cDNA Clone USP47

• Plasmid: pcDNA FRT TO GFP USP47 C177A (N500, D556 restored)
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP-USP47 C177A
• Genbank: NM_001282659.1 → NP_001269588.1 Gene ID 55031
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGCCGCCATGGTGCCCGGCGAGGAGAACCAACTGGTCCCGAAAGAGAT
AGAAAATGCTGCTGAAGAACCTAGAGTCTTATGTATTATACAAGATACTA
CTAATTCAAAGACAGTGAATGAACGGATCACTTTAAATTTACCAGCATCT
ACTCCAGTCAGAAAGCTCTTTGAAGATGTGGCCAACAAAGTAGGCTACAT
AAATGGAACCTTTGACTTGGTGTGGGGAAATGGAATCAATACTGCTGATA
TGGCACCACTGGATCATACCAGTGACAAGTCACTTCTCGACGCTAATTTT
GAGCCAGGAAAGAAGAACTTTCTGCATTTGACAGATAAAGATGGTGAACA
ACCTCAAATACTGCTGGAGGATTCCAGTGCTGGGGAAGACAGTGTTCATG
ACAGGTTTATAGGTCCGCTTCCAAGAGAAGGTTCTGGGGGTTCTACCAGT
GATTATGTCAGCCAAAGCTACTCCTACTCATCTATTTTGAATAAATCAGA
AACTGGATATGTGGGACTAGTAAACCAAGCAATGACTGCCTATTTGAATA
GCCTTTTGCAAACACTTTTTATGACTCCTGAATTTAGGAATGCATTATAT
AAGTGGGAATTTGAAGAATCTGAAGAAGATCCAGTGACAAGTATTCCATA
CCAACTTCAAAGGCTTTTTGTTTTGTTACAAACCAGCAAAAAGAGAGCAA
TTGAAACCACAGATGTTACAAGGAGCTTTGGATGGGATAGTAGTGAGGCT
TGGCAGCAGCATGATGTACAAGAACTATGCAGAGTCATGTTTGATGCTTT
GGAACAGAAATGGAAGCAAACAGAACAGGCTGATCTTATAAATGAGCTAT
ATCAAGGCAAGCTGAAGGACTACGTGAGATGTCTGGAATGTGGTTATGAG
GGCTGGCGAATCGACACATATCTTGATATTCCATTGGTCATCCGACCTTA
TGGGTCCAGCCAAGCATTTGCTAGTGTGGAAGAAGCATTGCATGCATTTA
TTCAGCCAGAGATTCTGGATGGCCCAAATCAGTATTTTTGTGAACGTTGT
AAGAAGAAGTGTGATGCACGGAAGGGCCTTCGGTTTTTGCATTTTCCTTA
TCTGCTGACCTTACAGCTGAAAAGATTCGATTTTGATTATACAACAATGC
ATAGGATTAAACTGAATGATCGAATGACATTTCCCGAGGAACTAGATATG
AGTACTTTTATTGATGTTGAAGATGAGAAATCTCCTCAGACTGAAAGTTG
CACTGACAGTGGAGCAGAAAATGAAGGTAGTTGTCACAGTGATCAGATGA
GCAACGATTTCTCCAATGATGATGGTGTTGATGAAGGAATCTGTCTTGAA
ACCAATAGTGGAACTGAAAAGATCTCAAAATCTGGACTTGAAAAGAATTC
CTTGATCTATGAACTTTTCTCTGTTATGGCTCATTCTGGGAGCGCTGCTG
GTGGTCATTATTATGCATGTATAAAGTCATTCAGTGATGAGCAGTGGTAC
AGCTTCAATGATCAACATGTCAGCAGGATAACACAAGAGGACATTAAGAA
AACACATGGTGGATCTTCAGGAAGCAGAGGATATTATTCTAGTGCTTTCG
CAAGTTCCACAAATGCATATATGCTGATCTATAGACTGAAGGATCCAGCC
AGAAATGCAAAATTTCTAGAAGTGGATGAATACCCAGAACATATTAAAAA
CTTGGTGCAGAAAGAGAGAGAGTTGGAAGAACAAGAAAAGAGACAACGAG
AAATTGAGCGCAATACATGCAAGATAAAATTATTCTGTTTGCATCCTACA
