Synonyms
NY-CO-43, P/OKcl.13, PRKWNK2, SDCCAG43
Plasmid
pX335 WNK2 D342A antisense.1
Parent Plasmid
pX335
Expressed
WNK2 D342A antisense.1 guide
Genbank
NP_005100
Species
Human
Vector Type
Other
Antibiotic
Amp
DU Number
DU48784
Unit Source
Location
PPU
MTA
Notes
CRISPR guide plasmid. Targets antisense strand in exon 3, designed to introduce a D342A point mutation when used in conjunction with sense guide 48775 and a ssODN donor (D10A approach). Guide sequence shown as sequence of insert and donor shown as expression cassette. Positives may be enriched for with puromycin prior to further selection using western blotting with a phospho specific antibody or RFLP using the SfoI site introduced upon recombination.
Price
£125.00
To submit an order for this reagent acceptance of the Dundee Material Transfer Agreement is required at checkout.