cDNA Clone USP24

• Plasmid: pcDNA5-FRT/TO-GFP USP24 V2308A
• Parent Plasmid: pcDNA5-FRT/TO-GFP
• Expressed: GFP-USP24 V2308A
• Genbank: Gene 23358 NP_056121.2 NM_015306.2
• Tag: GFP
• Species: Human

Sequence of Insert

gcggccgctATGACCACGCTGCTGTGCATGGGCTTCTCAGACCCCGCCACCATCCGCAAGGCCCTGCGCCTGGCCAAGAACGACATTAACGAGGCCGTGG
CACTGCTCACCAACGAGCGGCCGGGCCTCGACTACGGCGGCTACGAGCCCATGGACAGCGGCGGTCTTTCCGAGTCTGATGAGAATTGTAGAAGGTTTAT
GGACAGGTGTATGCCTGAAGCATTTAAAAAGCTCCTGACATCAAGTGCTGTTCACAAGTGGGGTACTGAAATTCATGAAGGAATTTACAACATGTTGATG
CTATTAATAGAACTGGTCGCAGAGAGAATAAAACAAGATCCAATTCCCACTGGTCTCCTGGGTGTGCTTACAATGGCTTTCAATCCTGATAATGAATACC
ATTTTAAAAACAGAATGAAAGTGTCTCAAAGGAATTGGGCAGAAGTGTTTGGAGAGGGAAATATGTTTGCTGTTTCACCTGTATCGACTTTCCAAAAGGA
GCCTCATGGATGGGTTGTGGATTTGGTAAATAAGTTTGGAGAATTAGGTGGATTTGCAGCAATCCAAGCCAAGCTCCATTCAGAAGATATAGAACTTGGG
GCTGTCTCAGCACTGATTCAGCCCTTAGGAGTGTGTGCAGAGTACCTCAATTCCTCCGTGGTACAGCCCATGCTAGACCCAGTCATTCTTACTACAATCC
AGGATGTACGGAGTGTAGAAGAGAAAGACCTCAAAGACAAGAGATTGGTTAGCATCCCTGAGCTCTTGTCTGCCGTTAAGTTACTTTGCATGCGCTTCCA
ACCGGATCTGGTGACAATTGTGGATGACCTTCGACTAGATATTCTATTGCGCATGCTGAAATCACCACATTTCAGTGCTAAGATGAATTCTCTCAAAGAA
GTAACCAAACTAATAGAAGATAGCACTTTATCCAAATCTGTGAAGAATGCTATAGATACAGACAGATTATTAGATTGGCTAGTTGAAAACTCAGTTCTGT
CGATTGCACTGGAAGGCAACATAGACCAAGCACAATACTGTGACCGTATAAAGGGAATTATTGAACTCTTGGGTAGTAAATTGTCGTTAGATGAACTCAC
TAAAATTTGGAAGATACAGTCAGGACAATCATCTACTGTGATTGAGAACATTCATACTATTATTGCTGCAGCGGCTGTGAAATTTAATTCAGATCAGCTT
AATCATTTGTTTGTTCTCATTCAGAAGGTTTTAGACGTACTCTGGGAACTGGCTCACCTTCCAACCCTGCCCAGTAGCCTTATTCAGCAGGCCTTGGAGG
AGCACCTGACAATCCTTAGTGATGCATATGCAGTGAAAGAAGCAATCAAGAGGAGCTACATCATCAAGTGCATAGAAGATATTAAGAGGTCATCTCAGCT
TAATAATCCCCAGTTTGTATGGGTGGTACCAGCTTTGCGTCAGCTCCATGAAATTACTCGCTCATTCATAAAACAAACCTATCAAAAGCAAGACAAGAGC
ATTATTCAAGACTTGAAGAAGAATTTTGAAATAGTGAAATTGGTAACGGGAAGTTTGATCGCTTGTCATCGGCTTGCAGCTGCTGTGGCCGGGCCTGGAG
GCTTAAGTGGCTCGACACTAGTGGATGGCCGGTACACTTACCGGGAGTATTTAGAGGCACATCTAAAATTTCTAGCGTTTTTCTTGCAAGAAGCTACTCT
GTATCTGGGCTGGAATCGTGCCAAGGAGATCTGGGAGTGTCTTGTAACTGGCCAGGATGTTTGTGAATTAGATAGAGAGATGTGTTTTGAATGGTTTACA
AAAGGACAGCATGATCTTGAGAGTGATGTTCAGCAGCAGCTCTTCAAGGAGAAAATTCTTAAATTGGAGTCATATGAAATCACTATGAATGGTTTTAACT