AAACAAGTAATGATGGAAAATAAATTGGAGGTTCATAAGGATAAGACATT
AAAGGAAGCAGTAGAAATGGCTTATAAGATGATGGATTTAGAAGAGGTAA
TACCCCTGGATTGCTGTCGCCTTGTTAAATATGATGAGTTTCATGATTAT
CTAGAACGGTCATATGAAGGAGAAGAAGATACACCAATGGGGCTTCTACT
AGGTGGCGTCAAGTCAACATATATGTTTGATCTGCTGTTGGAGACGAGAA
AGCCTGATCAGGTTTTCCAATCTTATAAACCTGGAGAAGTGATGGTGAAA
GTTCATGTTGTTGATCTAAAGGCAGAATCTGTAGCTGCTCCTATAACTGT
TCGTGCTTACTTAAATCAGACAGTTACAGAATTCAAACAACTGATTTCAA
AGGCCATCCATTTACCTGCTGAAACAATGAGAATAGTGCTGGAACGCTGC
TACAATGATTTGCGTCTTCTCAGTGTCTCCAGTAAAACCCTGAAAGCTGA
AGGATTTTTTAGAAGTAACAAGGTGTTTGTTGAAAGCTCCGAGACTTTGG
ATTACCAGATGGCCTTTGCAGACTCTCATTTATGGAAACTCCTGGATCGG
CATGCAAATACAATCAGATTATTTGTTTTGCTACCTGAACAATCCCCAGT
ATCTTATTCCAAAAGGACAGCATACCAGAAAGCTGGAGGCGATTCTGGTA
ATGTGGATGATGACTGTGAAAGAGTCAAAGGACCTGTAGGAAGCCTAAAG
TCTGTGGAAGCTATTCTAGAAGAAAGCACTGAAAAACTCAAAAGCTTGTC
ACTGCAGCAACAGCAGGATGGAGATAATGGGGACAGCAGCAAAAGTACTG
AGACAAGTGACTTTGAAAACATCGAATCACCTCTCAATGAGAGGGACTCT
TCAGCATCAGTGGATAATAGAGAACTTGAACAGCATATTCAGACTTCTGA
TCCAGAAAATTTTCAGTCTGAAGAACGATCAGACTCAGATGTGAATAATG
ACAGGAGTACAAGTTCAGTGGACAGTGATATTCTTAGCTCCAGTCATAGC
AGTGATACTTTGTGCAATGCAGACAATGCTCAGATCCCTTTGGCTAATGG
ACTTGACTCTCACAGTATCACAAGTAGTAGAAGAACGAAAGCAAATGAAG
GGAAAAAAGAAACATGGGATACAGCAGAAGAAGACTCTGGAACTGATAGT
GAATATGATGAGAGTGGCAAGAGTAGGGGAGAAATGCAGTACATGTATTT
CAAAGCTGAACCTTATGCTGCAGATGAAGGTTCTGGGGAAGGACATAAAT
GGTTGATGGTGCATGTTGATAAAAGAATTACTCTGGCAGCTTTCAAACAA
CATTTAGAGCCCTTTGTTGGAGTTTTGTCCTCTCACTTCAAGGTCTTTCG
AGTGTATGCCAGCAATCAAGAGTTTGAGAGCGTCCGGCTGAATGAGACAC
TTTCATCATTTTCTGATGACAATAAGATTACAATTAGACTGGGGAGAGCA
CTTAAAAAAGGAGAATACAGAGTTAAAGTATACCAGCTTTTGGTCAATGA
ACAAGAGCCATGCAAGTTTCTGCTAGATGCTGTGTTTGCTAAAGGAATGA
CTGTACGGCAATCAAAAGAGGAATTAATTCCTCAGCTCAGGGAGCAATGT
GGTTTAGAGCTCAGTATTGACAGGTTTCGTCTAAGGAAAAAAACATGGAA
GAATCCTGGCACTGTCTTTTTGGATTATCATATTTATGAAGAAGATATTA
ATATTTCCAGCAACTGGGAGGTTTTCCTTGAAGTTCTTGATGGGGTAGAG
AAGATGAAGTCCATGTCACAGCTTGCAGTTTTGTCAAGACGGTGGAAGCC
TTCAGAGATGAAGTTGGATCCCTTCCAGGAGGTTGTATTGGAAAGCAGTA
GTGTGGACGAATTGCGAGAGAAGCTTAGTGAAATCAGTGGGATTCCTTTG
GATGATATTGAATTTGCTAAGGGTAGAGGAACATTTCCCTGTGATATTTC
TGTCCTTGATATTCATCAAGATTTAGACTGGAATCCTAAAGTTTCTACGC
TGAATGTCTGGCCTCTTTATATCTGTGATGATGGTGCGGTCATATTTTAT
