cDNA Clone Pol1 (S.Pombe)

• Plasmid: pCE97 (pRS305 Gal1 promoter Pol1 1-328 Y40A Y48A S.Pombe ProtA 6HIS)
• Parent Plasmid: pRS305
• Expressed: Pol1 1-328 Y40A Y48A S.Pombe ProtA 6HIS
• Tag: 6His

Sequence of Insert

Full sequence of cassette
GCGGCCGCGACAGGTTATCAGCAACAACACAGTCATATCCATTCTCAATTAGCTCTACCACAGTGTGTGAACCAATGTATCCAGCACCACCTGTAACCAAAACAATTTTAGAAGTACTTTCACTTTGTAACTGAGCTGTCATTTATATTGAATTTTCAAAAATTCTTACTTTTTTTTTGGATGGACGCAAAGAAGTTTAATAATCATATTACATGGCATTACCACCATATACATATCCATATACATATCCATATCTAATCTTACTTATATGTTGTGGAAATGTAAAGAGCCCCATTATCTTAGCCTAAAAAAACCTTCTCTTTGGAACTTTCAGTAATACGCTTAACTGCTCATTGCTATATTGAAGTACGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAAAGTATCAACAAAAAATTGTTAATATACCTCTATACTTTAACGTCAAGGAGAAAAAACCCCTCTAGAACTAGGATGAGAAAGAGAAACGCGGGGATAAAGCCGTCTCAAAGATTTGCTGAGTTACGGGCTCTTCGTCAAGCCGGAAAAACAAGGGCTAGCACATACGAAAGCAAAGAAAATGAAGAATTAGCTGACAATGTTTCAGAGGAAGAAGCTCGCAAGATTGTTCGACAACGACTGGATGAGGATGACTTTGTCGTAGACGATAACGGGGCTGGTTATGTGGATAATGGATATGATGAGTGGGATCAAAGTCATTACAGTGACGAGGATGATGAAAATGAAAAAGGATCGTCTGGTCGAAAAAGAAAGAAATCGAAGAACGTTCTAGCTGAACCTAATCAACAAATTGGCGCGTTTTTTAAAGCAAATGCTATCCAACCTGCTTCACGTATCGCAAATCGAAAAAATAATGAGAAGGAAGATGAATTCATGGCTGAAATTCTTGGCTCAATCGACCAAGATATTCCCGAACGCCTCTCGACAAAAAAGGGAAATCGTTCTCATACTACATCAAATGCCAAACGTCGCTCTCAGAAAGCTGCTTCCTCAATAAGTACAACTAACGTTGTTTCTTCTGATCCCAAAAAATACATCCCAGAAACTGTACCTTCAACTCCTCAACCTGCTAGCAGTCTTCCTATTCCCAGCTCCCCACCTGCTGCACTCGAGACAGAAGAAAATGTCGACAGTCAACCAATGGAATTAGACGAACCAGAACTCCCCGTTGGATCTGATGCACCCATTATTGACGTTGAAGAATCTATTGCCATGAAGCCATTGATGAGTCATGATCAGGTCGATGACACCGCATCGCTTTCAAAAGCTACTATTTCTCAGCAACCTCTATCACCAATGACACCTTTATCTTCGGAACTTGACGTCTCTGCTTTTTCTGAAATTAATTCTAAGATAAAGAATGTAGATGTTCCTGCTTCGTCCTATTCATCTCCAATTTCAAAAGTATCACCATCTGATGTTACTGAAGAGGATGGTCGTACGCTGCAGGTCGACGGATCCCCTCAACAAAACAAAACCGCGGCTCTTGCGCAACACGATGAAGCCGTGGACAACAAATTCAACAAAGAACAACAAAACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAACAACGAAACGCCTTCATCCAAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAATGATGCTCAGGCGCCGAAAGTAGACAACAAATTCAACAAAGAACAACAAAACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAACAACGAAACGCCTTCATCCAAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAATGATGCTCAGGCGCCGAAAGTAGACGCGAATTCCCACCATCACCATCACCATCACTAG

Amino Acid Sequence

MRKRNAGIKPSQRFAELRALRQAGKTRASTYESKENEELADNVSEEEARKIVRQRLDEDDFVVDDNGAGYVDNGYDEWDQSHYSDEDDENEKGSSGRKRKKSKNVLAEPNQQIGAFFKANAIQPASRIANRKNNEKEDEFMAEILGSIDQDIPERLSTKKGNRSHTTSNAKRRSQKAASSISTTNVVSSDPKKYIPETVPSTPQPASSLPIPSSPPAALETEENVDSQPMELDEPELPVGSDAPIIDVEESIAMKPLMSHDQVDDTASLSKATISQQPLSPMTPLSSELDVSAFSEINSKIKNVDVPASSYSSPISKVSPSDVTEEDGRTLQVDGSPQQNKTAALAQHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDANSHHHHHHH*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU26156
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Pol1 can cut out Xba1 BamH1 ; Full gal1 promoter Pol1 1-328 PROTA 6HIS cassette can be cut out Not1 Kpn1.; Full sequence of cassette in the insert field