TATTTAAAACTTTTTTTGAAAATGTGAATCTTTGTGATCATCGATTGAAAAGACAAGGAGCTCAGTTGTATGTAGAAAAGCTGGAATTGATAGGAATGGA
TTTCATTTGGAAAATAGCCATGGAATCACCTGATGAAGAAATTGCTAATGAAGCTATTCAGCTAATCATAAACTATAGTTACATTAATCTAAATCCTAGA
TTAAAGAAGGATTCAGTATCTTTACATAAGAAATTCATTGCTGATTGCTACACAAGATTAGAAGCAGCCAGTTCAGCACTTGGTGGCCCCACTCTAACAC
ATGCTGTGACCAGAGCAACAAAAATGCTTACAGCAACTGCCATGCCAACTGTAGCAACCTCAGTTCAGTCTCCTTATAGATCTACTAAACTTGTAATAAT
TGAGAGATTGCTGCTTCTGGCAGAGCGCTATGTGATCACTATAGAGGATTTTTACTCTGTTCCACGAACTATTCTACCTCATGGTGCCTCATTTCATGGA
CATCTTTTAACCCTTAATGTTACCTATGAGTCTACCAAAGATACCTTCACTGTCGAGGCTCACAGTAATGAAACCATAGGGAGTGTCCGGTGGAAAATAG
CCAAGCAGTTGTGCTCTCCTGTGGATAATATACAGATATTTACAAATGATAGCCTGCTGACAGTGAATAAAGATCAAAAGCTACTCCACCAACTGGGCTT
TTCTGATGAACAAATCCTTACAGTGAAGACTTCTGGCAGTGGGACCCCATCTGGGAGTTCAGCAGATTCTTCAACCAGCTCCAGCAGCAGCAGCAGTGGG
GTTTTTAGTTCTTCATATGCCATGGAGCAGGAGAAATCCCTCCCTGGTGTAGTGATGGCTCTCGTATGTAACGTATTTGACATGCTTTATCAGCTCGCCA
ATCTGGAAGAGCCAAGGATAACTCTACGAGTACGGAAGCTTCTGCTCTTGATACCCACTGATCCAGCCATTCAGGAAGCCCTTGATCAACTTGATTCTTT
AGGAAGAAAGAAAACATTGCTGTCTGAATCAAGTTCTCAGTCCTCAAAATCTCCATCCCTGTCATCAAAGCAACAGCACCAGCCAAGTGCCAGTTCAATT
TTAGAAAGTCTGTTTCGATCTTTTGCCCCGGGAATGTCTACCTTCAGAGTGCTCTACAACTTAGAAGTTCTAAGCTCCAAACTCATGCCAACAGCTGATG
ATGACATGGCCAGAAGCTGTGCCAAATCCTTCTGTGAAAACTTCCTCAAAGCTGGCGGTTTGAGTTTGGTTGTAAATGTCATGCAGAGAGACTCCATCCC
ATCAGAAGTAGACTATGAAACAAGGCAGGGTGTTTATTCCATCTGTCTACAGCTTGCAAGATTTTTACTTGTCGGACAAACAATGCCCACGTTATTAGAT
GAAGACCTCACCAAAGATGGTATAGAAGCACTTTCTTCCCGCCCATTCCGAAATGTCAGCCGGCAGACAAGCAGACAGATGTCCTTATGTGGTACCCCAG
AAAAGTCATCCTACCGACAGTTGTCCGTGTCTGATAGGTCTTCTATTAGGGTTGAGGAAATCATCCCTGCTGCTCGAGTTGCAATACAAACAATGGAAGT
AAGTGATTTCACTTCTACTGTGGCTTGCTTCATGAGATTGTCATGGGCTGCGGCTGCAGGACGGCTTGATCTTGTTGGGAGTAGCCAGCCAATTAAAGAA
AGTAATTCCCTGTGTCCTGCTGGAATTCGAAACAGACTCAGCAGTTCAGGAAGCAATTGCAGCTCTGGAAGTGAAGGAGAACCAGTAGCCCTGCATGCGG
GAATCTGTGTTCGACAACAGTCTGTATCCACCAAAGACTCGCTGATTGCGGGAGAGGCTTTGTCTCTTCTTGTTACGTGCCTACAGCTTCGGAGCCAGCA
ACTGGCATCTTTCTATAACTTGCCCTGTGTTGCTGATTTCATCATTGATATTCTGCTCGGATCACCAAGTGCTGAGATTCGCCGGGTTGCCTGTGATCAG
CTGTACACTCTTAGTCAGACAGACACATCAGCGCATCCAGATGTGCAGAAGCCAAATCAGTTTCTTCTAGGCGTAATCCTCACGGCTCAGCTGCCTCTCT
