cDNA Clone USP24

• Plasmid: pcDNA5 FRT/TO GFP USP24 C1538A
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP USP24 C1538A
• Genbank: Gene 23358 NP_056121.2 NM_015306.2 (no ncbi ref available for variant 2)
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGCCGCTATGACCACGCTGCTGTGCATGGGCTTCTCAGACCCCGCCAC
CATCCGCAAGGCCCTGCGCCTGGCCAAGAACGACATTAACGAGGCCGTGG
CACTGCTCACCAACGAGCGGCCGGGCCTCGACTACGGCGGCTACGAGCCC
ATGGACAGCGGCGGTCTTTCCGAGTCTGATGAGAATTGTAGAAGGTTTAT
GGACAGGTGTATGCCTGAAGCATTTAAAAAGCTCCTGACATCAAGTGCTG
TTCACAAGTGGGGTACTGAAATTCATGAAGGAATTTACAACATGTTGATG
CTATTAATAGAACTGGTCGCAGAGAGAATAAAACAAGATCCAATTCCCAC
TGGTCTCCTGGGTGTGCTTACAATGGCTTTCAATCCTGATAATGAATACC
ATTTTAAAAACAGAATGAAAGTGTCTCAAAGGAATTGGGCAGAAGTGTTT
GGAGAGGGAAATATGTTTGCTGTTTCACCTGTATCGACTTTCCAAAAGGA
GCCTCATGGATGGGTTGTGGATTTGGTAAATAAGTTTGGAGAATTAGGTG
GATTTGCAGCAATCCAAGCCAAGCTCCATTCAGAAGATATAGAACTTGGG
GCTGTCTCAGCACTGATTCAGCCCTTAGGAGTGTGTGCAGAGTACCTCAA
TTCCTCCGTGGTACAGCCCATGCTAGACCCAGTCATTCTTACTACAATCC
AGGATGTACGGAGTGTAGAAGAGAAAGACCTCAAAGACAAGAGATTGGTT
AGCATCCCTGAGCTCTTGTCTGCCGTTAAGTTACTTTGCATGCGCTTCCA
ACCGGATCTGGTGACAATTGTGGATGACCTTCGACTAGATATTCTATTGC
GCATGCTGAAATCACCACATTTCAGTGCTAAGATGAATTCTCTCAAAGAA
GTAACCAAACTAATAGAAGATAGCACTTTATCCAAATCTGTGAAGAATGC
TATAGATACAGACAGATTATTAGATTGGCTAGTTGAAAACTCAGTTCTGT
CGATTGCACTGGAAGGCAACATAGACCAAGCACAATACTGTGACCGTATA
AAGGGAATTATTGAACTCTTGGGTAGTAAATTGTCGTTAGATGAACTCAC
TAAAATTTGGAAGATACAGTCAGGACAATCATCTACTGTGATTGAGAACA
TTCATACTATTATTGCTGCAGCGGCTGTGAAATTTAATTCAGATCAGCTT
AATCATTTGTTTGTTCTCATTCAGAAGGTTTTAGACGTACTCTGGGAACT
GGCTCACCTTCCAACCCTGCCCAGTAGCCTTATTCAGCAGGCCTTGGAGG
AGCACCTGACAATCCTTAGTGATGCATATGCAGTGAAAGAAGCAATCAAG
AGGAGCTACATCATCAAGTGCATAGAAGATATTAAGAGGTCATCTCAGCT
TAATAATCCCCAGTTTGTATGGGTGGTACCAGCTTTGCGTCAGCTCCATG
AAATTACTCGCTCATTCATAAAACAAACCTATCAAAAGCAAGACAAGAGC
