cDNA Clone Wnk3

• Plasmid: pcDNA FRT TO GST Wnk3 1-862
• Parent Plasmid: pcDNA FRT TO GST
• Expressed: GST-Wnk3 1-862
• Genbank: NM_020922
• Tag: GST

Sequence of Insert

CTCGAGATGGCCACTGATTCAGGCGATCCAGCCAGCACAGAAGATTCTGAGAAACCTGATGGAATTTCATTTGAAAACAGAGTTCCCCAGGTCGCTGCAACTTTGACAGTAGAAGCTAGACTAAAGGAGAAAAACAGTACCTTCTCTGCTTCTGGGGAAACTGTAGAAAGGAAGAGATTTTTCCGAAAGAGTGTTGAAATGACGGAAGATGACAAAGTTGCCGAATCATCCCCCAAAGATGAGAGAATTAAGGCCGCAATGAATATTCCAAGAGTAGATAAGCTTCCTTCAAATGTGTTGAGAGGTGGACAAGAAGTTAAATATGAACAGTGTTCAAAGTCAACCTCAGAAATCTCAAAAGATTGTTTCAAGGAGAAAAATGAAAAGGAAATGGAAGAAGAAGCAGAAATGAAGGCTGTAGCTACTTCTCCTAGTGGCAGATTCCTGAAATTTGACATAGAACTAGGAAGAGGAGCATTTAAAACAGTATATAAAGGACTGGACACTGAAACATGGGTTGAGGTTGCTTGGTGTGAGCTGCAGGACCGAAAGTTAACCAAAGCTGAGCAGCAAAGATTCAAGGAAGAAGCAGAGATGTTGAAGGGTCTCCAGCACCCCAATATAGTTCGATTTTATGATTCCTGGGAATCTATATTAAAAGGAAAGAAATGTATTGTATTAGTGACTGAACTAATGACATCTGGGACCTTAAAGACGTACTTAAAACGATTTAAAGTCATGAAACCAAAGGTCTTAAGGAGCTGGTGCAGGCAAATTTTAAAGGGGTTGCAGTTCTTGCACACTAGGACTCCTCCTATTATTCACCGGGACCTGAAGTGTGACAATATTTTCATCACGGGACCCACTGGATCTGTGAAGATTGGTGATCTAGGATTAGCCACCTTAATGCGTACCTCATTTGCTAAGAGTGTCATTGGAACTCCTGAGTTTATGGCTCCAGAGATGTATGAAGAACACTATGATGAATCCGTAGATGTTTATGCTTTTGGAATGTGTATGCTGGAAATGGCCACATCGGAGTATCCTTATTCTGAGTGTCAGAATGCAGCTCAAATATACCGGAAAGTAACTAGTGGCATAAAACCAGCCAGCTTCAATAAAGTCACTGATCCTGAAGTCAAAGAAATCATTGAAGGATGTATTCGTCAAAACAAATCTGAAAGGTTGTCTATCAGGGACCTATTAAACCATGCATTTTTTGCTGAGGATACAGGACTGAGGGTGGAGTTAGCAGAAGAAGATGATTGCTCAAATTCATCCCTTGCTTTAAGACTCTGGGTTGAAGACCCTAAAAAATTGAAAGGCAAACACAAAGACAATGAAGCTATTGAATTTAGTTTCAACTTAGAAACAGATACACCTGAGGAAGTAGCATATGAAATGGTCAAGTCTGGGTTCTTCCATGAAAGTGATTCCAAAGCTGTTGCTAAATCCATTAGAGACCGGGTGACGCCAATAAAGAAGACAAGAGAGAAGAAGCCTGCTGGCTGTTTGGAAGAACGCAGGGATTCTCAGTGCAAGTCTATGGGGAATGTATTCCCTCAGCCCCAGAATACAACTTTACCCCTTGCTCCCGCTCAGCAAACTGGGGCTGAATGTGAAGAAACTGAAGTTGATCAACATGTTAGACAACAGCTTCTACAAAGAAAACCACAGCAGCACTGCTCCTCTGTTACAGGTGACAATTTGTCTGAGGCAGGAGCTGCATCAGTTATACATTCAGATACTTCAAGTCAGCCCAGTGTAGCCTATTCCTCAAATCAAACGATGGGCTCTCAAATGGTTTCTAATATCCCGCAGGCTGAAGTAAATGTTCCAGGGCAAATTTATTCATCTCAGCAACTAGTAGGACATTACCAGCAAGTTTCAGGGTTACAGAAGCATTCAAAGCTGACTCAGCCGCAGATTTTGCCTTTGGTTCAAGGTCAGTCCACTGTTTTACCTGTACATGTCCTTGGACCGACAGTTGTTTCACAACCCCAGGTTTCCCCATTAACTGTTCAGAAGGTCCCACAGATAAAGCCTGTATCCCAACCAGTTGGAGCTGAACAACAAGCAGCTCTTCTAAAACCAGATTTAGTTCGAAGCTTGAATCAAGATGTGGCAACTACGAAGGAAAACGTCAGTAGCCCTGATAACCCAAGTGGAAATGGCAAACAGGATCGGATCAAACAGAGAAGAGCTTCCTGTCCCCGACCAGAGAAGGGGACTAAATTTCAGCTTACCGTCCTTCAGGTATCAACCTCTGGAGATAACATGGTAGAGTGTCAGCTGGAGACACACAACAACAAGATGGTCACCTTCAAGTTTGATGTTGATGGTGATGCGCCAGAGGATATTGCAGACTATATGGTTGAAGATAACTTTGTGCTGGAAAGTGAGAAAGAAAAATTTGTAGAAGAATTGAGAGCTATTGTAGGTCAAGCCCAGGAGATCCTTCATGTCCACTTTGCCACAGAAAGAGCCACTGGAGTTGATTCTATTACTGTGGACTCCAACAGTAGCCAGACAGGGTCATCTGAACAAGTACAGATAAATTCTACATCTACTCAAACCAGCAATGAATCTGCTTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSPGEFASLEMATDSGDPASTEDSEKPDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSVEMTEDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKSTSEISKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTPIKKTREKKPAGCLEERRDSQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGDNLSEAGAASVIHSDTSSQPSVAYSSNQTMGSQMVSNIPQAEVNVPGQIYSSQQLVGHYQQVSGLQKHSKLTQPQILPLVQGQSTVLPVHVLGPTVVSQPQVSPLTVQKVPQIKPVSQPVGAEQQAALLKPDLVRSLNQDVATTKENVSSPDNPSGNGKQDRIKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDVDGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHVHFATERATGVDSITVDSNSSQTGSSEQVQINSTSTQTSNESA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU47548
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent G18C, T249C and T825C