cDNA Clone cdc34 (yeast)

• Plasmid: pEX CDC34 (yeast) 5FLAG 9HIS
• Parent Plasmid: pEX
• Expressed: Cdc34 (yeast) 5FLAG 9His
• Tag: 6His
• Tag: FLAG
• Species: Yeast

Sequence of Insert

AGATCTATGAGTAGTCGCAAAAGCACCGCTTCTAGCTTACTGTTACGGCAATATAGAGAACTTACTGATCCTAAGAAAGCTATTCCCTCATTTCATATAG
AACTAGAGGATGATTCAAATATTTTCACTTGGAACATAGGTGTTATGGTGCTAAATGAGGATTCCATTTATCATGGAGGGTTTTTCAAAGCTCAAATGAG
ATTTCCGGAAGACTTTCCCTTTTCTCCACCACAGTTTCGATTTACGCCGGCTATCTACCATCCAAACGTTTACAGGGATGGCAGGCTTTGTATTTCTATT
TTACATCAAAGTGGGGATCCTATGACCGACGAACCTGATGCTGAAACGTGGTCCCCCGTGCAGACCGTGGAAAGTGTGTTGATCTCTATAGTATCTCTAT
TAGAGGACCCCAATATCAACTCGCCAGCAAATGTCGATGCCGCTGTTGATTACAGAAAGAACCCTGAACAATACAAGCAAAGAGTCAAAATGGAAGTGGA
AAGATCGAAACAAGATATCCCTAAAGGTTTCATAATGCCCACTTCTGAATCGGCGTACATATCTCAAAGTAAACTAGATGAACCAGAATCGAATAAGGAT
ATGGCGGATAATTTTTGGTATGATAGTGATTTGGACGACGATGAGAACGGGTCCGTCATTTTACAAGACGACGACTACGACGATGGAAACAACCATATCC
CCTTTGAAGATGATGACGTTTACAACTATAACGACAACGACGACGATGACGAAAGAATCGAATTCGAAGATGATGACGATGATGACGACGATAGTATAGA
TAACGACAGCGTCATGGATAGGAAACAACCCCACAAGGCTGAAGATGAAAGTGAAGATGTGGAGGATGTAGAAAGAGTTTCAAAGAAAATACGTACGCTG
CAGGTCGACTCCGGTTCTGCTGCTAGTGGTCATATGGACTACAAGGACGACGATGACAAGGATTACAAAGATGACGACGATAAGCTTATGGACTACAAGG
ACGACGATGACAAGGATTACAAAGATGACGACGATAAGCTTATGGACTACAAGGACGACGATGACAAGCATATGCATCACCACCATCACCATCATCACCA
CTAA

Amino Acid Sequence

MSSRKSTASSLLLRQYRELTDPKKAIPSFHIELEDDSNIFTWNIGVMVLNEDSIYHGGFFKAQMRFPEDFPFSPPQFRFTPAIYHPNVYRDGRLCISILH
QSGDPMTDEPDAETWSPVQTVESVLISIVSLLEDPNINSPANVDAAVDYRKNPEQYKQRVKMEVERSKQDIPKGFIMPTSESAYISQSKLDEPESNKDMA
DNFWYDSDLDDDENGSVILQDDDYDDGNNHIPFEDDDVYNYNDNDDDDERIEFEDDDDDDDDSIDNDSVMDRKQPHKAEDESEDVEDVERVSKKIRTLQV
DSGSAASGHMDYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDKHMHHHHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51269
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: I've sent details in an email to Mark. Please can you make budding yeast Cdc34, with 5FLAG-9His tag at the C-terminus, following the sequence in my email. Thank you!