ATTATTCAAGACTTGAAGAAGAATTTTGAAATAGTGAAATTGGTAACGGG
AAGTTTGATCGCTTGTCATCGGCTTGCAGCTGCTGTGGCCGGGCCTGGAG
GCTTAAGTGGCTCGACACTAGTGGATGGCCGGTACACTTACCGGGAGTAT
TTAGAGGCACATCTAAAATTTCTAGCGTTTTTCTTGCAAGAAGCTACTCT
GTATCTGGGCTGGAATCGTGCCAAGGAGATCTGGGAGTGTCTTGTAACTG
GCCAGGATGTTTGTGAATTAGATAGAGAGATGTGTTTTGAATGGTTTACA
AAAGGACAGCATGATCTTGAGAGTGATGTTCAGCAGCAGCTCTTCAAGGA
GAAAATTCTTAAATTGGAGTCATATGAAATCACTATGAATGGTTTTAACT
TATTTAAAACTTTTTTTGAAAATGTGAATCTTTGTGATCATCGATTGAAA
AGACAAGGAGCTCAGTTGTATGTAGAAAAGCTGGAATTGATAGGAATGGA
TTTCATTTGGAAAATAGCCATGGAATCACCTGATGAAGAAATTGCTAATG
AAGCTATTCAGCTAATCATAAACTATAGTTACATTAATCTAAATCCTAGA
TTAAAGAAGGATTCAGTATCTTTACATAAGAAATTCATTGCTGATTGCTA
CACAAGATTAGAAGCAGCCAGTTCAGCACTTGGTGGCCCCACTCTAACAC
ATGCTGTGACCAGAGCAACAAAAATGCTTACAGCAACTGCCATGCCAACT
GTAGCAACCTCAGTTCAGTCTCCTTATAGATCTACTAAACTTGTAATAAT
TGAGAGATTGCTGCTTCTGGCAGAGCGCTATGTGATCACTATAGAGGATT
TTTACTCTGTTCCACGAACTATTCTACCTCATGGTGCCTCATTTCATGGA
CATCTTTTAACCCTTAATGTTACCTATGAGTCTACCAAAGATACCTTCAC
TGTCGAGGCTCACAGTAATGAAACCATAGGGAGTGTCCGGTGGAAAATAG
CCAAGCAGTTGTGCTCTCCTGTGGATAATATACAGATATTTACAAATGAT
AGCCTGCTGACAGTGAATAAAGATCAAAAGCTACTCCACCAACTGGGCTT
TTCTGATGAACAAATCCTTACAGTGAAGACTTCTGGCAGTGGGACCCCAT
CTGGGAGTTCAGCAGATTCTTCAACCAGCTCCAGCAGCAGCAGCAGTGGG
GTTTTTAGTTCTTCATATGCCATGGAGCAGGAGAAATCCCTCCCTGGTGT
AGTGATGGCTCTCGTATGTAACGTATTTGACATGCTTTATCAGCTCGCCA
ATCTGGAAGAGCCAAGGATAACTCTACGAGTACGGAAGCTTCTGCTCTTG
ATACCCACTGATCCAGCCATTCAGGAAGCCCTTGATCAACTTGATTCTTT
AGGAAGAAAGAAAACATTGCTGTCTGAATCAAGTTCTCAGTCCTCAAAAT
CTCCATCCCTGTCATCAAAGCAACAGCACCAGCCAAGTGCCAGTTCAATT
TTAGAAAGTCTGTTTCGATCTTTTGCCCCGGGAATGTCTACCTTCAGAGT
GCTCTACAACTTAGAAGTTCTAAGCTCCAAACTCATGCCAACAGCTGATG
ATGACATGGCCAGAAGCTGTGCCAAATCCTTCTGTGAAAACTTCCTCAAA
GCTGGCGGTTTGAGTTTGGTTGTAAATGTCATGCAGAGAGACTCCATCCC
ATCAGAAGTAGACTATGAAACAAGGCAGGGTGTTTATTCCATCTGTCTAC
AGCTTGCAAGATTTTTACTTGTCGGACAAACAATGCCCACGTTATTAGAT
