTCCCCATACCACCTGTGTCTGAATCACCAGTACTTTCCAGCGTAGTTTCAAGTATCACAATACCTGCAGTTGTCTCAATATCTACTACATCCCCGTCACTTCAAGTCCCCACATCCACATCTGAGATCGTTGTTTCTAGTACAGCACTGTATCCTTCAGTAACAGTTTCAGCAACTTCAGCCTCTGCAGGGGGCAGTACTGCTACCCCAGGTCCTAAGCCTCCAGCTGTAGTATCTCAGCAGGCAGCAGGCAGCACTACTGTGGGAGCCACATTAACATCAGTTTCTACCACCACTTCATTCCCAAGCACAGCTTCACAGCTGTCCATTCAGCTTAGCAGCAGTACTTCTACTCCTACTTTAGCTGAAACCGTGGTAGTTAGCGCACACTCACTAGATAAGACATCTCATAGCAGTACAACTGGATTGGCTTTCTCCCTCTCTGCACCATCTTCCTCTTCCTCTCCTGGAGCAGGAGTGTCTAGTTATATTTCTCAGCCTGGTGGGCTGCATCCTTTGGTCATTCCATCAGTGATAGCTTCTACTCCTATTCTTCCCCAAGCAGCAGGACCTACTTCTACACCTTTATTACCCCAAGTACCTAGTATCCCACCCTTGGTACAGCCTGTTGCCAATGTGCCTGCTGTACAGCAGACACTAATTCATAGTCAGCCTCAACCAGCTTTGCTTCCCAACCAGCCCCATACTCATTGTCCTGAAGTAGATTCTGATACACAACCCAAAGCTCCTGGAATTGATGACATAAAGACTCTAGAAGAAAAGCTGCGGTCTCTGTTCAGTGAACACAGCTCATCTGGAGCTCAGCATGCCTCTGTCTCACTGGAGACCTCACTAGTCATAGAGAGCACTGTCACACCAGGCATCCCAACTACTGCTGTTGCACCAAGCAAACTCCTGACTTCTACCACAAGTACTTGCTTACCACCAACCAATTTACCACTAGGAACAGTTGCTTTGCCAGTTACACCAGTGGTCACACCTGGGCAAGTTTCTACCCCAGTCAGCACTACTACATCAGGAGTGAAACCTGGAACTGCTCCCTCCAAGCCACCTCTAACTAAGGCTCCGGTGCTGCCAGTGGGTACTGAACTTCCAGCAGGTACTCTACCCAGCGAGCAGCTGCCACCTTTTCCAGGACCTTCTCTAACCCAGTCCCAGCAACCTCTAGAGGATCTTGATGCTCAATTGAGAAGAACACTTAGTCCAGAGATTATCACAGTGACTTCTGCGGTTGGTCCTGTGTCCATGGCGGCTCCAACAGCAATCACAGAAGCAGGAACACAGCCTCAGAAGGGTGTTTCTCAAGTCAAAGAAGGCCCTGTCCTAGCGACTAGTTCAGGAGCTGGTGTTTTTAAGATGGGACGATTTCAGGTTTCTGTTGCAGCAGACGGTGCCCAGAAAGAGGGTAAAAATAAGTCAGAAGATGCAAAGTCTGTTCATTTTGAATCCAGCACCTCAGAGTCCTCAGTGCTATCAAGTAGTAGTCCAGAGAGTACCTTGGTGAAACCAGAGCCGAATGGCATAACCATCCCTGGTATCTCTTCAGATGTGCCAGAGAGTGCCCACAAAACTACTGCCTCAGAGGCAAAGTCAGACACTGGGCAGCCTACCAAGGTTGGACGTTTTCAGGTGACAACTACAGCAAACAAAGTGGGTCGTTTCTCTGTATCAAAAACTGAGGACAAGATCACTGACACAAAGAAAGAAGGACCAGTGGCATCTCCTCCTTTTATGGATTTGGAACAAGCTGTTCTTCCTGCTGTGATACCAAAGAAAGAGAAGCCTGAACTGTCAGAGCCTTCACATCTAAATGGGCCGTCTTCTGACCCGGAGGCCGCTTTTTTAAGTAGGGATGTGGATGATGGTTCCGGTAGTCCACACTCGCCCCATCAGCTGAGCTCAAAGAGCCTTCCTAGCCAGAATCTAAGTCAAAGCCTTAGTAATTCATTTAACTCCTCTTACATGAGTAGCGACAATGAGTCAGATATCGAAGATGAAGACTTAAAGTTAGAGCTGCGACGACTACGAGATAAACATCTCAAAGAGATTCAGGACCTGCAGAGTCGCCAGAAGCATGAAATTGAATCTTTGTATACCAAACTGGGCAAGGTGCCCCCTGCTGTTATTATTCCCCCAGCTGCTCCCCTTTCAGGGAGAAGACGACGACCCACTAAAAGCAAAGGCAGCAAATCTAGTCGAAGCAGTTCCTTGGGGAATAAAAGCCCCCAGCTTTCAGGTAACCTGTCTGGTCAGAGTGCAGCTTCAGTCTTGCACCCCCAGCAGACCCTCCACCCTCCTGGCAACATCCCAGAGTCCGGGCAGAATCAGCTGTTACAGCCCCTTAAGCCATCTCCCTCCAGTGACAACCTCTATTCAGCCTTCACCAGTGATGGTGCCATTTCAGTACCAAGCCTTTCTGCTCCAGGTCAAGGAACCAGCAGCACAAACACTGTTGGGGCAACAGTGAACAGCCAAGCCGCCCAAGCTCAGCCTCCTGCCATGACGTCCAGCAGGAAGGGCACATTCACAGATGACTTGCACAAGTTGGTAGACAATTGGGCCCGAGATGCCATGAATCTCTCAGGCAGGAGAGGAAGCAAAGGGCACATGAATTATGAGGGCCCTGGAATGGCAAGGAAGTTCTCTGCACCTGGGCAACTGTGCATCTCCATGACCTCGAACCTGGGTGGCTCTGCCCCCATCTCTGCAGCATCAGCTACCTCTCTAGGTCACTTCACCAAGTCTATGTGCCCCCCACAGCAGTATGGCTTTCCAGCTACCCCATTTGGCGCTCAATGGAGTGGGACGGGTGGCCCAGCACCACAGCCACTTGGCCAGTTCCAACCTGTGGGAACTGCCTCCTTGCAGAATTTCAACATCAGCAATTTGCAGAAATCCATCAGCAACCCCCCAGGCTCCAACCTGCGGACCACTTAA

Amino Acid Sequence

MDYKDDDDKSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAA
AADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATA
LELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAA
PGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKE
PQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTE
TTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVK
GKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRT
PPIIHRDLKCDNIFITGPTGSVKIGALGLATLKRASFAKSVIGTPEFMAP
EMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPA
SFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEE
DDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVE
SGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQ
VEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQY
QQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPG
HIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQPIQHPQQQ
QGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQL
PVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVS
QIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVV
PSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVL
YQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFP
TVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGV
SQVAPAEPVAVAQPQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGR
HEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNR
KMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEML
SEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGI
PTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGM
NLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSS
IAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGV
VTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVV
SSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSV
STTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAF
SLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTP
LLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDT
QPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGI
PTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTT
SGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQP
LEDLDAQLRRTLSPEIITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKE
GPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSES
SVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTK
VGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAV
IPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLP
SQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQS
RQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLG
NKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSD
NLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSR
KGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLC
ISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGG
PAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU48869
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: D368A. Drosophila expression construct.; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.; Silent changes compared with NM_018979.3 CDS:; ; t258c, a1287g, a1914g, g2328a, a3666g, t3667c, g3669t, a4185g, a5541g, c6828t.; ; Coding changes compared with NM_018979.3 CDS:; ; a3166c (T1056P); g4517c (C1506S); g5424t (M1808I); All three seen in other published sequences for WNK1