cDNA Clone HARBI1

• Synonyms: C11orf77, bA379C10.2; C9orf90
• Plasmid: pcDNA3.1 puro npGFP HARBI1 TIR/HARBI1 D225A 2A RFP NAIF1
• Parent Plasmid: pcDNA3.1 puro
• Expressed: HARBI1 D225A 2A RFP NAIF1
• Genbank: NM_173811.3 GI:224549031 (HARBI1) NM_197956.3 GI:215422409 (NAIF1)
• Tag: dsRED
• Species: Human

Sequence of Insert

AAGCTTGCCACCATGGCTATACCAATAACAGTGCTTGACTGTGACCTCTTGCTATATGGCCGTGGTCACCGGACATTGGACCGTTTTAAGCTGGATGATGTGACTGATGAATACTTGATGTCCATGTATGGGTTTCCACGGCAGTTCATTTATTACTTGGTGGAGCTCTTGGGGGCGAATCTTTCTAGGCCTACTCAGCGATCCAGGGCTATTAGCCCAGAGACACAGGTCCTTGCAGCATTGGGTTTTTATACCTCAGGTTCCTTCCAGACTCGGATGGGAGATGCCATTGGAATCAGTCAGGCGTCTATGAGTCGTTGTGTTGCCAATGTCACTGAAGCACTTGTGGAAAGGGCCTCACAGTTCATTCGCTTTCCAGCTGATGAAGCCTCCATTCAGGCTCTGAAGGATGAATTCTATGGGTTGGCAGGGATGCCAGGGGTGATGGGGGTGGTTGACTGTATCCATGTGGCCATCAAGGCACCAAATGCTGAAGACCTCTCCTATGTGAACCGAAAAGGCCTGCATTCTTTAAACTGCCTGATGGTGTGTGACATTAGAGGGACACTAATGACCGTGGAGACAAACTGGCCCGGCAGCCTACAGGACTGTGCTGTGCTGCAGCAGTCTTCCCTCAGTAGTCAGTTTGAAGCGGGTATGCACAAAGATAGCTGGCTTCTGGGTGCCAGTTCCTTCTTTCTTCGAACCTGGCTCATGACCCCACTTCACATTCCTGAAACTCCAGCAGAATATCGCTATAACATGGCCCATTCTGCAACTCACAGTGTGATTGAGAAGACTTTCCGAACCCTCTGCTCCCGATTCCGCTGCCTGGATGGATCCAAGGGGGCACTGCAGTACTCACCAGAGAAATCCAGCCATATCATCTTGGCCTGTTGTGTCCTCCACAACATCTCCCTGGAGCATGGGATGGATGTTTGGTCCTCTCCAATGACAGGACCCATGGAACAGCCCCCGGAAGAGGAGTATGAGCACATGGAGTCCCTGGACTTAGAGGCTGACCGTATTCGTCAGGAGCTAATGCTCACTCATTTTAGCGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCAATGGACAACACCGAGGACGTCATCAAGGAGTTCATGCAGTTCAAGGTGCGCATGGAGGGCTCCGTGAACGGCCACTACTTCGAGATCGAGGGCGAGGGCGAGGGCAAGCCCTACGAGGGCACCCAGACCGCCAAGCTGCAGGTGACCAAGGGCGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCCCAGTTCCAGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACATGAAGCTGTCCTTCCCCGAGGGCTTCACCTGGGAGCGCTCCATGAACTTCGAGGACGGCGGCGTGGTGGAGGTGCAGCAGGACTCCTCCCTCCAGGACGGCACCTTCATCTACAAGGTGAAGTTCAAGGGCGTGAACTTCCCCGCCGACGGCCCCGTAATGCAGAAGAAGACTGCCGGCTGGGAGCCCTCCACCGAGAAGCTGTACCCCCAGGACGGCGTGCTGAAGGGCGAGATCTCCCACGCCCTGAAGCTGAAGGACGGCGGCCACTACACCTGCGACTTCAAGACCGTGTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCAACCACTACGTGGACTCCAAGCTGGACATCACCAACCACAACGAGGACTACACCGTGGTGGAGCAGTACGAGCACGCCGAGGCCCGCCACTCCGGCTCCCAGACCGGTATGGCCGTCCCAGCCAAGAAAAGGAAGATGAACTTCTCAGAGCGGGAGGTGGAGATCATCGTGGAGGAGCTGGAGCTGAAGAAGCACCTGCTGGTGAACCACTTCAACGCCGGGGTACCCCTGGCCGCCAAGAGTGCGGCCTGGCACGGCATCCTGAGAAGGGTCAACGCCGTGGCCACCTGCCGCAGAGAGCTGCCTGAGGTCAAGAAGAAGTGGTCTGACCTCAAGACCGAGGTCCGTCGCAAGGTTGCCCAGGTCCGGGCCGCCGTGGAGGGTGGTGAGGCGCCGGGGCCCACTGAGGAGGACGGAGCTGGGGGGCCTGGGACAGGCGGTGGCAGTGGCGGCGGTGGCCCAGCTGTAGCCCCAGTGCTGCTGACCCCCATGCAACAACGTATCTGCAACCTGCTGGGCGAGGCCACCATCATCAGCCTGCCCAGCACCACAGAGATCCACCCTGTGGCCCTCGGACCCTCGGCCACCGCAGCCGCAGCCACGGTCACCCTGACACAGATCCCCACAGAGACCACCTATCACACGCTGGAGGAGGGCGTGGTGGAGTACTGCACGGCTGAGGCGCCCCCACCTCTGCCACCAGAGACCCCTGTGGACATGATGGCCCAGCATGCAGACACGTCGGTCAAGCCGCAAGCGCTCAAGAGCCGCATTGCTCTCAACTCCGCCAAGCTGATACAGGAGCAGCGGGTCACCAACCTGCATGTGAAGGAGATCGCACAGCACCTGGAACAGCAGAACGACCTACTGCAGATGATCCGCCGCTCCCAGGAAGTGCAGGCCTGTGCCCAGGAGCGCCAGGCCCAGGCCATGGAGGGCACACAGGCTGCCCTGAGCGTCCTCATCCAGGTCCTCCGGCCTATGATCAAAGATTTCCGCCGCTACCTGCAGAGCAACACAGCTAACCCGGCCCCCGCCTCTGACCCTGGGCAGGTGGCCCAGAATGGGCAGCCAGACAGCATCATCCAGTGAGCGGCCGC

