cDNA Clone DENND2C

• Plasmid: pSC-B DENND2C
• Parent Plasmid: pSC-B
• Genbank: NM_001256404.1
• Species: Human

Sequence of Insert

GGATCCATGGATGTTGGTTTTTCTCGTACTACTGTTCAGACACTGTCAAGAAGCCACTGCAAAAACATCAAACAAAAAATTTCTCAATGGGAAGGAAGGGCTAATGGTATATCTAATCCAGAAAAGTGGTGTCCAAAGGACTTTGGAGTGAGATATAACTGTCACCAAGAGATCCGTCTTAAGAAAAATCCTATAGCTGAGAGAAAGAGCAAAAACTTGGATGTAACCAGCCGTGAAAATGTGGGTCTAGATATAAATGAAAATACCAAAAGCCATGATCAAAGTGAGAATGAAAATAAGAAACATGAATATGACGATACACACTTCTTTAAAAATGAATCAGAATCCAACTGGGTATGTTCTCGGGTCAAAGAAATTGAAAGCTGTAAAGAAGATGTCTTAGATCCAGAGACTTCATTACCTCCAGGAAACTTCTATACCTCACAAATACTGTGGAAGAAAATAGAAGCACTTCCCCCAGATAAACTCTTAAATTTGGCTTTAGAACATTGTGACTCTTCAGAAAAAGAACTGAACTTCAGAGTTCTGGATAGTTCATACGGAATAACCAAGAGCTTAGAAAATATTTACTCTGAACCTGAGGGGCAAGAATGTGGACCTTCCATAAATCCTTTGCCAAAACCTCGTAGGACATTCAGATATTTATCCGAATCTGGTGTTACGCCGTATAAAGAAAGAAACTGTGACAAAAAATACTGTGAAAATAACTCTTGTGCACAATCTTCTTTGGCCTCTTCTCAGGAACCTGAACCAAAGAAATATGGTGGAAAAATCAGAGGAAGATCTAAAAGGAAATCCTTTGAATTTGAGGATATTCAGCACTTTCGAAATCGGAACTCACAGACGATTCGTGAAGAACTTGGAAGAAATTCTGGGTCAGCACTTTATTACACACAGTCTGAGGACAATATCTATGAAGATATCATATATCCCACCAAAGAAAATCCATATGAAGATATTCCAGTGCAGCCTTTACCTATGTGGAGATCCCCTTCAGCATGGAAGCTACCACCCGCTAAAAGTGCTTTTAAAGCACCCAAGCTCCCTCCAAAACCTCAGTTCCTTCACCGGAAGACTATGGAAGTAAAGAACTCACAGGCTTATTTGCGGTCAAAGCTTACAAAAGATACAACTTTGCCGGTCACTTTAACGGAATGGAAGCTTTTCCGAGCTGGTGAAGTTGCAAACACGAAAAGGAAAAATCTTCCAAGGCTTGTATTGAAAATAGATGACATATTTGAATCTAAAAGAGGGAAGAAGAAGGTAAAGTTACATTCTTACACTGGAAAGGAATTACCTCCGACAAAAGGTGAAACCAGTGGGAATGAAAGTGATGCCGAGTATCTGCCAAAGAATCGCCATAAACGCTTAGCACAACTGCAACCGTCTTCCAAGAGGAATCCTCACTACCAGACCTTGGAGCGGGATCTTATTGAATTACAGGAGCAGCAGCTGTTTGAACTTTTTGTGGTGGTGTCTCTACAGAAGAAACCATCAGGAATAAGCTATATTCCCCAGGTCATACAACAATTCCCTGGCAAGGATGATCATGGCTATAAGCAGTCCAAAGACATGGAAGAGAGACTTAAAGTTATTCCAAAATTTTGTTTTCCTGATTCAAAGGACTGGATGCCAACCTCAGAACTCAAGAGTGAAACATTCTCCTTTGTCTTGACTGGTGAAGATGGAAGCCGGTGGTTTGGTTACTGTAAGAAGCTCTTGCCAGTAGGCAAAGGAAAGCGACTCCCTGAGGTATACTGCATGGTTAGTCGCCTAGGCTGCTTCAATCTTTTTTCAAAGATTCTGGATGAAGTAGAGAAGAGAAGAGAAATGTCTCCAGCCCTTGTTTACCCATTCATGCGAAGTGTCATGGAAGCTCCTTTCCCAGCTCCTGGACGCACCATCACAGTTAAGAGTTACCTCCCTGGGGCTGGAGATGAGTCCATTGAACTCTGCCGACCACTAGATTCCCGATTGGAACATGTTGATTTTAAATGTCTCTTTAAGTGCCTGAGTGTGTGTCATCTCATCCGGGTCTGTGCCTCTCTCCTTTTGGAGCGTAGGGTAATCTTTGTTGCCAACAGCCTAAGCACCCTGTCAAAATGTGGCCATGCTGTGGTAGCTACACTGTATCCGTTCACCTGGCAGCATACCTATATCCCAGTCCTGCCAGCATCTATGATTGACATCGTGTGCTCACCTACACCATTCCTTATTGGAATCCTGTCTTGCTCCTTACCACAGCTCCAGGACCTACCCATTGAAGAGGTGCTGATAGTTGATCTCTGTGCAGACAAGTTCTTACAGGAGGTATCTGATGAGGATGAAATTCTACCACCAAAACTTCAAGCTGCCCTGATGCAGATTTTGGAAGAACGAAATGAAATCTTGACTCAGGAGCAGAATTTTTCACAAGATGTGACACTCAACTCTCTGGTGTCCGAAGCATTTGTCAGGTTTTTTGTGGAGTTGGTAGGACATTATTCTTTGAACATGACTGTCACTGAGCGTGGGGAGCGTGTTTTCCAAAGGGAACCATTCCGTAAGTCCCACACCTCCCGAAGTGTACGCCACTTCCTGGATCTCTTCATGGAAACTCAGATGTTTGCAGGATTCATCCAGGACCGAGAGCTTCGGAAAAGTGGAGTTAAAGGTTTGTTTGAGATCCGGGCCATCCAGTATTTGGAAACAATTCCTGAGTCGGAGCCCAGTGGGATGAATAGAATTTTGCGGAGTCTTGGAAGCAAAATGAAATTTCTGCAAAAGAAATGAGCGGCCGC

