cDNA Clone SENP1

• Plasmid: pSc-B-(GGSG X2) SENP1 (aa415-602)-(GGSG)-eGFP-SV40-SENP1 (aa415-644)
• Parent Plasmid: pSC-B
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGGTGGTTCTGGTGGTGGTTCTGGTGATAGTGAAGATGAATTTCC
TGAAATTACAGAGGAAATGGAGAAAGAAATAAAGAATGTATTTCGTAATG
GGAATCAGGATGAAGTTCTCAGTGAAGCATTTCGCCTGACCATTACACGC
AAAGATATTCAAACTCTAAACCATCTGAATTGGCTCAATGATGAGATCAT
CAATTTCTACATGAATATGCTGATGGAGCGAAGTAAAGAGAAGGGCTTGC
CAAGTGTGCATGCATTTAATACCTTTTTCTTCACTAAATTAAAAACGGCT
GGTTATCAGGCAGTGAAACGTTGGACAAAGAAAGTAGATGTATTTTCTGT
TGACATTCTTTTGGTGCCCATTCACCTGGGAGTACACTGGTGTCTAGCTG
TTGTGGACTTTAGAAAGAAGAATATTACCTATTACGACTCCATGGGTGGG
ATAAACAATGAAGCCTGCAGAATACTCTTGCAATACCTAAAGCAAGAAAG
CATTGACAAGAAAAGGAAAGAGTTTGACACCAATGGCTGGCAGCTTTTCA
GCAAGAAAAGCCAGGAGATTCCTCAGCAGATGAATGGAAGTGACGGTGGT
TCTGGTATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCAT
CCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCG
GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATC
TGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCT
GACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGC
ACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACC
ATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTT
CGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCA
AGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGC
CACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAA
CTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACC
ACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGAC
AACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAA
GCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTC
TCGGCATGGACGAGCTGTACAAGTAAAGCGGCCGCGACTCTAGATCATAA
TCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCA
CACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAA
CTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAA
ATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCC
AAACTCATCAATGTATCTTAGATAGTGAAGATGAATTTCCTGAAATTACA
GAGGAAATGGAGAAAGAAATAAAGAATGTATTTCGTAATGGGAATCAGGA
TGAAGTTCTCAGTGAAGCATTTCGCCTGACCATTACACGCAAAGATATTC
AAACTCTAAACCATCTGAATTGGCTCAATGATGAGATCATCAATTTCTAC
ATGAATATGCTGATGGAGCGAAGTAAAGAGAAGGGCTTGCCAAGTGTGCA
TGCATTTAATACCTTTTTCTTCACTAAATTAAAAACGGCTGGTTATCAGG
CAGTGAAACGTTGGACAAAGAAAGTAGATGTATTTTCTGTTGACATTCTT
TTGGTGCCCATTCACCTGGGAGTACACTGGTGTCTAGCTGTTGTGGACTT
TAGAAAGAAGAATATTACCTATTACGACTCCATGGGTGGGATAAACAATG
AAGCCTGCAGAATACTCTTGCAATACCTAAAGCAAGAAAGCATTGACAAG
AAAAGGAAAGAGTTTGACACCAATGGCTGGCAGCTTTTCAGCAAGAAAAG
CCAGGAGATTCCTCAGCAGATGAATGGAAGTGACTGTGGGATGTTTGCCT
GCAAATATGCTGACTGTATTACCAAAGACAGACCAATCAACTTCACACAG
CAACACATGCCATACTTCCGGAAGCGGATGGTCTGGGAGATCCTCCACCG
AAAACTCTTGTAGGTCGAC

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU24771
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: cloned overlap PCR product - SENP1 fragment with 5' and 3' linkers fused to eGFP-SV40 sequence. WT SENP1 fragment inserted into HindIII-Sal1 sites and resulting 33bp of pSc-B between nt1570-1601 removed with NW3414/3415. Entire SENP-eGFP-SV40-SENP cassette can be excised with BamHI-Sal1.