cDNA Clone DNA mismatch repair protein Msh6 (MSH6)

• Plasmid: pSC-B-MSH6
• Parent Plasmid: pSC-B
• Genbank: NM_000179.2
• Species: Human

Sequence of Insert

ggatccATGTCGCGACAGAGCACCCTGTACAGCTTCTTCCCCAAGTCTCC
GGCGCTGAGTGATGCCAACAAGGCCTCGGCCAGGGCCTCACGCGAAGGCG
GCCGTGCCGCCGCTGCCCCCGGGGCCTCTCCTTCCCCAGGCGGGGATGCG
GCCTGGAGCGAGGCTGGGCCTGGGCCCAGGCCCTTGGCGCGATCCGCGTC
ACCGCCCAAGGCGAAGAACCTCAACGGAGGGCTGCGGAGATCGGTAGCGC
CTGCTGCCCCCACCAGTTGTGACTTCTCACCGGGAGATTTGGTTTGGGCC
AAGATGGAGGGTTACCCCTGGTGGCCTTGTCTGGTTTACAACCACCCCTT
TGATGGAACATTCATCCGCGAGAAAGGGAAATCAGTCCGTGTTCATGTAC
AGTTTTTTGATGACAGCCCAACAAGGGGCTGGGTTAGCAAAAGGCTTTTA
AAGCCATATACAGGTTCAAAATCAAAGGAAGCCCAGAAGGGAGGTCATTT
TTACAGTGCAAAGCCTGAAATACTGAGAGCAATGCAACGTGCAGACGAAG
CCTTAAATAAAGACAAGATTAAGAGGCTTGAATTGGCAGTTTGTGATGAG
CCCTCAGAGCCAGAAGAGGAAGAAGAGATGGAGGTAGGCACAACTTACGT
AACAGATAAGAGTGAAGAAGATAATGAAATTGAGAGTGAAGAGGAAGTAC
AGCCTAAGACACAAGGATCTAGGCGAAGTAGCCGCCAAATAAAAAAACGA
AGGGTCATATCAGATTCTGAGAGTGACATTGGTGGCTCTGATGTGGAATT
TAAGCCAGACACTAAGGAGGAAGGAAGCAGTGATGAAATAAGCAGTGGAG
TGGGGGATAGTGAGAGTGAAGGCCTGAACAGCCCTGTCAAAGTTGCTCGA
AAGCGGAAGAGAATGGTGACTGGAAATGGCTCTCTTAAAAGGAAAAGCTC
TAGGAAGGAAACGCCCTCAGCCACCAAACAAGCAACTAGCATTTCATCAG
AAACCAAGAATACTTTGAGAGCTTTCTCTGCCCCTCAAAATTCTGAATCC
CAAGCCCACGTTAGTGGAGGTGGTGATGACAGTAGTCGCCCTACTGTTTG
GTATCATGAAACTTTAGAATGGCTTAAGGAGGAAAAGAGAAGAGATGAGC
ACAGGAGGAGGCCTGATCACCCCGATTTTGATGCATCTACACTCTATGTG
CCTGAGGATTTCCTCAATTCTTGTACTCCTGGGATGAGGAAGTGGTGGCA
GATTAAGTCTCAGAACTTTGATCTTGTCATCTGTTACAAGGTGGGGAAAT
TTTATGAGCTGTACCACATGGATGCTCTTATTGGAGTCAGTGAACTGGGG
CTGGTATTCATGAAAGGCAACTGGGCCCATTCTGGCTTTCCTGAAATTGC
ATTTGGCCGTTATTCAGATTCCCTGGTGCAGAAGGGCTATAAAGTAGCAC
GAGTGGAACAGACTGAGACTCCAGAAATGATGGAGGCACGATGTAGAAAG
ATGGCACATATATCCAAGTATGATAGAGTGGTGAGGAGGGAGATCTGTAG
GATCATTACCAAGGGTACACAGACTTACAGTGTGCTGGAAGGTGATCCCT
CTGAGAACTACAGTAAGTATCTTCTTAGCCTCAAAGAAAAAGAGGAAGAT
TCTTCTGGCCATACTCGTGCATATGGTGTGTGCTTTGTTGATACTTCACT
GGGAAAGTTTTTCATAGGTCAGTTTTCAGATGATCGCCATTGTTCGAGAT
TTAGGACTCTAGTGGCACACTATCCCCCAGTACAAGTTTTATTTGAAAAA
GGAAATCTCTCAAAGGAAACTAAAACAATTCTAAAGAGTTCATTGTCCTG
TTCTCTTCAGGAAGGTCTGATACCCGGCTCCCAGTTTTGGGATGCATCCA
AAACTTTGAGAACTCTCCTTGAGGAAGAATATTTTAGGGAAAAGCTAAGT
