cDNA Clone HRS FYVE

• Plasmid: pGEX6P1 HRS FYVE 147-223 (H176A/H177A)
• Parent Plasmid: pGEX6P1
• Expressed: GST-HRS FYVE 147-223 (H176A/H177A)
• Tag: GST

Sequence of Insert

CTCGAGGAAAGTGATGCCATGTTCGCTGCTGAAAGAGCCCCTGACTGGGTGGATGCTGAGGAATGCCATCGGTGCAGAGTACAGTTTGGGGTGGTGACCCGCAAGCATCACTGCCGAGCATGTGGGCAGATCTTCTGTGGCAAGTGCTCCTCCAAGTACTCCACCATCCCCAAGTTCGGCATTGAGAAGGAGGTGCGCGTGTGTGAGCCCTGCTATGAGCAGCTGAACAAGAAGGCATGAGAATTCTGA

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFPGRLEESDAMFAAERAPDWVDAEECHRCRVQFGVVTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQLNKKA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU47535
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please utilise DU45781 and insert H176A/H177A mutations into each FYVE domain and forward for protein production in E.coli. Thanks. --> SDM looped out 2nd FYVE domain, will amplify up this one w/o stop and subclone back into Xho site