Amino Acid Sequence

MAIPITVLDCDLLLYGRGHRTLDRFKLDDVTDEYLMSMYGFPRQFIYYLVELLGANLSRPTQRSRAISPETQVLAALGFYTSGSFQTRMGDAIGISQASM
SRCVANVTEALVERASQFIRFPADEASIQALKDEFYGLAGMPGVMGVVDCIHVAIKAPNAEDLSYVNRKGLHSLNCLMVCDIRGTLMTVETNWPGSLQDC
AVLQQSSLSSQFEAGMHKDSWLLGASSFFLRTWLMTPLHIPETPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLDGSKGALQYSPEKSSHIILACCVLHN
ISLEHGMDVWSSPMTGPMEQPPEEEYEHMESLDLEADRIRQELMLTHFSEGRGSLLTCGDVEENPGPMDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEG
EGKPYEGTQTAKLQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGVVEVQQDSSLQDGTFIYKVKFKGVNFPADG
PVMQKKTAGWEPSTEKLYPQDGVLKGEISHALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHSGSQTGMAVPAK
KRKMNFSEREVEIIVEELELKKHLLVNHFNAGVPLAAKSAAWHGILRRVNAVATCRRELPEVKKKWSDLKTEVRRKVAQVRAAVEGGEAPGPTEEDGAGG
PGTGGGSGGGGPAVAPVLLTPMQQRICNLLGEATIISLPSTTEIHPVALGPSATAAAATVTLTQIPTETTYHTLEEGVVEYCTAEAPPPLPPETPVDMMA
QHADTSVKPQALKSRIALNSAKLIQEQRVTNLHVKEIAQHLEQQNDLLQMIRRSQEVQACAQERQAQAMEGTQAALSVLIQVLRPMIKDFRRYLQSNTAN
PAPASDPGQVAQNGQPDSIIQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU50300
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct contains GFP (lacking promoter) flanked with HARBI1 TIR (Terminal Inverted Repeat) sites cloned into MfeI(MunI) site of pcDNA3.1 puro, and HARBI1 D225A-2A RFP-NAIF1 cloned downstream of CMV promoter.