cDNA Clone KLHL3

• Synonyms: PHA2D
• Plasmid: pMK-RQ KLHL3 Cter GFP donor
• Parent Plasmid: pMK-RQ
• Genbank: ENSG00000146021
• Species: Human

Sequence of Insert

ATAGTGCAGGTTGACTGGTGGCAGAGCTAGGAATGGAATTCAGTCTTTCTTGCTCCCATGAAGGCAGCCTGCTGTACTTTCCGCTTCATTCCAGTGTCTTCTAAAAGTAGAGATGAATCACCCATTGCTTCTCTTACAGTGATTCAAATTTAGAACTGCATAGCTGCCCCTGGGCGCTGAGCATTGTTGGGCTGGGTGCTAGGGCTGGGGATGGGAGTCACCACAGTGAGTCCATGTGATGACACAGGGTGAGGCCACTAGTCAGTGGTGTTTGAGGGACTGTGCTCGTAGGTTCAAGTGTCTTGCCAGGACTTGGCTGTTGGAGTGGGGAACAGCCAGCTGCTAGCAGCCTGAGTCCACTTCAATATTAGGATAAAAATTGCACACATTTCCCTTGGCTGTGACTCAGGATATCTTGGATTCATGAACTCACTGGGACTCTGAGTAATTTTGGAGAAGCTAAGATGATAACTTTCTCCCCCTTTCTTATTAGGTGTTGCaGTGATTCACAAGTCCTTGGGTGGAGGCGGAGGTgtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagTGACCCAAACTCCTACTGCCAGGActTGGAGGAAGGAGCAGGTGCTGCCTGTGACTCTGAACAGCAGGACCTTGGTGACTGGATTCAACTTGCTTGGGAGGGTCTGTGCTGCTGTGAGAACCGCTCTCCTCTGACTTGGCAGACTGGTGTTGTTCATCGCAGTGTGGACACCATTACCCACCCCCGTTCCCCTGAGGTGCTCTGGCCTATGCCCTGAGCAAGGGGGGTCTTGACATCCCCAGGCAGCACCTTTGGGCTTTGTTTTGGTGTTTCTACAGGGACAATACAGACCCTGGAGTGTGTGTGTGTGTGTGTGTGTGTGTAGACCATGGTGTTTCTCTATGTTTCTCTAAGTTGGGGGGTGAGCGTGTGTGACAGTCTACTGGATTTCTTTACTACTGATCCTTTCGCTGTGTTAAAAATCAAGTCACAGAGACCTCTCTTCTGGATTTGTCCCATGGGGACCCTGAGACTACTAAAGCTGCTTTCTTCTGAAGGTCCAGTTGGACAGTCTGGGAATGTCCAG

• Vector Type: Other
• Antibiotic: Kan
• DU Number: DU52069
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR donor plasmid. Designed to KI a c-terminal GFP reporter to KLHL3 when co-transfected with guides 48975 and 48979. Donor sequence shown as sequence of insert. Positives may be enriched for with puromycin prior to further selection by western blotting. Note this system does not require induction with Tet/Dox and may be used in any cell-line.