cDNA Clone IRGQ

• Plasmid: pBabeD Puro GFP IRGQ siRNA resistant
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP-IRGQ siRNA Resistant
• Genbank: NM_001007561.2 GI:209364510
• Tag: GFP
• Species: Human

Sequence of Insert

ATGCCTCCGCCGCAGGGTGACGTGACCGCCTTGTTCCTGGGGCCTCCGGGCTTGGGGAAGTCCGCGCTGATCGCAGCGCTGTGCGACAAGGATGTGGAGACGCTCGAGGCCCCCGAGGGACGGCCGGACTCCGGGGTTCCCAGCCTAAGAGCTGCGGGCCCAGGCCTTTTTCTGGGCGAGCTGAGCTGCCCACCCGCAGCGCCGGGGCCCTGGGCGGCGGAAGCCAACGTGCTGGTACTGGTGCTGCCCGGACCCGAGGGGAACGGGGAACCGTTGGCTCCAGCCCTGGGAGAGGCAGCGCTGGCCGCCCTGGCCCGAGGGACCCCGCTACTGGCTGTGCGGAACCTCCGTCCTGGGGATTCACAGACTGCCGCCCAGGCCCGTGATCAGACAGCAGCTCTGCTGAACAGCGCGGGGTTAGGAGCTGCGGATCTGTTTGTGCTACCGGCGAACTGCGGCAGCAGCGACGGCTGCGAGGAGCTAGAGCGCCTCCGGGCGGCGCTGCAGAGCCAGGCAGAAGCGCTGCGGAGGCTCCTGCCACCGGCGCAGGATGGCTTCGAGGTGTTGGGTGCAGCAGAGCTAGAGGCTGTGCGTGAGGCCTTTGAGACCGGCGGCCTTGAGGCTGCGCTGTCGTGGGTGCGCTCAGGCCTGGAGCGCCTGGGCAGCGCACGGCTAGACCTGGCCGTGGCTGGCAAGGCTGACGTGGGCCTTGTGGTGGACATGCTGCTTGGATTGGATCCTGGCGACCCAGGCGCTGCGCCTGCTTCGGTCCCCACAGCACCCACTCCCTTCCCAGCCCCAGAGCGCCCGAATGTGGTGCTCTGGACCGTGCCTCTGGGCCACACGGGCACTGCCACCACCGCGGCCGCCGCCTCTCACCCAACGCACTACGACGCCCTCATCCTCGTCACCCCTGGGGCCCCCACTGAGAAGGACTGGGCCCAGGTCCAGGCCTTGCTGCTACCAGATGCGCCTCTTGTCTGCGTGCGCACAGACGGCGAGGGCGAGGATCCGGAGTGTCTGGGAGAAGGCAAAATGGAAAACCCTAAGGGCGAGAGCTTAAAGAACGCAGGTGGAGGGGGATTGGAGAATGCACTCAGTAAGGGAAGGGAGAAATGTAGCGCTGGATCGCAGAAAGCAGGCAGCGGGGAAGGTCCTGGGAAAGCTGGCAGCGAGGGTTTGCAGCAGGTTGTCGGCATGAAGAAATCAGGTGGTGGCGACTCAGAGCGGGCCGCTGCGTTAAGCCCGGAGGACGAGACGTGGGAGGTGCTGGAGGAGGCGCCGCCGCCAGTGTTCCCCCTACGGCCTGGCGGACTCCCAGGGCTATGCGAATGGCTGCGGCGAGCGCTCCCCCCAGCCCAGGCAGGGGCACTGCTGCTGGCGTTGCCACCAGCATCTCCCAGCGCTGCCCGAACCAAGGCTGCGGCGTTGCGAGCCGGGGCGTGGAGGCCAGCTCTGCTGGCTAGTCTGGCGGCGGCGGCGGCACCACTCCCAGGGCTGGGCTGGGCATGCGACGTGGCACTTCTGCGGGGTCAGCTGGCGGAGTGGCGACGGGGCCTGGGGCTGGAACCCACGGCACTGGCTCGACGTGAGCGTGCCCTGGGCCTGGCTTCTGGAGAGCTGGCAGCGCGCGCTCATTTCCCAGGCCCGGTGACGCGCGCCGAGGTGGAAGCAAGACTGGGCGCCTGGGCGGGCGAGGGCACTGCTGGGGGCGCAGCACTGGGGGCTCTCTCCTTCCTGTGGCCTGCGGGTGGTGCAGCGGCGACAGGTGGCCTGGGCTACCGAGCGGCTCACGGCGTCCTGCTGCAGGCTCTCGATGAGATGCGGGCTGATGCTGAGGCTGTGCTGGCACCCCCTGAGCCTGCCCAGTGAGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMPPPQGDVTALFLGPPGLGKSALIAALCDKDVETLEAPEGRPDSGVPSLRAAGPGLFLGELSCPPAAPGPWAAEANVLVLVLPGPEGNGEPLAPALGEAALAALARGTPLLAVRNLRPGDSQTAAQARDQTAALLNSAGLGAADLFVLPANCGSSDGCEELERLRAALQSQAEALRRLLPPAQDGFEVLGAAELEAVREAFETGGLEAALSWVRSGLERLGSARLDLAVAGKADVGLVVDMLLGLDPGDPGAAPASVPTAPTPFPAPERPNVVLWTVPLGHTGTATTAAAASHPTHYDALILVTPGAPTEKDWAQVQALLLPDAPLVCVRTDGEGEDPECLGEGKMENPKGESLKNAGGGGLENALSKGREKCSAGSQKAGSGEGPGKAGSEGLQQVVGMKKSGGGDSERAAALSPEDETWEVLEEAPPPVFPLRPGGLPGLCEWLRRALPPAQAGALLLALPPASPSAARTKAAALRAGAWRPALLASLAAAAAPLPGLGWACDVALLRGQLAEWRRGLGLEPTALARRERALGLASGELAARAHFPGPVTRAEVEARLGAWAGEGTAGGAALGALSFLWPAGGAAATGGLGYRAAHGVLLQALDEMRADAEAVLAPPEPAQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU26071
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: nucleotide sequence nt1033-1047 AAG ATG GAG AAT CCC; mutated to AAA ATG GAA AAC CCT