GATGGCATTGGGGTGATGTTACCCCAGGTGCTTAAAGGTATGACTTCAGA
GTCTGATTCCATTGGGTTGACACCAGGAGAGAAAAGTGAATTGGCCCTCT
CTGCTCTAGGTGGTTGTGTCTTCTACCTCAAAAAATGCCTTATTGATCAG
GAGCTTTTATCAATGGCTAATTTTGAAGAATATATTCCCTTGGATTCTGA
CACAGTCAGCACTACAAGATCTGGTGCTATCTTCACCAAAGCCTATCAAC
GAATGGTGCTAGATGCAGTGACATTAAACAACTTGGAGATTTTTCTGAAT
GGAACAAATGGTTCTACTGAAGGAACCCTACTAGAGAGGGTTGATACTTG
CCATACTCCTTTTGGTAAGCGGCTCCTAAAGCAATGGCTTTGTGCCCCAC
TCTGTAACCATTATGCTATTAATGATCGTCTAGATGCCATAGAAGACCTC
ATGGTTGTGCCTGACAAAATCTCCGAAGTTGTAGAGCTTCTAAAGAAGCT
TCCAGATCTTGAGAGGCTACTCAGTAAAATTCATAATGTTGGGTCTCCCC
TGAAGAGTCAGAACCACCCAGACAGCAGGGCTATAATGTATGAAGAAACT
ACATACAGCAAGAAGAAGATTATTGATTTTCTTTCTGCTCTGGAAGGATT
CAAAGTAATGTGTAAAATTATAGGGATCATGGAAGAAGTTGCTGATGGTT
TTAAGTCTAAAATCCTTAAGCAGGTCATCTCTCTGCAGACAAAAAATCCT
GAAGGTCGTTTTCCTGATTTGACTGTAGAATTGAACCGATGGGATACAGC
CTTTGACCATGAAAAGGCTCGAAAGACTGGACTTATTACTCCCAAAGCAG
GCTTTGACTCTGATTATGACCAAGCTCTTGCTGACATAAGAGAAAATGAA
CAGAGCCTCCTGGAATACCTAGAGAAACAGCGCAACAGAATTGGCTGTAG
GACCATAGTCTATTGGGGGATTGGTAGGAACCGTTACCAGCTGGAAATTC
CTGAGAATTTCACCACTCGCAATTTGCCAGAAGAATACGAGTTGAAATCT
ACCAAGAAGGGCTGTAAACGATACTGGACCAAAACTATTGAAAAGAAGTT
GGCTAATCTCATAAATGCTGAAGAACGGAGGGATGTATCATTGAAGGACT
GCATGCGGCGACTGTTCTATAACTTTGATAAAAATTACAAGGACTGGCAG
TCTGCTGTAGAGTGTATCGCAGTGTTGGATGTTTTACTGTGCCTGGCTAA
CTATAGTCGAGGGGGTGATGGTCCTATGTGTCGCCCAGTAATTCTGTTGC
CGGAAGATACCCCCCCCTTCTTAGAGCTTAAAGGATCACGCCATCCTTGC
ATTACGAAGACTTTTTTTGGAGATGATTTTATTCCTAATGACATTCTAAT
AGGCTGTGAGGAAGAGGAGCAGGAAAATGGCAAAGCCTATTGTGTGCTTG
TTACTGGACCAAATATGGGGGGCAAGTCTACGCTTATGAGACAGGCTGGC
TTATTAGCTGTAATGGCCCAGATGGGTTGTTACGTCCCTGCTGAAGTGTG
CAGGCTCACACCAATTGATAGAGTGTTTACTAGACTTGGTGCCTCAGACA
GAATAATGTCAGGTGAAAGTACATTTTTTGTTGAATTAAGTGAAACTGCC
AGCATACTCATGCATGCAACAGCACATTCTCTGGTGCTTGTGGATGAATT
AGGAAGAGGTACTGCAACATTTGATGGGACGGCAATAGCAAATGCAGTTG
TTAAAGAACTTGCTGAGACTATAAAATGTCGTACATTATTTTCAACTCAC
TACCATTCATTAGTAGAAGATTATTCTCAAAATGTTGCTGTGCGCCTAGG
ACATATGGCATGCATGGTAGAAAATGAATGTGAAGACCCCAGCCAGGAGA
CTATTACGTTCCTCTATAAATTCATTAAGGGAGCTTGTCCTAAAAGCTAT
GGCTTTAATGCAGCAAGGCTTGCTAATCTCCCAGAGGAAGTTATTCAAAA
GGGACATAGAAAAGCAAGAGAATTTGAGAAGATGAATCAGTCACTACGAT
TATTTCGGGAAGTTTGCCTGGCTAGTGAAAGGTCAACTGTAGATGCTGAA
GCTGTCCATAAATTGCTGACTTTGATTAAGGAATTATAGgcggccgc

