cDNA Clone UBCH5b

• Plasmid: pET28a(+) UBE2D2 (UBCH5b) C21S
• Parent Plasmid: pET28a original
• Expressed: His-UBE2D2 (UBCH5b) C21S
• Genbank: NM_003339
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGCTCTGAAGAGAATCCACAAGGAATTGAATGATCTGGCACGGGACCCTCCAGCACAGTCTTCAGCAGGTCCTGTTGGAGATGATATGTTCCATTGGCAAGCTACAATAATGGGGCCAAATGACAGTCCCTATCAGGGTGGAGTATTTTTCTTGACAATTCATTTCCCAACAGATTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGTAATGGCAGCATTTGTCTTGATATTCTACGATCACAGTGGTCTCCAGCACTAACTATTTCAAAAGTACTCTTGTCCATCTGTTCTCTGTTGTGTGATCCCAATCCAGATGATCCTTTAGTGCCTGAGATTGCTCGGATCTACAAAACAGATAGAGAAAAGTACAACAGAATAGCTCGGGAATGGACTCAGAAGTATGCGATGTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSALKRIHKELNDLARDP
PAQSSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKV
AFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDD
PLVPEIARIYKTDREKYNRIAREWTQKYAM*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU24782
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Ala35 of fusion = Ala2 of NM_003339