cDNA Clone Cyclin F

• Synonyms: CRISPR/Cas system-associated protein Cas9; cd09643, CCNF
• Plasmid: pBabeD hygro WT Cas9D-GGG-FLAG-CYCLIN F
• Parent Plasmid: pcDNA5D FRT/TO
• Expressed: Cas9D-GGG-FLAG-CYCLIN F
• Genbank: NM_001761.2
• Tag: FLAG
• Species: Human

Sequence of Insert

gaattcATGGGGAGCGGCGGCGTGGTCCACTGTAGGTGTGCCAAGTGTTT
CTGTTATCCTACAAAGCGAAGAATAAGGAGGAGGCCCCGAAACCTGACCA
TCTTGAGTCTCCCCGAAGATGTGCTCTTTCACATCCTGAAATGGCTTTCT
GTAGAGGACATCCTGGCCGTCCGAGCTGTACACTCCCAGCTGAAGGACCT
GGTGGACAACCACGCCAGTGTGTGGGCATGTGCCAGCTTCCAGGAGCTGT
GGCCGTCTCCAGGGAACCTGAAGCTCTTTGAAAGGGCTGCTGAAAAGGGG
AATTTCGAAGCTGCTGTGAAGCTGGGCATAGCCTACCTCTACAATGAAGG
CCTGTCTGTGTCTGATGAGGCCCGCGCAGAAGTGAATGGCCTGAAGGCCT
CTCGCTTCTTCAGTCTCGCTGAGCGGCTGAATGTGGGTGCCGCACCTTTC
ATCTGGCTCTTCATCCGCCCTCCGTGGTCGGTGAGCGGAAGCTGCTGCAA
GGCCGTGGTTCACGAGAGCCTCAGGGCAGAGTGCCAGCTGCAGAGGACTC
ACAAAGCATCCATATTGCACTGCTTGGGCAGAGTGCTGAGTCTGTTCGAG
GATGAGGAGAAGCAGCAGCAGGCCCATGACCTGTTTGAGGAGGCTGCTCA
TCAGGGATGTCTGACCAGCTCCTACCTCCTCTGGGAAAGCGACAGGAGGA
CAGATGTGTCAGATCCTGGGCGATGCCTCCACAGCTTCCGAAAACTCAGG
GACTACGCTGCCAAAGGCTGCTGGGAAGCGCAGCTGTCTTTAGCCAAAGC
CTGTGCAAATGCAAACCAGCTTGGACTGGAGGTGAGAGCTTCCAGTGAGA
TCGTCTGCCAGCTATTTCAGGCTTCCCAGGCTGTCAGTAAACAACAAGTC
TTCTCCGTGCAGAAGGGACTCAATGACACAATGAGGTACATTCTGATCGA
CTGGCTGGTGGAAGTTGCCACCATGAAGGACTTCACAAGCCTGTGCCTGC
ACCTGACCGTGGAGTGTGTGGACCGGTACCTGCGGAGGAGGCTGGTGCCG
CGGTACAGGCTCCAGCTGCTGGGCATCGCCTGCATGGTCATCTGCACCCG
GTTTATCAGTAAAGAGATCCTGACCATCCGGGAGGCCGTATGGCTCACGG
ACAACACTTACAAGTACGAGGACCTGGTGAGAATGATGGGCGAGATCGTC
TCCGCCTTGGAAGGGAAGATTCGAGTCCCCACTGTGGTGGATTACAAGGA
GGTCCTGCTGACGCTAGTCCCTGTGGAGCTGAGAACCCAGCACCTGTGCA
GCTTCCTCTGCGAGCTCTCCCTGCTGCACACCAGCCTGTCCGCCTACGCC
CCAGCCCGCCTGGCTGCCGCAGCCCTGCTCCTGGCCAGACTGACGCACGG
GCAGACACAGCCCTGGACCACTCAGCTGTGGGACCTCACCGGATTCTCCT
ATGAAGACCTCATTCCCTGCGTCTTGAGCCTCCATAAGAAGTGCTTCCAT
GATGACGCCCCCAAGGACTACAGGCAAGTCTCTCTGACCGCCGTGAAGCA
GCGGTTTGAGGACAAGCGCTATGGAGAAATCAGCCAGGAAGAGGTGCTGA
GCTACAGCCAGTTGTGTGCTGCATTAGGAGTGACACAAGACAGCCCCGAC
CCCCCGACTTTCCTCAGCACAGGGGAGATCCACGCCTTCCTCAGCTCTCC
CTCGGGGCGGAGAACCAAACGGAAGCGGGAGAACAGCCTCCAGGAAGACA
GAGGCAGCTTCGTTACCACCCCCACTGCGGAGCTGTCCAGCCAGGAGGAG
ACGCTGCTGGGCAGCTTCCTCGACTGGAGCCTGGACTGCTGCTCTGGCTA
TGAAGGCGACCAGGAGAGTGAGGGCGAGAAGGAGGGCGACGTGACAGCTC
CCAGCGGCATCCTCGATGTCACCGTGGTCTACCTGAACCCAGAACAGCAT
TGCTGCCAGGAATCCAGTGATGAGGAGGCTTGTCCAGAGGACAAGGGACC
CCAGGACCCACAGGCACTGGCGCTGGACACCCAGATCCCTGCAACCCCTG
GACCCAAACCCCTGGTCCGCACCAGCCGGGAGCCAGGGAAGGACGTCACG
ACCTCAGGGTACTCCTCCGTCAGCACCGCAAGTCCCACAAGCTCCGTGGA
CGGTGGCTTGGGGGCCCTGCCCCAACCTACCTCAGTGCTGTCCCTGGACA
GTGACTCGCACACACAGCCCTGCCACCATCAGGCCAGGAAGTCATGTTTA
CAGTGTCGTCCCCCAAGTCCCCCGGAGAGCAGTGTTCCCCAGCAACAGGT
GAAGCGGATAAACCTATGCATACACAGTGAGGAGGAGGACATGAACCTGG
GCCTTGTGAGGCTGTAAGCGGCCGAAGGGCGAATTCC

