cDNA Clone Ubiquitin

• Plasmid: pET28a 6His-Halo.TEV-ubiquitin dimer
• Parent Plasmid: pET28a 6His-Halo.TEV
• Expressed: 6His-HALO-ubiquitin dimer
• Genbank: P62988
• Tag: 6His
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCGCCGGCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCAT
CACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGA
TCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCT
GGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAA
AGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGCGGCATGCAGATCT
TCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGT
GACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCC
TCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGAC
GCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTC
CTCCGTCTCAGAGGCGGCTGATAAGCGGCCGC

Amino Acid Sequence

MHHHHHHMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPT
SSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIE
ALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPE
FARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPF
LNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTP
GVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTL
EISGENLYFQGGSAGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEG
IPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLT
GKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDY
NIQKESTLHLVLRLRGG**

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU24951
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 45630