cDNA Clone STAT3 mouse

• Synonyms: 1110034C02Rik, AW109958, Aprf
• Plasmid: pX335 STAT3m KO antisense B
• Parent Plasmid: pX335
• Expressed: STAT3m KO antisense B guide
• Genbank: NM_213659.2
• Species: Mouse

Sequence of Insert

GGAAGCTGTCGCTGTACAGC

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU48863
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR guide plasmid. Targets antisense strand in exon 2, designed to KO STAT3m when co-transfected with sense guide 48846. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.