GGTCTCCAACTAGTATTATGAGAGGAGTCAATCAGAGACTGTTATCTCAGTGTATGGAGTATTTTGATTTGAGATGCCAGTTATTAGATGATCTGACAAC
TTCAGAAATGGAGCAGTTAAGGATCAGCCCAGCTACGATGCTTGAAGATGAGATTACTTGGCTGGATAACTTTGAACCTAATCGTACAGCTGAATGTGAG
ACCAGTGAAGCGGACAACATCTTACTGGCAGGGCACTTACGCCTCATCAAGACCCTTCTTTCACTCTGTGGGGCAGAAAAGGAAATGCTTGGTTCATCAC
TCATTAAACCATTGTTAGATGACTTCCTTTTCCGAGCTTCTAGAATTATTTTAAATAGTCATTCTCCAGCTGGCAGTGCCGCCATCAGTCAACAGGACTT
TCATCCAAAGTGTAGTACAGCGAATAGCCGATTGGCAGCCTATGAAGTCCTTGTGATGTTGGCTGATAGTTCACCTTCAAATCTTCAAATTATTATAAAA
GAACTGCTTTCTATGCATCACCAGCCTGACCCTGCTCTTACCAAGGAGTTTGATTACCTTCCCCCAGTGGATAGCAGGTCCAGTTCAGGGTTTGTGGGGC
TGAGAAATGGTGGTGCAACTTGTTATATGAATGCAGTCTTCCAGCAGCTGTATATGCAACCTGGGCTCCCTGAGTCATTACTTTCAGTGGATGATGACAC
AGACAATCCAGATGATAGCGTGTTTTACCAAGTGCAGTCTCTCTTTGGACATTTAATGGAAAGCAAGCTGCAGTACTATGTACCTGAGAATTTTTGGAAG
ATTTTCAAGATGTGGAATAAAGAACTTTATGTGAGAGAACAGCAGGATGCATATGAATTCTTTACTAGTCTCATTGATCAGATGGATGAATACCTCAAGA
AAATGGGGAGAGACCAAATTTTTAAGAATACATTTCAGGGCATCTACTCTGATCAGAAGATCTGTAAAGACTGTCCTCACAGATATGAGCGTGAAGAAGC
TTTCATGGCTCTCAATCTAGGAGTGACTTCTTGTCAGAGTTTGGAAATTTCTTTGGACCAATTTGTTAGAGGAGAAGTTCTAGAAGGAAGTAATGCGTAC
TACTGTGAAAAGTGTAAAGAAAAGAGAATAACAGTGAAAAGGACCTGTATTAAATCTTTACCTAGCGTCTTGGTAATTCACCTAATGAGATTTGGGTTTG
ACTGGGAAAGCGGACGCTCCATTAAATATGATGAACAAATAAGGTTTCCCTGGATGCTAAACATGGAGCCTTACACAGTTTCAGGAATGGCTCGCCAAGA
TTCTTCTTCTGAAGTTGGGGAAAATGGGCGAAGTGTGGATCAGGGCGGTGGAGGATCCCCACGAAAAAAGGTTGCCCTCACAGAAAACTATGAACTTGTC
GGTGTCATCGTACACAGTGGGCAGGCACACGCAGGCCACTACTATTCCTTCATTAAGGACAGGCGAGGGTGTGGAAAAGGAAAGTGGTATAAATTTAATG
ACACAGTTATAGAAGAATTTGACCTAAATGACGAGACCCTGGAGTATGAATGCTTTGGAGGAGAATATAGACCAAAAGTTTATGATCAAACAAACCCATA
CACTGATGTGCGCCGAAGATACTGGAATGCCTATATGCTTTTCTACCAAAGGGTGTCTGATCAGAACTCCCCAGTATTACCAAAGAAAAGTCGAGTCAGC
GTTGTACGGCAGGAAGCTGAGGATCTCTCTCTGTCAGCTCCATCTTCACCAGAAATTTCACCTCAGTCATCCCCTCGGCCCCATAGGCCGAACAATGACC
GGCTGTCTATTCTTACCAAGCTGGTTAAAAAAGGCGAGAAGAAAGGACTGTTTGTGGAGAAAATGCCTGCTCGAATATACCAGATGGTGAGAGATGAGAA
CCTCAAGTTTATGAAGAATAGAGATGTATACAGTAGTGATTATTTCAGTTTTGTTTTGTCTTTAGCTTCATTGAATGCTACTAAATTAAAGCATCCATAT
TATCCTTGCATGGCAAAGGTGAGCTTACAGCTTGCTATTCAATTCCTTTTTCAAACTTATCTACGGACAAAGAAGAAACTCAGGGTTGATACTGAAGAAT
