cDNA Clone KLHL3

• Synonyms: PHA2D
• Plasmid: pX335 KLHL3 KO antisense B
• Parent Plasmid: pX335
• Expressed: KLHL3 KO antisense guide B
• Genbank: ENSG00000146021
• Species: Human

Sequence of Insert

GCTGCAAATGCACGGATGCCC

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48898
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR guide plasmid. Targets antisense strand in exon 5, designed to KO KLHL3 when co-transfected with antisense guide 48899. Guide sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.