Amino Acid Sequence

MDVGFSRTTVQTLSRSHCKNIKQKISQWEGRANGISNPEKWCPKDFGVRYNCHQEIRLKKNPIAERKSKNLDVTSRENVGLDINENTKSHDQSENENKKHEYDDTHFFKNESESNWVCSRVKEIESCKEDVLDPETSLPPGNFYTSQILWKKIEALPPDKLLNLALEHCDSSEKELNFRVLDSSYGITKSLENIYSEPEGQECGPSINPLPKPRRTFRYLSESGVTPYKERNCDKKYCENNSCAQSSLASSQEPEPKKYGGKIRGRSKRKSFEFEDIQHFRNRNSQTIREELGRNSGSALYYTQSEDNIYEDIIYPTKENPYEDIPVQPLPMWRSPSAWKLPPAKSAFKAPKLPPKPQFLHRKTMEVKNSQAYLRSKLTKDTTLPVTLTEWKLFRAGEVANTKRKNLPRLVLKIDDIFESKRGKKKVKLHSYTGKELPPTKGETSGNESDAEYLPKNRHKRLAQLQPSSKRNPHYQTLERDLIELQEQQLFELFVVVSLQKKPSGISYIPQVIQQFPGKDDHGYKQSKDMEERLKVIPKFCFPDSKDWMPTSELKSETFSFVLTGEDGSRWFGYCKKLLPVGKGKRLPEVYCMVSRLGCFNLFSKILDEVEKRREMSPALVYPFMRSVMEAPFPAPGRTITVKSYLPGAGDESIELCRPLDSRLEHVDFKCLFKCLSVCHLIRVCASLLLERRVIFVANSLSTLSKCGHAVVATLYPFTWQHTYIPVLPASMIDIVCSPTPFLIGILSCSLPQLQDLPIEEVLIVDLCADKFLQEVSDEDEILPPKLQAALMQILEERNEILTQEQNFSQDVTLNSLVSEAFVRFFVELVGHYSLNMTVTERGERVFQREPFRKSHTSRSVRHFLDLFMETQMFAGFIQDRELRKSGVKGLFEIRAIQYLETIPESEPSGMNRILRSLGSKMKFLQKK*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU26334
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 300bp missing from EST clone. Gibson used to insert this. silent mutation N447