cDNA Clone HOIP

• Synonyms: ring finger protein 31 (RNF31) (HOIP)
• Plasmid: pcDNA5-FRT/TO-GFP HOIP (delta Xho1)
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-HOIP
• Genbank: NM_017999.4
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGCCGCGATGCCGGGGGAGGAAGAGGAGCGGGCCTTCCTGGTGGCCCG
CGAGGAGCTGGCGAGCGCCCTGAGGAGGGATTCCGGGCAGGCGTTTTCCC
TGGAGCAGCTCCGGCCGCTACTAGCCAGCTCTCTGCCGCTAGCCGCCCGC
TACCTGCAGCTGGACGCCGCACGCCTTGTCCGCTGCAACGCTCATGGGGA
GCCCCGAAACTACCTCAACACCCTGTCCACGGCTCTGAACATCCTGGAGA
AATACGGCCGCAACCTTCTCAGCCCTCAGCGGCCTCGGTACTGGCGTGGT
GTCAAGTTTAATAACCCTGTCTTTCGCAGCACGGTGGATGCTGTGCAGGG
GGGCCGAGATGTGCTGCGATTATATGGCTACACAGAGGAGCAACCAGATG
GGTTGAGCTTCCCCGAAGGGCAGGAGGAGCCAGATGAGCACCAGGTTGCT
ACAGTCACACTGGAAGTACTGCTGCTTCGGACAGAGCTCAGCCTGCTATT
GCAGAATACTCATCCAAGACAGCAGGCACTGGAGCAGCTGTTGGAAGACA
AGGTTGAAGATGATATGCTGCAGCTTTCAGAATTTGACCCCCTATTGAGA
GAGATTGCTCCTGGCCCCCTCACCACACCCTCTGTCCCAGGCTCCACTCC
TGGTCCCTGCTTCCTCTGTGGTTCTGCCCCAGGCACACTGCACTGCCCAT
CCTGTAAACAGGCCCTGTGTCCAGCCTGTGACCACCTGTTCCATGGACAC
CCATCCCGTGCTCATCACCTCCGCCAGACCCTGCCTGGGGTCCTGCAGGG
TACCCACCTGAGCCCCAGTTTACCTGCCTCAGCCCAACCACGGCCCCAGT
CGACCTCCCTGCTGGCCCTGGGAGACAGCTCTCTTTCTTCCCCTAATCCT
GCAAGTGCTCATTTGCCCTGGCACTGTGCTGCCTGTGCCATGCTAAATGA
GCCTTGGGCAGTGCTCTGTGTGGCCTGTGATCGGCCCCGAGGCTGTAAGG
GGTTGGGGTTGGGAACTGAGGGTCCCCAAGGAACTGGAGGCCTAGAACCT
GATCTTGCACGGGGTCGGTGGGCCTGCCAGAGCTGTACCTTTGAGAATGA
GGCAGCTGCTGTGCTATGTTCCATATGTGAGCGACCTCGGCTGGCCCAGC
CTCCCAGCTTGGTGGTGGATTCCCGAGATGCTGGCATTTGCCTGCAACCC
CTTCAGCAGGGGGATGCTTTGCTGGCCTCTGCCCAGAGTCAAGTCTGGTA
CTGTATTCACTGTACCTTCTGCAACTCGAGCCCTGGCTGGGTGTGTGTTA
TGTGCAACCGGACTAGTAGCCCCATTCCAGCACAACATGCCCCCCGGCCC
TATGCCAGCTCTTTGGAAAAGGGACCCCCCAAGCCTGGGCCCCCACGACG
CCTTAGTGCCCCCCTGCCCAGTTCCTGTGGAGATCCTGAGAAGCAGCGCC
AAGACAAGATGCGGGAAGAAGGCCTCCAGCTAGTGAGCATGATCCGGGAA
GGGGAAGCCGCAGGTGCCTGTCCAGAGGAGATCTTCTCGGCTCTGCAGTA
CTCGGGCACTGAGGTGCCTCTGCAGTGGTTGCGCTCAGAACTGCCCTACG
TCCTGGAGATGGTGGCTGAGCTGGCTGGACAGCAGGACCCTGGGCTGGGT
GCCTTTTCCTGTCAGGAGGCCCGGAGAGCCTGGCTGGATCGTCATGGCAA
CCTTGATGAAGCTGTGGAGGAGTGTGTGAGGACCAGGCGAAGGAAGGTGC
AGGAGCTCCAGTCTCTAGGCTTTGGGCCTGAGGAGGGGTCTCTCCAGGCA
TTGTTCCAGCACGGAGGTGATGTGTCACGGGCCCTGACTGAGCTACAGCG
CCAACGCCTAGAGCCCTTCCGCCAGCGCCTCTGGGACAGTGGCCCTGAGC
CCACCCCTTCCTGGGATGGGCCAGACAAGCAGAGCCTGGTCAGGCGGCTT
TTGGCAGTCTACGCACTCCCCAGCTGGGGCCGGGCAGAGCTGGCACTGTC
ACTGCTGCAGGAGACACCCAGGAACTATGAGTTGGGGGATGTGGTAGAAG
CTGTGAGGCACAGCCAGGACCGGGCCTTCCTGCGCCGCTTGCTTGCCCAG
GAGTGTGCCGTGTGTGGCTGGGCCCTGCCCCACAACCGGATGCAGGCCCT
GACTTCCTGTGAGTGCACCATCTGTCCTGACTGCTTCCGCCAGCACTTCA
CCATCGCCTTGAAGGAGAAGCACATCACAGACATGGTGTGCCCTGCCTGT