Amino Acid Sequence

MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGA
LLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHR
LEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKAD
LRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENP
INASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTP
NFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAI
LLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEI
FFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVD
LLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKI
IKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQ
LKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDD
SLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKV
MGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHP
VENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDD
SIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNL
TKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLI
REVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKK
YPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEI
TLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEV
QTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVE
KGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPK
YSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPE
DNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDK
PIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLSQLGGDSGGGDYKDDDDKEFMGSGGVVHCRCAKCFCYP
TKRRIRRRPRNLTILSLPEDVLFHILKWLSVEDILAVRAVHSQLKDLVDN
HASVWACASFQELWPSPGNLKLFERAAEKGNFEAAVKLGIAYLYNEGLSV
SDEARAEVNGLKASRFFSLAERLNVGAAPFIWLFIRPPWSVSGSCCKAVV
HESLRAECQLQRTHKASILHCLGRVLSLFEDEEKQQQAHDLFEEAAHQGC
LTSSYLLWESDRRTDVSDPGRCLHSFRKLRDYAAKGCWEAQLSLAKACAN
ANQLGLEVRASSEIVCQLFQASQAVSKQQVFSVQKGLNDTMRYILIDWLV
EVATMKDFTSLCLHLTVECVDRYLRRRLVPRYRLQLLGIACMVICTRFIS
KEILTIREAVWLTDNTYKYEDLVRMMGEIVSALEGKIRVPTVVDYKEVLL
TLVPVELRTQHLCSFLCELSLLHTSLSAYAPARLAAAALLLARLTHGQTQ
PWTTQLWDLTGFSYEDLIPCVLSLHKKCFHDDAPKDYRQVSLTAVKQRFE
DKRYGEISQEEVLSYSQLCAALGVTQDSPDPPTFLSTGEIHAFLSSPSGR
RTKRKRENSLQEDRGSFVTTPTAELSSQEETLLGSFLDWSLDCCSGYEGD
QESEGEKEGDVTAPSGILDVTVVYLNPEQHCCQESSDEEACPEDKGPQDP
QALALDTQIPATPGPKPLVRTSREPGKDVTTSGYSSVSTASPTSSVDGGL
GALPQPTSVLSLDSDSHTQPCHHQARKSCLQCRPPSPPESSVPQQQVKRI
NLCIHSEEEDMNLGLVRL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24803
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 24778. In-house codon optimised Cas9.