cDNA Clone RAB13

• Synonyms: GIG4
• Plasmid: pMK-RQ RAB13 Nter HA donor
• Parent Plasmid: pMK-RQ
• Expressed: RAB13 Nter KI sense guide
• Genbank: NM_005370.4
• Species: Human

Sequence of Insert

gcggccgCTCCAAAACTCAGCCCCATTTTTTTTTTTCAATTTCTAGTGCTCTCCCTACCTTCACCCTTATTCCCAAAACTCTTGGTTCTCTTCCCCTAATCCCTCTAAAATTTCTCTTCTAAAATTTCTGGTCGTCGTGGTTAAACCTCTACTTCCCTTCACCCCAGCCTCTCTGGCCTCAGAATGGAAGAGTAAGGTCCTAAGAGGGGAGCTACAATTACCGAAAGGCTGTGGAAAATAGCGAGAGAGACAGGCCGGCCTAAGCTAGGAAGGGAAAACCGAGAGAACAGAGGCCGGGCTGGCAGCCTTGGCCTGCTCAAAAGGGTGGGCGGGCAGGGGGTGGAGCCCGGAGGGGTGCCGCCTCTCCTCTTTCCCGGAGCCTGGGCGGAGAGGGAGGAAAACTTCTTCCTGGCCTGGGCTCCGTGCCGCTCTGTTTGCCAACCGTCCAGTCCCGCCTACCAGTGCCGGGCGCTCCCCACCCCTCCCCCGGCTCCCCCGGTGTCCGCCATGTACCCATACGATGTGCCAGATTACGCCGCgAAgGCaTACGAtCAtCTCTTCAAGctcCTGttaATaGGaGACTCGGGGGTGGGCAAGACTTGTCTGATCATTCGCTTTGCAGAGGACAACTTCAACAACACTTACATCTCCACCATCGGTGAGCCCGCTGGCCATGTCCCAGACCCCGACTCTCCTGTGACCTCCTTCTCCGGCTCCCGGATTACTAGTGACCCCACTTTTCAGTCCCCTTAGAGTAAGGCTCCTAAATTCTAGTCCCTCAACCCCTGGCTCTGACCCTTCCCCCGTGCATGTCTCTGTATAGTAACCAAACCTCTCACCTCTCTGAGGCCTTCAGGTAACTCTTCAACTGCTGCCAGCTCCTCATTTTCTTGGTACCATCGCCTTAGTCTCCCCAAATCCACAAGAACTCTTCTCTATTTTTCTCTGTTCCCCTCTTCCCCTACCCCCACCACCTAGAACTCTCTCGATCCTATCTCCATGTTTCCTGTATCCCTGTCCGCCACATCTTTTTCTCCCGATCCGATGCTAGCCTCTTTCTGTCTTGCTGAGCTTCAAACgcggccgc

• Vector Type: Other
• Antibiotic: Kan
• DU Number: DU48972
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR donor plasmid. Targets N-terminus of coding region, designed to KI an HA tag to the N-terminus of RAB13 when co-transfected with sense guide 48936 and antisense guide 48950. Donor sequence shown as sequence of insert, contains silent changes to prevent attack from Cas9. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line though an empty TRex line is preferable as this allows for downstream rescue and mutant analysis if necessary.