GGATTGCTACCATTGAAGCATTGCTTTCAAAAAGTTTTGATGCTTGTCAGTGGTTAGTTGAATATTTTATTAGTTCTGAAGGACGAGAATTGATAAAGAT
TTTCTTACTGGAGTGCAATGTGAGAGAAGTACGAGTTGCTGTGGCCACCATTCTGGAGAAAACCCTAGACAGTGCCTTGTTTTATCAGGATAAGTTAAAA
AGCCTTCATCAGTTACTGGAGGTACTACTTGCTCTGTTGGACAAAGACGTCCCAGAAAATTGTAAAAACTGTGCTCAGTACTTTTTCCTGTTCAACACTT
TTGTACAAAAGCAAGGAATTAGGGCTGGAGATCTTCTTCTGAGGCATTCAGCTCTGCGGCACATGATCAGCTTCCTCCTAGGGGCCAGTCGGCAAAACAA
TCAGATACGTCGATGGAGTTCAGCACAAGCACGAGAATTTGGGAATCTTCACAATACAGTGGCGTTACTTGTTTTGCATTCAGATGTCTCATCCCAAAGG
AATGTTGCTCCTGGCATATTTAAGCAACGACCACCCATTAGCATTGCTCCCTCAAGCCCTCTGTTGCCCCTCCATGAGGAGGTAGAAGCCTTGTTGTTCA
TGTCTGAAGGGAAACCTTACCTGTTAGAGGTAATGTTTGCTTTGCGGGAGCTGACAGGCTCGCTCTTGGCACTCATTGAGATGGTAGTGTACTGCTGTTT
CTGTAATGAGCATTTTTCCTTCACAATGCTGCATTTCATTAAGAACCAACTAGAAACGGCTCCACCTCATGAGTTAAAGAATACGTTCCAACTACTTCAT
GAAATATTGGTTATTGAAGATCCTATACAAGcAGAGCGAGTCAAATTTGTGTTTGAGACAGAAAATGGATTACTAGCTTTGATGCACCACAGTAATCATG
TGGACAGTAGTCGCTGCTACCAGTGTGTCAAATTTCTTGTCACTCTTGCTCAAAAGTGTCCTGCAGCTAAGGAGTACTTCAAGGAGAATTCCCACCACTG
GAGCTGGGCTGTGCAGTGGCTACAGAAGAAGATGTCAGAACATTACTGGACACCACAGAGTAATGTCTCTAATGAAACATCAACTGGAAAAACCTTTCAG
CGAACCATTTCAGCTCAGGACACGTTAGCGTATGCCACAGCTTTGTTGAATGAAAAAGAGCAATCAGGAAGCAGTAATGGGTCGGAGAGTAGTCCTGCCA
ATGAGAACGGAGACAGGCATCTACAGCAGGGTTCAGAATCTCCCATGATGATTGGTGAGTTGAGAAGTGACCTTGATGATGTTGATCCCTAGgcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSRKGTIDAAAMTTLLCMGFSDPATIRKALRLAKNDINEAVALLTNERPGLDYGGYE
PMDSGGLSESDENCRRFMDRCMPEAFKKLLTSSAVHKWGTEIHEGIYNMLMLLIELVAERIKQDPIPTGLLGVLTMAFNPDNEYHFKNRMKVSQRNWAEV
FGEGNMFAVSPVSTFQKEPHGWVVDLVNKFGELGGFAAIQAKLHSEDIELGAVSALIQPLGVCAEYLNSSVVQPMLDPVILTTIQDVRSVEEKDLKDKRL
VSIPELLSAVKLLCMRFQPDLVTIVDDLRLDILLRMLKSPHFSAKMNSLKEVTKLIEDSTLSKSVKNAIDTDRLLDWLVENSVLSIALEGNIDQAQYCDR
IKGIIELLGSKLSLDELTKIWKIQSGQSSTVIENIHTIIAAAAVKFNSDQLNHLFVLIQKVLDVLWELAHLPTLPSSLIQQALEEHLTILSDAYAVKEAI
KRSYIIKCIEDIKRSSQLNNPQFVWVVPALRQLHEITRSFIKQTYQKQDKSIIQDLKKNFEIVKLVTGSLIACHRLAAAVAGPGGLSGSTLVDGRYTYRE
YLEAHLKFLAFFLQEATLYLGWNRAKEIWECLVTGQDVCELDREMCFEWFTKGQHDLESDVQQQLFKEKILKLESYEITMNGFNLFKTFFENVNLCDHRL
KRQGAQLYVEKLELIGMDFIWKIAMESPDEEIANEAIQLIINYSYINLNPRLKKDSVSLHKKFIADCYTRLEAASSALGGPTLTHAVTRATKMLTATAMP
