cDNA Clone RAB1A

• Synonyms: RAB1, YPT1
• Plasmid: pMK-RQ RAB1A Nter HA donor
• Parent Plasmid: pMK-RQ
• Genbank: NM_004161.4
• Species: Human

Sequence of Insert

gcggCCGCCAGGGGGAGTCCGAGCGAAGTCCTAGCCAGCGAGTCAGAGGGGAGGGGAGCAGGGAGGGGCCGAGGGTGGGGAGGTGAGGGAGTGGGGAATGGGGCGGGCGACAACCCTTCAGGTACGCATGCCCCAGAGGCGCGGCGCTTGGCGGGAAGCTGAGTCCTGGCCTTGCGTCGCACTGTCTGTCCTCAGCTCGCGTAGCCGCGCTCGCGACTCCCTTTCCCGGCATGCCAGGCGGTGCGGCCGCCCTCTGGGCCGTGTAAAGGCCCCTCGGTCTAAGGCTTCCCTATTTCCTGGTTCGCCGGCGGCCATTTTGGGTGGAAGCGATAGCTGAGTGGCGGCGGCTGCTGATTGTGTTCTAGGGGACGGAGTAGGGGAAGACGTTTGCTCTCCCGGAACAGCCTATCTCATTCCTTTCTTTCGATTACCCGTGGCGCGGAGAGTCAGGGCGGCGGCTGCGGCAGCAAGGGCGGCGGTGGCGGCGGCGGCAGCTGCAGTGACATGTACCCATACGATGTGCCAGATTACGCCagtAGCATGAATCCCGAATAGTGAGTTCAGGAGAGCACCaaTCGGCTGGGTCCGTGGGCCAGCTTGGGGGATCTTAAAGGGGTCGAGGAGGGTTGGGGCAGAAGTCGGGGCATCGGCTGGGGTGAGGCGAGGGTGATGGGTCAGGAGAGGCTGGCGGCCGGGAGTCGGGCCCCATTGTCTGACGCGGAGGGGCGGCCGCGCGGGGGAGGGGTCGGGCCGGAGGGGTGAGCCGCCCGGGCCTGGACCGGGTCAGGTTAGAGGGCCTGACTGCGGGGCGGGTGCTGAGGAAGCCTGCCGAGGGGCCTGGGGCGGTGTGAAGGGGTATCTTCTCTCGGAGGCAGTGACTTTTGAAGGAGGACTTGTCTCTAAGGGGAGGGGATGGGGTGGGAGAGCCCTTCTAGAGGGCACTGTCAGACCCTGCGCCCGCACTCTGCGGAGCTGTCAGGATCTTCGGGGTAGAAACCAGCTTTACTTGTAAATCCTGAGCTTGTTGGCTCTCTCTCCTTCCATCCTCCCCGCCAGGTTTCAGGTAATATGGATGCggccgc

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU48990
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR donor plasmid. Designed to KI an HA tag to the N-terminus of RAB1A when co-transfected with guides 48937 and 48951. Donor sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting and FACs. Note this system does not require induction with Tet/Dox. May be used in any cell-line, contains several silent mutations to prevent binding of guides.