GAAGACCTCACCAAAGATGGTATAGAAGCACTTTCTTCCCGCCCATTCCG
AAATGTCAGCCGGCAGACAAGCAGACAGATGTCCTTATGTGGTACCCCAG
AAAAGTCATCCTACCGACAGTTGTCCGTGTCTGATAGGTCTTCTATTAGG
GTTGAGGAAATCATCCCTGCTGCTCGAGTTGCAATACAAACAATGGAAGT
AAGTGATTTCACTTCTACTGTGGCTTGCTTCATGAGATTGTCATGGGCTG
CGGCTGCAGGACGGCTTGATCTTGTTGGGAGTAGCCAGCCAATTAAAGAA
AGTAATTCCCTGTGTCCTGCTGGAATTCGAAACAGACTCAGCAGTTCAGG
AAGCAATTGCAGCTCTGGAAGTGAAGGAGAACCAGTAGCCCTGCATGCGG
GAATCTGTGTTCGACAACAGTCTGTATCCACCAAAGACTCGCTGATTGCG
GGAGAGGCTTTGTCTCTTCTTGTTACGTGCCTACAGCTTCGGAGCCAGCA
ACTGGCATCTTTCTATAACTTGCCCTGTGTTGCTGATTTCATCATTGATA
TTCTGCTCGGATCACCAAGTGCTGAGATTCGCCGGGTTGCCTGTGATCAG
CTGTACACTCTTAGTCAGACAGACACATCAGCGCATCCAGATGTGCAGAA
GCCAAATCAGTTTCTTCTAGGCGTAATCCTCACGGCTCAGCTGCCTCTCT
GGTCTCCAACTAGTATTATGAGAGGAGTCAATCAGAGACTGTTATCTCAG
TGTATGGAGTATTTTGATTTGAGATGCCAGTTATTAGATGATCTGACAAC
TTCAGAAATGGAGCAGTTAAGGATCAGCCCAGCTACGATGCTTGAAGATG
AGATTACTTGGCTGGATAACTTTGAACCTAATCGTACAGCTGAATGTGAG
ACCAGTGAAGCGGACAACATCTTACTGGCAGGGCACTTACGCCTCATCAA
GACCCTTCTTTCACTCTGTGGGGCAGAAAAGGAAATGCTTGGTTCATCAC
TCATTAAACCATTGTTAGATGACTTCCTTTTCCGAGCTTCTAGAATTATT
TTAAATAGTCATTCTCCAGCTGGCAGTGCCGCCATCAGTCAACAGGACTT
TCATCCAAAGTGTAGTACAGCGAATAGCCGATTGGCAGCCTATGAAGTCC
TTGTGATGTTGGCTGATAGTTCACCTTCAAATCTTCAAATTATTATAAAA
GAACTGCTTTCTATGCATCACCAGCCTGACCCTGCTCTTACCAAGGAGTT
TGATTACCTTCCCCCAGTGGATAGCAGGTCCAGTTCAGGGTTTGTGGGGC
TGAGAAATGGTGGTGCAACTgcTTATATGAATGCAGTCTTCCAGCAGCTG
TATATGCAACCTGGGCTCCCTGAGTCATTACTTTCAGTGGATGATGACAC
AGACAATCCAGATGATAGCGTGTTTTACCAAGTGCAGTCTCTCTTTGGAC
ATTTAATGGAAAGCAAGCTGCAGTACTATGTACCTGAGAATTTTTGGAAG
ATTTTCAAGATGTGGAATAAAGAACTTTATGTGAGAGAACAGCAGGATGC
ATATGAATTCTTTACTAGTCTCATTGATCAGATGGATGAATACCTCAAGA
AAATGGGGAGAGACCAAATTTTTAAGAATACATTTCAGGGCATCTACTCT
GATCAGAAGATCTGTAAAGACTGTCCTCACAGATATGAGCGTGAAGAAGC
TTTCATGGCTCTCAATCTAGGAGTGACTTCTTGTCAGAGTTTGGAAATTT
CTTTGGACCAATTTGTTAGAGGAGAAGTTCTAGAAGGAAGTAATGCGTAC
