cDNA Clone DNA2 (YEAST)

• Plasmid: pRS413 DNA2 D120-S1522(end) D212A D213A L215A I218A (YEAST) 6MYC KITRP 3'UTR
• Parent Plasmid: pRS413
• Expressed: DNA2 D120-S1522(end) D212A D213A L215A I218A (YEAST) 6MYC KITRP 3'UTR
• Genbank: NP_012034.1
• Species: Yeast

Sequence of Insert

Full sequence of cassette
GAGCTCGACGGCCCCATGGAAGAAGTAATATGGAAATATTCACCTTTACAACGAGATATGTCCGACAAAACAACAAGCGCTGCCGAATACTCTGATGACTACGAAGATGTCCAAAATCCCTCTTCTACACCTATAGTCCCTAATCGACTGAAAACTGTTCTTAGTTTCACAAATATCCAAGTACCCAACGCTGACGTAAATCAACTCATTCAAGAAAATGGAAATGAGCAAGTGCGTCCTAAACCAGCAGAAATATCTACCAGGGAGTCTCTCCGTAATATAGCTGCTATTGCCGATGATGCAGAGGGCGATTTAACTATAAAACCGACGATAACGAAATTCAGCGATTTGCCATCTTCACCCATCAAGGCACCCAACGTTGAAAAAAAAGCAGAGGTGAATGCAGAAGAAGTAGATAAAATGGATTCAACAGGAGATAGCAACGATGGCGATGACTCTTTGATAGATATTTTAACTCAAAAGTATGTTGAGAAACGCAAGAGTGAGAGTCAGATAACAATTCAAGGCAACACCAATCAAAAAAGTGGAGCCCAGGAAAGTTGTGGGAAGAATGATAACACAAAATCGAGAGGAGAAATTGAAGATCATGAAAATGTAGACAATCAAGCCAAAACAGGCAATGCATTTTATGAGAATGAAGAAGACAGTAATTGCCAAAGAATTAAAAAAAATGAAAAAATAGAATACAATTCTTCAGACGAATTTTCAGACGATTCACTAATTGAACTATTGAACGAAACTCAAACACAAGTGGAACCTAACACTATTGAACAAGATTTAGACAAAGTGGAGAAAATGGTCTCTGACGATTTGAGAATTGCTACTGATTCCACTTTATCCGCATATGCTCTCAGAGCTAAGTCTGGTGCTCCAAGAGACGGCGTAGTACGATTAGTAATAGTAAGCTTACGTAGTGTTGAATTGCCAAAAATTGGAACGCAAAAAATACTAGAGTGTATAGACGGCAAAGGCGAACAAAGTTCTGTTGTCGTTCGACATCCTTGGGTATATTTGGAATTTGAGGTTGGGGATGTAATACATATTATAGAAGGGAAAAATATTGAAAACAAACGGCTTCTTTCTGACGACAAAAACCCTAAAACTCAACTGGCTAACGACAACCTGTTGGTGCTAAACCCTGATGTATTATTTTCGGCAACATCAGTCGGAAGTTCAGTAGGTTGTTTAAGACGTTCAATTCTGCAAATGCAATTTCAAGATCCCCGCGGAGAACCAAGCCTTGTCATGACTTTAGGCAATATCGTACACGAGTTATTGCAAGACTCAATCAAATACAAGCTCTCGCATAATAAAATTTCTATGGAAATCATAATACAAAAGCTGGATTCTTTGTTAGAAACTTATTCTTTTTCAATTATCATTTGTAATGAAGAAATCCAGTACGTTAAAGAATTAGTTATGAAAGAACATGCTGAAAACATTTTGTACTTCGTCAATAAATTTGTTTCGAAAAGTAACTATGGTTGCTACACATCAATTTCCGGAACAAGAAGAACACAGCCAATTTCTATATCCAATGTGATTGATATTGAAGAAAACATTTGGTCTCCAATATATGGTCTTAAAGGGTTCCTAGATGCAACTGTTGAAGCTAATGTTGAAAACAATAAAAAACACATCGTTCCTTTAGAAGTGAAAACTGGAAAATCCAGAAGCGTTTCATACGAGGTTCAAGGCCTTATTTATACACTTCTGTTAAATGATCGCTATGAGATACCGATAGAGTTTTTTCTTCTTTATTTTACGAGGGATAAAAATATGACAAAGTTCCCTTCTGTTCTGCACTCCATTAAACATATTCTCATGTCCCGAAATAGAATGAGTATGAATTTTAAGCATCAACTCCAAGAGGTTTTCGGACAGGCTCAATCAAGGTTTGAATTACCACCCTTACTGCGCGATTCATCTTGTGATTCATGTTTCATCAAAGAATCATGCATGGTGTTGAATAAGCTACTAGAGGACGGGACACCAGAAGAAAGTGGCCTGGTAGAAGGTGAGTTTGAAATTTTGACAAACCATTTGTCGCAAAATCTCGCCAACTACAAAGAGTTTTTTACAAAATATAATGATCTAATAACTAAGGAGGAGTCAAGTATAACATGTGTAAATAAAGAACTTTTCTTATTGGATGGTTCCACAAGAGAATCCAGAAGTGGAAGGTGCCTTTCTGGCTTGGTTGTGTCAGAGGTGGTTGAACATGAAAAAACAGAGGGAGCGTATATATATTGCTTCTCAAGACGTAGAAATGATAATAACTCGCAGTCGATGTTATCATCTCAAATTGCTGCTAATGACTTTGTTATTATTAGTGATGAGGAGGGTCATTTTTGCTTGTGCCAAGGTCGTGTACAATTTATAAATCCCGCTAAAATTGGTATCTCCGTAAAACGAAAGTTATTAAACAATAGGCTTCTAGATAAGGAAAAAGGAGTCACTACCATCCAAAGTGTGGTCGAATCAGAGCTAGAACAATCCAGTCTCATAGCTACCCAAAATTTGGTCACATATAGAATCGACAAAAATGATATCCAACAGAGTTTGTCTTTAGCGCGATTTAATCTTTTAAGCTTGTTTTTACCAGCTGTTTCACCTGGCGTGGATATTGTTGACGAAAGGAGTAAATTATGTCGGAAGACAAAGAGATCTGATGGAGGTAATGAAATACTGCGATCACTTTTAGTTGATAATAGAGCACCTAAGTTTAGAGATGCAAATGATGATCCCGTCATTCCGTATAAGCTTTCAAAGGATACAACATTGAACTTGAATCAAAAAGAAGCAATAGACAAAGTAATGAGGGCCGAAGATTATGCATTGATATTGGGAATGCCAGGGACTGGTAAGACTACTGTTATCGCAGAAATTATTAAAATTCTCGTTTCCGAGGGGAAACGCGTGCTTTTGACATCATACACACATTCAGCAGTGGATAATATTTTAATAAAATTGAGGAACACCAACATTAGCATAATGAGATTGGGAATGAAACATAAGGTCCATCCTGACACGCAAAAATATGTCCCGAATTACGCGTCAGTGAAAAGTTATAACGATTATTTGTCCAAAATTAACAGCACCTCTGTTGTTGCTACCACCTGCTTAGGAATTAACGATATACTATTTACACTAAATGAAAAGGATTTTGATTATGTGATTTTGGACGAGGCAAGTCAAATTTCAATGCCTGTCGCTTTGGGACCTCTGCGGTATGGAAATAGGTTTATTATGGTAGGAGATCATTACCAGCTACCGCCATTAGTTAAAAATGACGCAGCTCGATTAGGTGGGTTAGAAGAGTCACTGTTCAAGACTTTCTGTGAAAAGCATCCGGAAAGCGTTGCAGAACTGACTTTACAGTACCGTATGTGCGGCGATATTGTAACTTTGTCTAATTTCCTAATTTATGATAATAAATTAAAATGCGGGAATAACGAAGTTTTTGCTCAATCTTTAGAGTTACCAATGCCTGAGGCCTTGTCGCGCTATAGGAATGAATCGGCTAACTCTAAGCAGTGGCTTGAGGATATACTAGAGCCTACAAGAAAAGTGGTATTTTTAAATTACGATAACTGCCCGGATATTATCGAACAAAGCGAAAAGGATAACATTACGAATCACGGGGAGGCAGAATTGACTCTACAATGTGTGGAAGGAATGTTATTGAGCGGCGTACCATGCGAAGATATTGGTGTAATGACGTTATACAGAGCACAGTTAAGATTACTGAAGAAGATATTCAACAAAAATGTGTATGACGGGCTAGAGATCTTGACTGCTGATCAGTTTCAAGGTCGTGACAAAAAGTGCATTATTATTTCCATGGTTAGAAGAAATTCTCAATTAAATGGAGGAGCGCTGCTGAAAGAGCTAAGAAGAGTCAATGTTGCCATGACAAGGGCCAAATCCAAGCTGATCATTATAGGTTCAAAAAGTACAATCGGCTCTGTTCCTGAAATAAAATCGTTCGTGAACCTGTTAGAAGAGCGTAATTGGGTCTATACAATGTGCAAGGATGCACTCTACAAATACAAATTTCCCGACAGATCAAACGCTATTGATGAGGCACGCAAAGGTTGTGGAAAAAGAACAGGTGCCAAACCCATCACATCAAAGTCTAAATTCGTAAGCGATAAACCTATCATAAAGGAAATTCTACAAGAGTATGAAAGTCGTACGCTGCAGGTCGACTCCGGTTCTGCTGCTAGTGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGAAGAAGACTTGAACGGATCCTCTAGAGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGAAGAAGACTTGAACGGATCCACTAGCTAAAAAGTGGAACGATCATTCACTATATATATATCAATTTATATATACGTATGTGTAATTGAAGAAAGATACGTTTTTTCCTCTATTGAGAGGCCTGCTGGATGAATAGCTTTACCTTTTCTAAATCCTTGATACCATCAGTCTCTACTCCTCCACTTACATCGACACCGATCGCATTTGGTAACATATTAATGGCAACAGAAACGTTATCAGGATTCAATCCACCAGCGATAATGAATTTTATCTCGGGATGACTTGCAGACCAACTGGAAATTGCACTCCAATTCAATTTCTCACCAGTGCCACCTTCACCAGAATCGAACAACGTCAGCACATTGTCTACGTGTTCATACAGGTCCAGTAGTAATTCACAATCCTGTGGGAACTGGAACCTCTTAATGATTGGAATTGAAGATGGGATCAAAGATCTGTATTCTTTAATATCTTCATCTCCATGTAATTGTATCACATCTAGATTATATTCGTGGTACAGTTGAAGGACATCATCAACGGACTGATTTCTAAACACCCCGACTAGTTTAGTACCTTTCACGTTCTCTTGTTGGTGAACTGCAGTTGAAATACCTTTCGCAACAGATGAGTCAATGGTTCTTTTCCTACCGGGAACACAAATGATACCTAAGTAATCAGCACCATCATCCACAGCAGTCTTTGCAGCTTCAACGGTTTGCAAACCACACACTTTAACGAGCATCACAGTAGATTTCGCAAGAGGGTAGCTCGCTCAGAGTACCCAAGTAAATGATTAGTAAACTGATGTTTGATAGTTCAATTTTTCAATGAAATAACCTTATATTAAAATTGATATTACTATTATACAAAAATAAAGAATAAAGGATTTGAGTTTATACATAAAATACCATTATTATTTGTTCAGTGAGAGATACCGGGGTATATGGGATGTGTGTAGTGATACCATGCAATCATGTATCAAACATGGAAGAAGTGAAGGGGGAGAAAGAAACTTGAAAAAAAGAAAACCATTTGAAAAGAGGAAAAGTATCTAATTAAGATCTTTCACCTCTTAGTCTTCTGGCCTCGAGGCGTGCTCGCATTCATCGATTGACAGGGGGAATAAGAAGAGCTTCTCCATAACAGGAAAGCTATCACAGCATAAAATATCTATGTCATTTTATAAAGTTTGGTATATATAACACGTAATATTAGCATCTGAGGTTCGAAAGCTCGCATGTGTGTCTTTTCTAAAAAGTTTTCGTTTGAACTTTTCCAAAAGCTTCCTCATTAACGAACCAGGATGTTTTTTCACCAAATAATTTGTTAACCAATGCGGTTGGCAATTGATCGTTTTTCAAGTCAAAGATCTCATGCATTATATTTTGTTTGGAACTGCCCTCAGCCACAAAACACAGGGCTTTGGCGGGCCC