GGCCGCCCCGACCTCACCGATGACACACAGTTGCTCAGCTACTTCTCTAC
CCTTGACATCCAGCTTCGCGAGAGCCTAGAGCCAGATGCCTATGCGTTGT
TCCATAAGAAGCTGACCGAGGGTGTGCTGATGCGGGACCCCAAGTTCTTG
TGGTGTGCCCAGTGCTCCTTTGGCTTCATATATGAGCGTGAGCAGCTGGA
GGCAACTTGTCCCCAGTGTCACCAGACCTTCTGTGTGCGCTGCAAGCGCC
AGTGGGAGGAGCAGCACCGAGGTCGGAGCTGTGAGGACTTCCAGAACTGG
AAACGCATGAACGACCCAGAATACCAGGCCCAGGGCCTAGCAATGTATCT
TCAGGAAAACGGCATTGACTGCCCCAAATGCAAGTTCTCGTACGCCCTGG
CCCGAGGAGGCTGCATGCACTTTCACTGTACCCAGTGCCGCCACCAGTTC
TGCAGCGGCTGCTACAATGCCTTTTACGCCAAGAATAAATGTCCAGAGCC
TAACTGCAGGGTGAAAAAGTCCCTGCACGGCCACCACCCTCGAaACTGCC
TCTTCTACCTGCGGGACTGGACTGCTCTCCGGCTTCAGAAGCTGCTACAG
GACAATAACGTCATGTTTAATACAGAGCCTCCAGCTGGGGCCCGGGCAGT
CCCTGGAGGCGGCTGCCGAGTGATAGAGCAGAAGGAGGTTCCCAATGGGC
TCAGGGACGAAGCTTGTGGCAAGGAAACTCCAGCTGGCTATGCCGGCCTG
TGCCAGGCACACTACAAAGAGTATCTTGTGAGCCTCATCAATGCCCACTC
GCTGGACCCAGCCACCTTGTATGAGGTGGAAGAGCTGGAGACGGCCACTG
AGCGCTACCTGCACGTACGCCCCCAGCCTTTGGCTGGAGAGGATCCCCCT
GCTTACCAGGCCCGCTTGTTACAGAAGCTGACAGAAGAGGTACCCTTGGG
ACAGAGTATCCCCCGCAGGCGGAAGTAGGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSRKGTI
DAAAMPGEEEERAFLVAREELASALRRDSGQAFSLEQLRPLLASSLPLAA
RYLQLDAARLVRCNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWR
GVKFNNPVFRSTVDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEEPDEHQV
ATVTLEVLLLRTELSLLLQNTHPRQQALEQLLEDKVEDDMLQLSEFDPLL
REIAPGPLTTPSVPGSTPGPCFLCGSAPGTLHCPSCKQALCPACDHLFHG
HPSRAHHLRQTLPGVLQGTHLSPSLPASAQPRPQSTSLLALGDSSLSSPN
PASAHLPWHCAACAMLNEPWAVLCVACDRPRGCKGLGLGTEGPQGTGGLE
PDLARGRWACQSCTFENEAAAVLCSICERPRLAQPPSLVVDSRDAGICLQ
PLQQGDALLASAQSQVWYCIHCTFCNSSPGWVCVMCNRTSSPIPAQHAPR
PYASSLEKGPPKPGPPRRLSAPLPSSCGDPEKQRQDKMREEGLQLVSMIR
EGEAAGACPEEIFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGL
GAFSCQEARRAWLDRHGNLDEAVEECVRTRRRKVQELQSLGFGPEEGSLQ
ALFQHGGDVSRALTELQRQRLEPFRQRLWDSGPEPTPSWDGPDKQSLVRR
LLAVYALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLA
QECAVCGWALPHNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPA
CGRPDLTDDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKF
LWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQN
WKRMNDPEYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQ
FCSGCYNAFYAKNKCPEPNCRVKKSLHGHHPRNCLFYLRDWTALRLQKLL
QDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAG
LCQAHYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDP
PAYQARLLQKLTEEVPLGQSIPRRRK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24853
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: KO'd internal Xho1 site at nt2790 (>CTCGAA); *although has another Xho1 site at nt1271*
• Low Temp: No