TVATSVQSPYRSTKLVIIERLLLLAERYVITIEDFYSVPRTILPHGASFHGHLLTLNVTYESTKDTFTVEAHSNETIGSVRWKIAKQLCSPVDNIQIFTN
DSLLTVNKDQKLLHQLGFSDEQILTVKTSGSGTPSGSSADSSTSSSSSSSGVFSSSYAMEQEKSLPGVVMALVCNVFDMLYQLANLEEPRITLRVRKLLL
LIPTDPAIQEALDQLDSLGRKKTLLSESSSQSSKSPSLSSKQQHQPSASSILESLFRSFAPGMSTFRVLYNLEVLSSKLMPTADDDMARSCAKSFCENFL
KAGGLSLVVNVMQRDSIPSEVDYETRQGVYSICLQLARFLLVGQTMPTLLDEDLTKDGIEALSSRPFRNVSRQTSRQMSLCGTPEKSSYRQLSVSDRSSI
RVEEIIPAARVAIQTMEVSDFTSTVACFMRLSWAAAAGRLDLVGSSQPIKESNSLCPAGIRNRLSSSGSNCSSGSEGEPVALHAGICVRQQSVSTKDSLI
AGEALSLLVTCLQLRSQQLASFYNLPCVADFIIDILLGSPSAEIRRVACDQLYTLSQTDTSAHPDVQKPNQFLLGVILTAQLPLWSPTSIMRGVNQRLLS
QCMEYFDLRCQLLDDLTTSEMEQLRISPATMLEDEITWLDNFEPNRTAECETSEADNILLAGHLRLIKTLLSLCGAEKEMLGSSLIKPLLDDFLFRASRI
ILNSHSPAGSAAISQQDFHPKCSTANSRLAAYEVLVMLADSSPSNLQIIIKELLSMHHQPDPALTKEFDYLPPVDSRSSSGFVGLRNGGATCYMNAVFQQ
LYMQPGLPESLLSVDDDTDNPDDSVFYQVQSLFGHLMESKLQYYVPENFWKIFKMWNKELYVREQQDAYEFFTSLIDQMDEYLKKMGRDQIFKNTFQGIY
SDQKICKDCPHRYEREEAFMALNLGVTSCQSLEISLDQFVRGEVLEGSNAYYCEKCKEKRITVKRTCIKSLPSVLVIHLMRFGFDWESGRSIKYDEQIRF
PWMLNMEPYTVSGMARQDSSSEVGENGRSVDQGGGGSPRKKVALTENYELVGVIVHSGQAHAGHYYSFIKDRRGCGKGKWYKFNDTVIEEFDLNDETLEY
ECFGGEYRPKVYDQTNPYTDVRRRYWNAYMLFYQRVSDQNSPVLPKKSRVSVVRQEAEDLSLSAPSSPEISPQSSPRPHRPNNDRLSILTKLVKKGEKKG
LFVEKMPARIYQMVRDENLKFMKNRDVYSSDYFSFVLSLASLNATKLKHPYYPCMAKVSLQLAIQFLFQTYLRTKKKLRVDTEEWIATIEALLSKSFDAC
QWLVEYFISSEGRELIKIFLLECNVREVRVAVATILEKTLDSALFYQDKLKSLHQLLEVLLALLDKDVPENCKNCAQYFFLFNTFVQKQGIRAGDLLLRH
SALRHMISFLLGASRQNNQIRRWSSAQAREFGNLHNTVALLVLHSDVSSQRNVAPGIFKQRPPISIAPSSPLLPLHEEVEALLFMSEGKPYLLEVMFALR
ELTGSLLALIEMVVYCCFCNEHFSFTMLHFIKNQLETAPPHELKNTFQLLHEILVIEDPIQAERVKFVFETENGLLALMHHSNHVDSSRCYQCVKFLVTL
AQKCPAAKEYFKENSHHWSWAVQWLQKKMSEHYWTPQSNVSNETSTGKTFQRTISAQDTLAYATALLNEKEQSGSSNGSESSPANENGDRHLQQGSESPM
MIGELRSDLDDVDP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28020
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Transcript variant 2 - compared to NCBI ref has 8 residues missing at the extreme N-terminus plus 3 internal deletions (aa61-164) (aa519-550) and (aa605-620). V2308A in variant 2 corresponds to V2468A in NCBI ref.; Parental 33042 is also Transcript variant 2