cDNA Clone LRRK2 (mouse)

• Synonyms: 4921513O20Rik, 9330188B09Rik, AW561911, D630001M17Rik, Gm927, cI-46
• Plasmid: pX335 LRRK2m KO antisense
• Parent Plasmid: pX335
• Expressed: LRRK2m KO antisense guide
• Genbank: NM_025730.3
• Species: Mouse

Sequence of Insert

GCTGACAGGCGCCACTGGCCA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48894
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR guide plasmid. Targets antisense strand in exon 1, designed to KO LRRK2m when co-transfected with sense guide 48891. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty flp-in line is preferable as this allows for downstream rescue and mutant analysis if necessary.