TACTGTGAAAAGTGTAAAGAAAAGAGAATAACAGTGAAAAGGACCTGTAT
TAAATCTTTACCTAGCGTCTTGGTAATTCACCTAATGAGATTTGGGTTTG
ACTGGGAAAGCGGACGCTCCATTAAATATGATGAACAAATAAGGTTTCCC
TGGATGCTAAACATGGAGCCTTACACAGTTTCAGGAATGGCTCGCCAAGA
TTCTTCTTCTGAAGTTGGGGAAAATGGGCGAAGTGTGGATCAGGGCGGTG
GAGGATCCCCACGAAAAAAGGTTGCCCTCACAGAAAACTATGAACTTGTC
GGTGTCATCGTACACAGTGGGCAGGCACACGCAGGCCACTACTATTCCTT
CATTAAGGACAGGCGAGGGTGTGGAAAAGGAAAGTGGTATAAATTTAATG
ACACAGTTATAGAAGAATTTGACCTAAATGACGAGACCCTGGAGTATGAA
TGCTTTGGAGGAGAATATAGACCAAAAGTTTATGATCAAACAAACCCATA
CACTGATGTGCGCCGAAGATACTGGAATGCCTATATGCTTTTCTACCAAA
GGGTGTCTGATCAGAACTCCCCAGTATTACCAAAGAAAAGTCGAGTCAGC
GTTGTACGGCAGGAAGCTGAGGATCTCTCTCTGTCAGCTCCATCTTCACC
AGAAATTTCACCTCAGTCATCCCCTCGGCCCCATAGGCCGAACAATGACC
GGCTGTCTATTCTTACCAAGCTGGTTAAAAAAGGCGAGAAGAAAGGACTG
TTTGTGGAGAAAATGCCTGCTCGAATATACCAGATGGTGAGAGATGAGAA
CCTCAAGTTTATGAAGAATAGAGATGTATACAGTAGTGATTATTTCAGTT
TTGTTTTGTCTTTAGCTTCATTGAATGCTACTAAATTAAAGCATCCATAT
TATCCTTGCATGGCAAAGGTGAGCTTACAGCTTGCTATTCAATTCCTTTT
TCAAACTTATCTACGGACAAAGAAGAAACTCAGGGTTGATACTGAAGAAT
GGATTGCTACCATTGAAGCATTGCTTTCAAAAAGTTTTGATGCTTGTCAG
TGGTTAGTTGAATATTTTATTAGTTCTGAAGGACGAGAATTGATAAAGAT
TTTCTTACTGGAGTGTAATGTGAGAGAAGTACGAGTTGCTGTGGCCACCA
TTCTGGAGAAAACCCTAGACAGTGCCTTGTTTTATCAGGATAAGTTAAAA
AGCCTTCATCAGTTACTGGAGGTACTACTTGCTCTGTTGGACAAAGACGT
CCCAGAAAATTGTAAAAACTGTGCTCAGTACTTTTTCCTGTTCAACACTT
TTGTACAAAAGCAAGGAATTAGGGCTGGAGATCTTCTTCTGAGGCATTCA
GCTCTGCGGCACATGATCAGCTTCCTCCTAGGGGCCAGTCGGCAAAACAA
TCAGATACGTCGATGGAGTTCAGCACAAGCACGAGAATTTGGGAATCTTC
ACAATACAGTGGCGTTACTTGTTTTGCATTCAGATGTCTCATCCCAAAGG
AATGTTGCTCCTGGCATATTTAAGCAACGACCACCCATTAGCATTGCTCC
CTCAAGCCCTCTGTTGCCCCTCCATGAGGAGGTAGAAGCCTTGTTGTTCA
TGTCTGAAGGGAAACCTTACCTGTTAGAGGTAATGTTTGCTTTGCGGGAG
CTGACAGGCTCGCTCTTGGCACTCATTGAGATGGTAGTGTACTGCTGTTT
CTGTAATGAGCATTTTTCCTTCACAATGCTGCATTTCATTAAGAACCAAC