Amino Acid Sequence

MEEVIWKYSPLQRDMSDKTTSAAEYSDDYEDVQNPSSTPIVPNRLKTVLSFTNIQVPNADVNQLIQENGNEQVRPKPAEISTRESLRNIAAIADDAEGDLTIKPTITKFSDLPSSPIKAPNVEKKAEVNAEEVDKMDSTGDSNDGDDSLIDILTQKYVEKRKSESQITIQGNTNQKSGAQESCGKNDNTKSRGEIEDHENVDNQAKTGNAFYENEEDSNCQRIKKNEKIEYNSSDEFSDDSLIELLNETQTQVEPNTIEQDLDKVEKMVSDDLRIATDSTLSAYALRAKSGAPRDGVVRLVIVSLRSVELPKIGTQKILECIDGKGEQSSVVVRHPWVYLEFEVGDVIHIIEGKNIENKRLLSDDKNPKTQLANDNLLVLNPDVLFSATSVGSSVGCLRRSILQMQFQDPRGEPSLVMTLGNIVHELLQDSIKYKLSHNKISMEIIIQKLDSLLETYSFSIIICNEEIQYVKELVMKEHAENILYFVNKFVSKSNYGCYTSISGTRRTQPISISNVIDIEENIWSPIYGLKGFLDATVEANVENNKKHIVPLEVKTGKSRSVSYEVQGLIYTLLLNDRYEIPIEFFLLYFTRDKNMTKFPSVLHSIKHILMSRNRMSMNFKHQLQEVFGQAQSRFELPPLLRDSSCDSCFIKESCMVLNKLLEDGTPEESGLVEGEFEILTNHLSQNLANYKEFFTKYNDLITKEESSITCVNKELFLLDGSTRESRSGRCLSGLVVSEVVEHEKTEGAYIYCFSRRRNDNNSQSMLSSQIAANDFVIISDEEGHFCLCQGRVQFINPAKIGISVKRKLLNNRLLDKEKGVTTIQSVVESELEQSSLIATQNLVTYRIDKNDIQQSLSLARFNLLSLFLPAVSPGVDIVDERSKLCRKTKRSDGGNEILRSLLVDNRAPKFRDANDDPVIPYKLSKDTTLNLNQKEAIDKVMRAEDYALILGMPGTGKTTVIAEIIKILVSEGKRVLLTSYTHSAVDNILIKLRNTNISIMRLGMKHKVHPDTQKYVPNYASVKSYNDYLSKINSTSVVATTCLGINDILFTLNEKDFDYVILDEASQISMPVALGPLRYGNRFIMVGDHYQLPPLVKNDAARLGGLEESLFKTFCEKHPESVAELTLQYRMCGDIVTLSNFLIYDNKLKCGNNEVFAQSLELPMPEALSRYRNESANSKQWLEDILEPTRKVVFLNYDNCPDIIEQSEKDNITNHGEAELTLQCVEGMLLSGVPCEDIGVMTLYRAQLRLLKKIFNKNVYDGLEILTADQFQGRDKKCIIISMVRRNSQLNGGALLKELRRVNVAMTRAKSKLIIIGSKSTIGSVPEIKSFVNLLEERNWVYTMCKDALYKYKFPDRSNAIDEARKGCGKRTGAKPITSKSKFVSDKPIIKEILQEYESRTLQVDSGSAASGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGSSRGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGSTS*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU26149
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Linker between end of DNA2 and 6myc has been deleted.; The full cassette of DNA2 120-end D212A D213A L215A I218A 6myc KITRP1 3'UTR can be cut out Sac1 Apa1 and the full sequence of the cassette is in the sequence of insert field.; Internal Sac1 site KO silent mutation at nt5571 Internal EcoR1 site KO at nt 5577