AGGGATAAAACAGAAGAATTAATGGAACTGACAGATGAGCAAAGAAATGA
ACTGATGAAAAAAGAAAGCAGTCGACTCCAGAAGACTGGACATCGTGTAA
CATACTCACCTCGTAAAGAGAAAGCACTAAAAATATATCTGGATGGAGCA
CCAAATAAAGATCTGACTCAAGACTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFAS
LEKLAAAMVPGEENQLVPKEIENAAEEPRVLCIIQDTTNSKTVNERITLN
LPASTPVRKLFEDVANKVGYINGTFDLVWGNGINTADMAPLDHTSDKSLL
DANFEPGKKNFLHLTDKDGEQPQILLEDSSAGEDSVHDRFIGPLPREGSG
GSTSDYVSQSYSYSSILNKSETGYVGLVNQAMTAYLNSLLQTLFMTPEFR
NALYKWEFEESEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWD
SSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLE
CGYEGWRIDTYLDIPLVIRPYGSSQAFASVEEALHAFIQPEILDGPNQYF
CERCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPE
ELDMSTFIDVEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSNDDGVDEG
ICLETNSGTEKISKSGLEKNSLIYELFSVMAHSGSAAGGHYYACIKSFSD
EQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRL
KDPARNAKFLEVDEYPEHIKNLVQKERELEEQEKRQREIERNTCKIKLFC
LHPTKQVMMENKLEVHKDKTLKEAVEMAYKMMDLEEVIPLDCCRLVKYDE
FHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLETRKPDQVFQSYKPGE
VMVKVHVVDLKAESVAAPITVRAYLNQTVTEFKQLISKAIHLPAETMRIV
LERCYNDLRLLSVSSKTLKAEGFFRSNKVFVESSETLDYQMAFADSHLWK
LLDRHANTIRLFVLLPEQSPVSYSKRTAYQKAGGDSGNVDDDCERVKGPV
GSLKSVEAILEESTEKLKSLSLQQQQDGDNGDSSKSTETSDFENIESPLN
ERDSSASVDNRELEQHIQTSDPENFQSEERSDSDVNNDRSTSSVDSDILS
SSHSSDTLCNADNAQIPLANGLDSHSITSSRRTKANEGKKETWDTAEEDS
GTDSEYDESGKSRGEMQYMYFKAEPYAADEGSGEGHKWLMVHVDKRITLA
AFKQHLEPFVGVLSSHFKVFRVYASNQEFESVRLNETLSSFSDDNKITIR
LGRALKKGEYRVKVYQLLVNEQEPCKFLLDAVFAKGMTVRQSKEELIPQL
REQCGLELSIDRFRLRKKTWKNPGTVFLDYHIYEEDINISSNWEVFLEVL
DGVEKMKSMSQLAVLSRRWKPSEMKLDPFQEVVLESSSVDELREKLSEIS
GIPLDDIEFAKGRGTFPCDISVLDIHQDLDWNPKVSTLNVWPLYICDDGA
VIFYRDKTEELMELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIY
LDGAPNKDLTQD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24926
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: intermediate to correcting no-silent mutations in C177A mutant background. isoform 4 (also known as isoform a). silent changes at T379, Q1270, T1280 and L1307 and non-silent mutation still at V474>A.; NCBI updated changes G814>V, Y1029>H are confirmed in this construct.