TAGAAACGGCTCCACCTCATGAGTTAAAGAATACGTTCCAACTACTTCAT
GAAATATTGGTTATTGAAGATCCTATACAAGTAGAGCGAGTCAAATTTGT
GTTTGAGACAGAAAATGGATTACTAGCTTTGATGCACCACAGTAATCATG
TGGACAGTAGTCGCTGCTACCAGTGTGTCAAATTTCTTGTCACTCTTGCT
CAAAAGTGTCCTGCAGCTAAGGAGTACTTCAAGGAGAATTCCCACCACTG
GAGCTGGGCTGTGCAGTGGCTACAGAAGAAGATGTCAGAACATTACTGGA
CACCACAGAGTAATGTCTCTAATGAAACATCAACTGGAAAAACCTTTCAG
CGAACCATTTCAGCTCAGGACACGTTAGCGTATGCCACAGCTTTGTTGAA
TGAAAAAGAGCAATCAGGAAGCAGTAATGGGTCGGAGAGTAGTCCTGCCA
ATGAGAACGGAGACAGGCATCTACAGCAGGGTTCAGAATCTCCCATGATG
ATTGGTGAGTTGAGAAGTGACCTTGATGATGTTGATCCCTAGGgGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSRKGTI
DAAAMTTLLCMGFSDPATIRKALRLAKNDINEAVALLTNERPGLDYGGYE
PMDSGGLSESDENCRRFMDRCMPEAFKKLLTSSAVHKWGTEIHEGIYNML
MLLIELVAERIKQDPIPTGLLGVLTMAFNPDNEYHFKNRMKVSQRNWAEV
FGEGNMFAVSPVSTFQKEPHGWVVDLVNKFGELGGFAAIQAKLHSEDIEL
GAVSALIQPLGVCAEYLNSSVVQPMLDPVILTTIQDVRSVEEKDLKDKRL
VSIPELLSAVKLLCMRFQPDLVTIVDDLRLDILLRMLKSPHFSAKMNSLK
EVTKLIEDSTLSKSVKNAIDTDRLLDWLVENSVLSIALEGNIDQAQYCDR
IKGIIELLGSKLSLDELTKIWKIQSGQSSTVIENIHTIIAAAAVKFNSDQ
LNHLFVLIQKVLDVLWELAHLPTLPSSLIQQALEEHLTILSDAYAVKEAI
KRSYIIKCIEDIKRSSQLNNPQFVWVVPALRQLHEITRSFIKQTYQKQDK
SIIQDLKKNFEIVKLVTGSLIACHRLAAAVAGPGGLSGSTLVDGRYTYRE
YLEAHLKFLAFFLQEATLYLGWNRAKEIWECLVTGQDVCELDREMCFEWF
TKGQHDLESDVQQQLFKEKILKLESYEITMNGFNLFKTFFENVNLCDHRL
KRQGAQLYVEKLELIGMDFIWKIAMESPDEEIANEAIQLIINYSYINLNP
RLKKDSVSLHKKFIADCYTRLEAASSALGGPTLTHAVTRATKMLTATAMP
TVATSVQSPYRSTKLVIIERLLLLAERYVITIEDFYSVPRTILPHGASFH
GHLLTLNVTYESTKDTFTVEAHSNETIGSVRWKIAKQLCSPVDNIQIFTN
DSLLTVNKDQKLLHQLGFSDEQILTVKTSGSGTPSGSSADSSTSSSSSSS
GVFSSSYAMEQEKSLPGVVMALVCNVFDMLYQLANLEEPRITLRVRKLLL
LIPTDPAIQEALDQLDSLGRKKTLLSESSSQSSKSPSLSSKQQHQPSASS
ILESLFRSFAPGMSTFRVLYNLEVLSSKLMPTADDDMARSCAKSFCENFL
KAGGLSLVVNVMQRDSIPSEVDYETRQGVYSICLQLARFLLVGQTMPTLL
DEDLTKDGIEALSSRPFRNVSRQTSRQMSLCGTPEKSSYRQLSVSDRSSI
RVEEIIPAARVAIQTMEVSDFTSTVACFMRLSWAAAAGRLDLVGSSQPIK