Amino Acid Sequence

MSRQSTLYSFFPKSPALSDANKASARASREGGRAAAAPGASPSPGGDAAW
SEAGPGPRPLARSASPPKAKNLNGGLRRSVAPAAPTSCDFSPGDLVWAKM
EGYPWWPCLVYNHPFDGTFIREKGKSVRVHVQFFDDSPTRGWVSKRLLKP
YTGSKSKEAQKGGHFYSAKPEILRAMQRADEALNKDKIKRLELAVCDEPS
EPEEEEEMEVGTTYVTDKSEEDNEIESEEEVQPKTQGSRRSSRQIKKRRV
ISDSESDIGGSDVEFKPDTKEEGSSDEISSGVGDSESEGLNSPVKVARKR
KRMVTGNGSLKRKSSRKETPSATKQATSISSETKNTLRAFSAPQNSESQA
HVSGGGDDSSRPTVWYHETLEWLKEEKRRDEHRRRPDHPDFDASTLYVPE
DFLNSCTPGMRKWWQIKSQNFDLVICYKVGKFYELYHMDALIGVSELGLV
FMKGNWAHSGFPEIAFGRYSDSLVQKGYKVARVEQTETPEMMEARCRKMA
HISKYDRVVRREICRIITKGTQTYSVLEGDPSENYSKYLLSLKEKEEDSS
GHTRAYGVCFVDTSLGKFFIGQFSDDRHCSRFRTLVAHYPPVQVLFEKGN
LSKETKTILKSSLSCSLQEGLIPGSQFWDASKTLRTLLEEEYFREKLSDG
IGVMLPQVLKGMTSESDSIGLTPGEKSELALSALGGCVFYLKKCLIDQEL
LSMANFEEYIPLDSDTVSTTRSGAIFTKAYQRMVLDAVTLNNLEIFLNGT
NGSTEGTLLERVDTCHTPFGKRLLKQWLCAPLCNHYAINDRLDAIEDLMV
VPDKISEVVELLKKLPDLERLLSKIHNVGSPLKSQNHPDSRAIMYEETTY
SKKKIIDFLSALEGFKVMCKIIGIMEEVADGFKSKILKQVISLQTKNPEG
RFPDLTVELNRWDTAFDHEKARKTGLITPKAGFDSDYDQALADIRENEQS
LLEYLEKQRNRIGCRTIVYWGIGRNRYQLEIPENFTTRNLPEEYELKSTK
KGCKRYWTKTIEKKLANLINAEERRDVSLKDCMRRLFYNFDKNYKDWQSA
VECIAVLDVLLCLANYSRGGDGPMCRPVILLPEDTPPFLELKGSRHPCIT
KTFFGDDFIPNDILIGCEEEEQENGKAYCVLVTGPNMGGKSTLMRQAGLL
AVMAQMGCYVPAEVCRLTPIDRVFTRLGASDRIMSGESTFFVELSETASI
LMHATAHSLVLVDELGRGTATFDGTAIANAVVKELAETIKCRTLFSTHYH
SLVEDYSQNVAVRLGHMACMVENECEDPSQETITFLYKFIKGACPKSYGF
NAARLANLPEEVIQKGHRKAREFEKMNQSLRLFREVCLASERSTVDAEAV
HKLLTLIKEL*

• Vector Type: Mammalian
• Antibiotic: Amp; Kan
• DU Number: DU24879
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: compared to NCBI ref clone has silent mutations at: R62 (CGC>CGA), P92 (CCA>CCG), D180 (GAT>GAC)