ESNSLCPAGIRNRLSSSGSNCSSGSEGEPVALHAGICVRQQSVSTKDSLI
AGEALSLLVTCLQLRSQQLASFYNLPCVADFIIDILLGSPSAEIRRVACD
QLYTLSQTDTSAHPDVQKPNQFLLGVILTAQLPLWSPTSIMRGVNQRLLS
QCMEYFDLRCQLLDDLTTSEMEQLRISPATMLEDEITWLDNFEPNRTAEC
ETSEADNILLAGHLRLIKTLLSLCGAEKEMLGSSLIKPLLDDFLFRASRI
ILNSHSPAGSAAISQQDFHPKCSTANSRLAAYEVLVMLADSSPSNLQIII
KELLSMHHQPDPALTKEFDYLPPVDSRSSSGFVGLRNGGATAYMNAVFQQ
LYMQPGLPESLLSVDDDTDNPDDSVFYQVQSLFGHLMESKLQYYVPENFW
KIFKMWNKELYVREQQDAYEFFTSLIDQMDEYLKKMGRDQIFKNTFQGIY
SDQKICKDCPHRYEREEAFMALNLGVTSCQSLEISLDQFVRGEVLEGSNA
YYCEKCKEKRITVKRTCIKSLPSVLVIHLMRFGFDWESGRSIKYDEQIRF
PWMLNMEPYTVSGMARQDSSSEVGENGRSVDQGGGGSPRKKVALTENYEL
VGVIVHSGQAHAGHYYSFIKDRRGCGKGKWYKFNDTVIEEFDLNDETLEY
ECFGGEYRPKVYDQTNPYTDVRRRYWNAYMLFYQRVSDQNSPVLPKKSRV
SVVRQEAEDLSLSAPSSPEISPQSSPRPHRPNNDRLSILTKLVKKGEKKG
LFVEKMPARIYQMVRDENLKFMKNRDVYSSDYFSFVLSLASLNATKLKHP
YYPCMAKVSLQLAIQFLFQTYLRTKKKLRVDTEEWIATIEALLSKSFDAC
QWLVEYFISSEGRELIKIFLLECNVREVRVAVATILEKTLDSALFYQDKL
KSLHQLLEVLLALLDKDVPENCKNCAQYFFLFNTFVQKQGIRAGDLLLRH
SALRHMISFLLGASRQNNQIRRWSSAQAREFGNLHNTVALLVLHSDVSSQ
RNVAPGIFKQRPPISIAPSSPLLPLHEEVEALLFMSEGKPYLLEVMFALR
ELTGSLLALIEMVVYCCFCNEHFSFTMLHFIKNQLETAPPHELKNTFQLL
HEILVIEDPIQVERVKFVFETENGLLALMHHSNHVDSSRCYQCVKFLVTL
AQKCPAAKEYFKENSHHWSWAVQWLQKKMSEHYWTPQSNVSNETSTGKTF
QRTISAQDTLAYATALLNEKEQSGSSNGSESSPANENGDRHLQQGSESPM
MIGELRSDLDDVDP*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5 FRT/TO GFP USP24 C1538A90.1 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU24849
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: transcript variant 2 - comparedto NCBI ref has 8 residues missing at the extreme N-terminus plus 3 internal deletions (aa61-164) (aa519-550) and (aa605-620). C1538A in variant 2 corresponds to C1698A in NCBI ref. Clone also has a silent mutation at C2069 compared to NCBI ref and a faulty 3' Not1 site
• Low Temp: No